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AAVS1 Transgene knockin vector kit

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SKU
GE100027
Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003
  $1,290.00
In Stock*

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Specifications
Specifications
Product Data
Function CRISPR kit
Features
  • Complete vector kit for targeted transgene insertion into human AAVS1 site via CRISPR
  • Components:
  • Transgene needs to be cloned into pAAVS1-puro-DNR
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

A Novel Role for ACE2-TMEM16 Complexes in SARS-CoV-2 Infection
Vidmar
Thesis (2024)
Snippet: U2OS and Vero cell lines stably expressing ACE2-eGFP were obtained through the Transgene Knockin via CRISPR at AAVS1 and ROSA26 Loci Kit (GE100027 Origene). We used ACE2eGFP (154962 AddGene) for the insert, while the pAAVS1-Puro-DNR (GE100024) was the donor. … Once obtained the plasmid through cloning, we followed the manufacturer’s instruction transfecting 1ug of donor and 1ug of GE100023 pCas-Guide-AAVS1 diluting them in Opti-MEM (Life Technologies), m
Photothermal Transfection for Effective Nonviral Genome Editing
Oh, Park, Kim et al
ACS Appl Bio Mater (2021) 4 (7), 5678-5685 DOI: 10.1021/acsabm.1c00465
Cited by 6
AI Summary: In this study, OriGene’s pCMV6-Entry plasmid DNA was used as the nucleic acid cargo for transfection experiments, enabling the evaluation of the photothermal transfection platform’s efficiency in delivering plasmid DNA into human primary cells.
Photothermal Transfection for Effective Nonviral Genome Editing
Oh, Park, Kim et al
ACS Appl. Bio Mater. (2021) 4 (7), 5678-5685 DOI: 10.1021/acsabm.1c00465
Cited by 6
AI Summary: OriGene’s AAVS1 Transgene knockin vector kit CRISPR Vector (GE100027), including plasmids for Cas9 expression and AAVS1-targeting gRNA, was used to facilitate genome editing experiments in this study.
Snippet: The AAVS1 Transgene knockin vector kit CRISPR Vector (GE100027, Origene, USA) consisting of plasmids with Cas9 expression and gRNA targeting the AAVS1 locus (pCas-Guide-AAVS1) and plasmids with a puromycin resistance marker for transgene integration
Showing 1-3 of 3 papers.
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