AAVS1 Transgene Knockin kit (BSD)
SKU
GE100036
Vector kit for targeted transgene insertion into AAVS1 locus (blasticidin selection), including AAVS1 gRNA vector (cat# GE100023), AAVS1-BSD-donor (cat# GE100035) and gRNA scramble control (cat# GE100003)
$1,290.00
2 Weeks*
Specifications
| Product Data | |
| Function | CRISPR/AAVS1 kit |
|---|---|
| Features | Complete vector kit for targeted transgene insertion into human AAVS1 site via CRISPR Components: Transgene needs to be cloned into pAAVS1-BSD-DNR |
| Storage | Please store at -20°C for long term storage. |
| Stability | The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C. |
| Shipping | Ambient |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Fc-competent multispecific PDL-1/TIGIT/LAG-3 antibodies potentiate superior anti-tumor T cell response
Yang, Huang, Huang et al
Sci Rep (2023) 13 (1), 9865 DOI: 10.1038/s41598-023-36942-3
Yang, Huang, Huang et al
Sci Rep (2023) 13 (1), 9865 DOI: 10.1038/s41598-023-36942-3
Cited by 21
AI Summary: OriGene's AAVS1 Transgene Knockin kit was used with CRISPR-Cas9 technology to generate stable cell lines expressing target transgenes for in vitro experiments in this study.
Snippet: bodies were then filtered with a 0.22 µM membrane and used for experiments. Generation of stable cell lines Transgenes were targeted to the AAVS1 safe harbor using the AAVS1 Transgene Knockin kit (Origene) with CRISPR-Cas9 knock-in technology for stable expression. Briefly, the coding region was amplified from template with primers (Table S7) by PCR using Q5 Hot Start High-Fidelity DNA Polymera
ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
Cook, Sengelmann, Winkler et al
Int J Mol Sci (2022) 23 (4) DOI: 10.3390/ijms23041976
Cook, Sengelmann, Winkler et al
Int J Mol Sci (2022) 23 (4) DOI: 10.3390/ijms23041976
Cited by 22
AI Summary: OriGene products, specifically the ADAM8 Human Gene Knockout Kit (CRISPR) (CAT#: KN213386) and the ADAM8 AAVS1 Transgene Knockin Kit (CAT#: GE100036), were used to generate and manipulate ADAM8 knockout and transgenic cell lines, enabling the study of ADAM8’s role in tumor microenvironment signaling in this paper.
Snippet: … Generation of Stable Panc89 and MB-231 ADAM8 Knockout Cells To generate a genomic ADAM8 knockout in Panc89 and MB-231 cells, the ADAM8 Human Gene Knockout Kit (CRISPR) from OriGene (CAT#: KN213386, Rockville, ML, USA) was used. … Single-cell clones were obtained after the;the ADAM8 AAVS1 Transgene Kockin kit (BSD) from OriGene (CAT#: GE100036, Rockville, ML, USA) was used
Metalloprotease inhibitor profiles of human ADAM8 in vitro and in cell-based assays
Schlomann, Dorzweiler, Nuti et al
Biol Chem (2019) 400 (6), 801-810 DOI: 10.1515/hsz-2018-0396
Schlomann, Dorzweiler, Nuti et al
Biol Chem (2019) 400 (6), 801-810 DOI: 10.1515/hsz-2018-0396
Cited by 33
AI Summary: In this study, OriGene’s recombinant human ADAM8 protein (Cat# TP317435) and human ADAM8 cDNA clone (Cat# RC210865) were used to assess ADAM8 enzymatic activity and its inhibition profile in both in vitro and cell-based assays.
Showing 1-3 of 3 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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