Autoimmunity

Western blot with Porcine plasma and purified Porcine CRP (poCRP). Porcine plasma was diluted 1/25 and spiked with 0.08 or 0.3ug purified Porcine CRP, respectively. Polyacrylamide gel electrophoresis was performed under reducing and denaturing conditions on a 10-20% gel (Novex), blotted onto nitrocellulose and developed with monoclonal antibody PC1 (left) or competitor's monoclonal antibody (right). Lane A: Porcine plasma spiked with 0.08ug poCRP; lane B: 0.08ug poCRP; lane C: Porcine plasma spiked with 0.3ug poCRP; lane D: Porcine plasma; lane E: 0.3ug poCRP. Monoclonal antibody PC1 barely reacts with with the 50-55kDa bands visible on the right, presumably immunoglobulin heavy chains. Gel was slightly overloaded to allow visibility of CRP band in plasma.

Circular map for MR215629L4

Circular map for RC218518L4

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human IL1B plasmid (RC202079, hIL1B), mouse IL1B plasmid (MR226719, mIL1B), rat IL1B plasmid (RR211023, rIL1B) using anti-IL1B antibody TA506443 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Circular map for RC213277

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1F9 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ESR1 (Cat# RC213277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1(Cat# TA503759). Positive lysates LY400046 (100ug) and LC400046 (20ug) can be purchased separately from OriGene.

Circular map for RC202078

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Coomassie blue staining of purified HLA-G protein (Cat# TP305216). The protein was produced from HEK293T cells transfected with HLA-G cDNA clone (Cat# RC205216) using MegaTran 2.0 (Cat# TT210002).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL1RN (Cat# RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN(Cat# TA803422). Positive lysates LY403571 (100ug) and LC403571 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL6R (Cat# RC221874, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL6R antibody (Cat# TA506859). Positive lysates LY400192 (100ug) and LC400192 (20ug) can be purchased separately from OriGene.

Coomassie blue staining of purified ESR1 protein (Cat# TP313277). The protein was produced from HEK293T cells transfected with ESR1 cDNA clone (Cat# RC213277) using MegaTran 2.0 (Cat# TT210002).

CSRP3 is the substrate of O-glycosidase, according to Western blot (WB) and Amaranthus caudatus lectin blot (LB) analyses of recombinant human CSRP3. Recombinant proteins expressed in E. coli (unglycosylated negative control) and HEK293 cells (potentially glycosylated reference) (OriGene TP325217) were analyzed after treatment with sialidase and O-glycosidase. Figure cited from PLoS ONE, PMID: 27281159

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD74 plasmid (RC212964, hCD74), mouse CD74 plasmid (MR225627, mCD74) using anti-CD74 antibody TA507339 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Circular map for RC221874

Circular map for RC202079

Circular map for RC205216

Circular map for RC202716

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 2.5 min, UM800123) (1:1600)

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ESR1 (RC213277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1 (1:500).

Circular map for RC200227

Circular map for RC215123

Circular map for RC204638

Circular map for RC205882

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

Coomassie blue staining of purified HLA-A protein (Cat# TP300661). The protein was produced from HEK293T cells transfected with HLA-A cDNA clone (Cat# RC200661) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC201397

Circular map for RC218519

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ESR1 (Cat# RC213277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1(Cat# TA503759). Positive lysates LY400046 (100ug) and LC400046 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL1RN (Cat# RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN(Cat# TA803422). Positive lysates LY403571 (100ug) and LC403571 (20ug) can be purchased separately from OriGene.

Coomassie blue staining of purified TNF protein (Cat# TP306983). The protein was produced from HEK293T cells transfected with TNF cDNA clone (Cat# RC206983) using MegaTran 2.0 (Cat# TT210002).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1F9 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Circular map for RC218764

Circular map for RC201137

Circular map for RC200661

Coomassie blue staining of purified IL1B protein (Cat# TP302079). The protein was produced from HEK293T cells transfected with IL1B cDNA clone (Cat# RC202079) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC205939

Circular map for RC206983

Circular map for RC217958

Coomassie blue staining of purified IL1RAP protein (Cat# TP308786). The protein was produced from HEK293T cells transfected with IL1RAP cDNA clone (Cat# RC208786) using MegaTran 2.0 (Cat# TT210002).

RC211970L3 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC211970L3V particle to overexpress human IL1RAP-Myc-DDK fusion protein.

Coomassie blue staining of purified HLA-E protein (Cat# TP305939). The protein was produced from HEK293T cells transfected with HLA-E cDNA clone (Cat# RC205939) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC202084

Circular map for RC212964