OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

pCas-Guide-Puro-CRISPRi gene interference vector

Be the first to review this product
SKU
GE100083
pCas-Guide-Puro-CRISPRi Vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker, dCas9-KRAB-MeCP2 and gRNA cloning site.
  $670.00
In Stock*

Frequently Bought Together (4)

TA811179 CAS9 mouse monoclonal antibody,clone OTI5E5 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
500 pmol
$150.00
TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
500 pmol
$160.00
Specifications
Specifications
Product Data
Features
  • All-in-one CRISPRi vector system
  • gRNA driven by U6 promoter (after target sequence cloned)
  • dCas9-KRAB-MeCP2 fusion protein expression by CMV promoter
  • CF3 primer can be used to sequence target sequence after cloning
  • Contains puromycin resistance gene under SV40 promoter
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping Ambient
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Rewiring of SINE-MIR enhancer topology and Esrrb modulation in expanded and naive pluripotency
Cipta, Zeng, Wong et al
Genome Biol (2025) 26 (1), 107 DOI: 10.1186/s13059-025-03577-8
Cited by 6
AI Summary: OriGene’s pCas9-Guide-Puro-CRISPRi-Vector (GE100083) was used as the backbone for CRISPR interference experiments to investigate the functional role of MIR enhancers in pluripotency regulation.
Snippet: for their off-target scores. sgRNA sequences were added with an overhang sequence and ordered as DNA oligos from IDT and cloned into pCas9-Guide-Puro-CRISPRi-Vector. CRISPRi vector was obtained from ORIGENE (GE100083) and used according to the manufacturer’s protocol, with minor modifications. One thousand-nanogram vector plasmid was digested with 16U BamHI-HF (NEB) and 8U BsmBI-v2 (NEB) enzyme
FigureFigureFigure
TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development
Feng, Han, Yuan et al
Elife (2022) 11 DOI: 10.7554/eLife.80405
AI Summary: OriGene products, specifically the pCas-Guide-Puro-CRISPRi plasmid (GE100083) for Fgf18 targeting and the pCas-Guide-Puro-CRISPRi-Scramble plasmid (GE100084) as a control, were used to manipulate Fgf18 expression and investigate its role in pharyngeal muscle development in this study.
Snippet: … This gRNA sequence was then cloned to pCas-Guide-Puro-CRISPRi plasmid (GE100083, Origene Technologies) by Origene Technologies to generate a Fgf18 CRISPRi Plasmid. pCas-Guide-Puro-CRISPRi-Scramble plasmid (GE100084, Origene Technologies) was used as control. … This gRNA sequence was then cloned to pCas-Guide-Puro-CRISPRi plasmid (GE100083, Origene Technologies) by Origene Technologies to generate a Fgf18 CRISPRi Plasmid. pCas-Guide-Puro-CRISPRi-Scramble plasmid (GE100084, Origene Technologies) was used as control
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products