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pAAVS1-EF1a-puro-DNR CRISPR Vector

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SKU
GE100046
AAVS1 donor vector with AAVS1 homologous arms cloned (Puro selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function AAVS1 donor vector
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • The expression of the target gene will be under EF1a promoter, a constitutive promoter as an alternative of CMV promoter. the latter could be silenced in certain cellular environment
  • Puromycin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus
Lee, Cho, Lee et al
Diabetol Metab Syndr (2024) 16 (1), 149 DOI: 10.1186/s13098-024-01390-0
Cited by 2
AI Summary: OriGene’s Parkin and MsrB2 ORF clones were used in this study to generate RFP- and GFP-tagged fusion proteins, enabling the investigation of their roles in mitophagy and cardiac protection in diabetic cardiomyopathy.
Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively. Cherry-LC3 was purchased by;cytes were cultured in a serum-free medium for ≥ 4 h and treated with 100 ng Endothelin-1 (ET-1) or 25 mM glucose for 48 h. Transient transfection We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP...
FigureFigureFigure
Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions
Misra, Carlson, Spandau et al
Nucleic Acids Res (2024) 52 (4), 1830-1846 DOI: 10.1093/nar/gkae006
Cited by 63
AI Summary: OriGene products pCas-Guide-AAVS1 (Cat #GE100023) and pAAVS1EF1a-Puro-DNR (Cat #GE100046) plasmids were used for plasmid constructions in the study investigating mechanisms of GCN2 activation under stress conditions.
Snippet: Plasmid constructions The pCas-Guide-AAVS1(Cat #GE100023) and pAAVS1EF1a-Puro-DNR (Cat #GE100046) plasmids were obtained from Origene. A segment of
Transplantation of hMSCs Genome Edited with LEF1 Improves Cardio-Protective Effects in Myocardial Infarction
Cho, Lee, Shen et al
Mol Ther Nucleic Acids (2020) 19, 1186-1197 DOI: 10.1016/j.omtn.2020.01.007
Cited by 45
AI Summary: In this study, OriGene products including Human LEF1 cDNA (RC201375) and Anti-LEF1 antibody (TA324324) were used to overexpress and detect LEF1 in human mesenchymal stem cells, enabling the researchers to assess the therapeutic effects of LEF1-engineered cells in a rat model of myocardial infarction.
FigureFigureFigure
Showing 1-3 of 3 papers.
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