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pAAVS1-Puro-DNR

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SKU
GE100024
Donor vector with AAVS1 homologous arms, C-terminal Myc-DDK tag, Puro resistance, ready for transgene cloning
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function AAVS1 donor vector
E. coli Selection Ampicillin
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • Puromycin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping Ambient
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Documents
Resources

file-alt Citations

Engineering Receptor-Directed Intracellular Palladium Catalysis
Ghosh
ChemRxiv (2026)
Snippet: For later restriction and ligation into a pAAVS1-puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR
NI-3201 Is a Bispecific Antibody Mediating PD-L1–Dependent CD28 Co-stimulation on T Cells for Enhanced Tumor Control
Majocchi, Lloveras, Nouveau et al
Cancer Immunology Research (2025)
Cited by 11
AI Summary: OriGene’s pAAVS1-Puro-DNR vector (Cat. #GE100024) was used to generate stable MC38 cell clones expressing human CEA, enabling key in vitro and in vivo experiments to assess the efficacy of the NI-3201 bispecific antibody in this study.
Snippet: huCEA-MC38 corresponds to an engineered MC38 clonal cell line expressing the CEA protein at its surface. It was generated by cloning the human CEA full-length gene (accession number NM_004363.2) into the pAAVS1-Puro-DNR vector (OriGene, Cat. #GE100024) allowing for the targeted transgene insertion into the human AAVS1 locus. …
Enhancing Peptide and PMO Delivery to Mouse Airway Epithelia by Chemical Conjugation with the Amphiphilic Peptide S10
Auger, Sorroza-Martinez, Brahiti et al
Molecular Therapy - Nucleic Acids (2024)
Cited by 2
AI Summary: OriGene products, specifically the AAVS1 targeting vectors (CAT#: GE100023 and customized GE100024), were used to introduce an EGFP reporter cassette and selection marker into cells, enabling the generation and validation of modified cell lines for the study.
Snippet: resistant gene, an EGFP544 654 reporter cassette, and a homology arms to the AAVS1 locus sequence (OriGene 545 Technologies, CAT#: GE100023 and customized GE100024). Cells were selected for 546 puromycin resistance and single clones were isolated, validated, and used for in
A Novel Role for ACE2-TMEM16 Complexes in SARS-CoV-2 Infection
Vidmar
Thesis (2024)
Snippet: U2OS and Vero cell lines stably expressing ACE2-eGFP were obtained through the Transgene Knockin via CRISPR at AAVS1 and ROSA26 Loci Kit (GE100027 Origene). We used ACE2eGFP (154962 AddGene) for the insert, while the pAAVS1-Puro-DNR (GE100024) was the donor. … Once obtained the plasmid through cloning, we followed the manufacturer’s instruction transfecting 1ug of donor and 1ug of GE100023 pCas-Guide-AAVS1 diluting them in Opti-MEM (Life Technologies), m
Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII
Ramamurthy, Rodriguez, Ainsworth et al
Front Immunol (2022) 13, 954984 DOI: 10.3389/fimmu.2022.954984
Cited by 4
AI Summary: OriGene products, specifically the pCas-Guide-AAVS1 (GE100023) and pAAVS1-puro-DNR (GE100024) plasmids, were used as key tools for CRISPR/Cas9-mediated insertion attempts of the fVIII transgene into the AAVS1 genomic safe harbor in this study.
Snippet: ator with 5% CO2. Plasmid construction and synthesis An AAVS1 transgene knock-in vector kit, consisting of the pCas-Guide-AAVS1 (GE100023) and pAAVS1-puro-DNR (GE100024) plasmids, was purchased from OriGene Technologies, Inc. (Rockville, MD). A “cassette” containing the bioengineered fVIII transgene “lcoET3” under the transcriptional control of the constitutively active human EF1α promot
FigureFigureFigure
GigaAssay - An adaptable high-throughput saturation mutagenesis assay platform
Benjamin, Giacoletto, FitzHugh et al
Genomics (2022) 114 (4), 110439 DOI: 10.1016/j.ygeno.2022.110439
Cited by 13
AI Summary: In this study, the pAAVS1-Puro-DNR vector from OriGene was used as a cloning backbone for constructing reporter cell lines essential to the development and validation of the GigaAssay high-throughput mutagenesis platform.
Snippet: P and LentiCRISPR-v2 Blast (Addgene) as templates. LTR, GFP, and bsr amplicons were fused by inverse PCR using Q5 High-Fidelity DNA Polymerase. The fused amplicons were cloned into pAAVS1-Puro-DNR (Origene) previously digested with SpeI and EcoRI. For lentiviral constructs to test doubles insertions, a NheI-KpnI fragment containing ZsGreen1-DR-PPT-PGK promoter-HygB and a similar NheI-MluI fragme
Showing 1-6 of 13 papers.
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