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pAAVS1-mGFP-BSD-DNR

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SKU
GE100065
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Blasticidin resistance, ready for transgene cloning
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function AAVS1 donor vector
Features

Donor vector for targeted transgene insertion into human AAVS1 site

  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • Blasticidin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (GE100023)
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

The Calcium Channel Subunit Gamma-4 as a Novel Regulator of MafA in Pancreatic Beta-Cell Controls Glucose Homeostasis
Wu, Karagiannopoulos, Eliasson et al
Biomedicines (2022) 10 (4) DOI: 10.3390/biomedicines10040770
Cited by 5
AI Summary: OriGene’s lentiviral plasmids encoding Cacng4 with mGFP tag and control plasmids were used for transfection experiments to study the regulatory role of the calcium channel subunit gamma-4 in pancreatic beta-cell function and glucose homeostasis.
Snippet: previous study [22]. 2.7. Lentiviral Transfection Cacng4 or control (lentiviral particles without targeting any specific region) plasmids cloned in Lentiviral based shuttle vectors with mGFP tagged (ORIGENE, Rockville, MA, USA) were transformed into E. coli on LB-agar plates supplemented with 34 μg/mL chloramphenicol. Amplified plasmids were purified with QIAGEN Plasmid Midi Kit (QIAGEN, Hilden,;...
FigureFigureFigure
ZEB1 transcription factor promotes immune escape in melanoma
Plaschka, Benboubker, Grimont et al
J Immunother Cancer (2022) 10 (3) DOI: 10.1136/jitc-2021-003484
Cited by 109
AI Summary: OriGene’s Cxcl10 (NM_021274) Mouse Tagged ORF Clone – MR200291L2 plasmid, tagged with mGFP, was used in this study to manipulate chemokine expression in melanoma cells and investigate the role of ZEB1 in immune evasion.
Snippet: ression constructs (10 µg) in combination with GAG-POL (5 µg) and ENV expression vectors (10 µg). Cxcl10 (NM_021274) Mouse Tagged ORF Clone – MR200291L2 plasmid used were tagged with mGFP (OriGene Technologies, Inc, Rockville, US). Viral supernatants were collected 48 hours post-transfection, filtered (0.45 µm membrane) and placed in contact with 2×106 melanoma cells for 8 hours i
The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification
Luan, Ye, Singh et al
Commun Biol (2019) 2, 106 DOI: 10.1038/s42003-019-0351-4
AI Summary: OriGene’s lentiviral plasmids encoding Cacng4 with mGFP tag were used to manipulate CaVγ4 expression in cells, enabling the authors to investigate its role in pancreatic beta-cell specification and function.
Snippet: ontrol (Invitrogen, USA). Lentiviral transfection Cacng4 or control (lentiviral particles without targeting any specific region) plasmids cloned in Lentiviral based shuttle vectors with mGFP tagged (ORIGENE, USA) were transformed into E. coli on LB-agar plates supplemented with 34 μg ml−1 chloramphenicol. Amplified plasmids were purified with QIAGEN Plasmid Midi Kit (QIAGEN, USA). Lentivir
FigureFigureFigure
Showing 1-3 of 3 papers.
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