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pAAVS1-EF1a-BSD-DNR

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SKU
GE100048
AAVS1 donor vector with AAVS1 homologous arms cloned (Blasticidin selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
  $880.00
In Stock*

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Specifications
Specifications
Product Data
Function AAVS1 donor vector
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • The expression of the target gene will be under EF1a promoter, a constitutive promoter as an alternative of CMV promoter. the latter could be silenced in certain cellular environment
  • Blasticidin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
Shipping Ambient
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Documents
Resources

file-alt Citations

CRISPR RNA architecture steers Cas9 catalysis and fidelity via a guide repeat clasp motif
Chilamkurthy, Sudhakar, Pater et al
Nucleic Acids Res (2026) 54 (15) DOI: 10.1093/nar/gkag710
AI Summary: In this study, the pAAVS1-EF1a-BSD-DNR vector (OriGene, GE100048) from OriGene was used as a backbone for EGFP gene insertion, facilitating the creation of reporter constructs to investigate CRISPR-Cas9 guide RNA architecture and editing fidelity.
Snippet: lternative codon-optimized version of EGFP. The EGFP gene was synthesized as a gBlock (Integrated DNA Technologies, IDT) with 15 bp extensions complementary to the ends of pAAVS1-EF1a-BSD-DNR vector (ORIGENE, GE100048) after digestion with KpnI (Thermo Scientific). Insertion
A guide RNA repeat checkpoint steers CRISPR-Cas9 catalysis
Chilamkurthy, Sudhakar, Pater et al
bioRxiv (2025)
AI Summary: OriGene's pAAVS1-EF1a-BSD-DNR vector (GE100048) was used as the backbone for generating HEK 293T cells stably expressing an alternative codon-optimized EGFP, facilitating CRISPR-based gene knock-in experiments in this study.
Snippet: In addition, we used CRISPR based AAVS1 safe harbor knock-in for generating HEK 293T cells stably expressing an alternative codon-optimized version of EGFP. The EGFP gene was synthesized as a gBlock (Integrated DNA Technologies, IDT) with 15 bp extensions complementary to the ends of pAAVS1-EF1a-BSD-DNR vector (ORIGENE, GE100048) after digestion with KpnI
Showing 1-2 of 2 papers.
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