IL1 beta (IL1B) (NM_000576) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IL1 beta |
| Synonyms | IL-1; IL1-BETA; IL1beta; IL1F2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000576 |
| ORF Size | 807 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000576.3 |
| RefSeq Size | 1498 bp |
| RefSeq ORF | 810 bp |
| Locus ID | 3553 |
| UniProt ID | P01584 |
| Cytogenetics | 2q14.1 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus |
| MW | 30.7 kDa |
| Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. provided by RefSeq, Jul 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000576" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202079L1 | Lenti ORF clone of Human interleukin 1, beta (IL1B), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202079L2 | Lenti ORF clone of Human interleukin 1, beta (IL1B), mGFP tagged | 10 ug |
$750.00
|
|
| RC202079L3 | Lenti ORF clone of Human interleukin 1, beta (IL1B), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202079L4 | Lenti ORF clone of Human interleukin 1, beta (IL1B), mGFP tagged | 10 ug |
$750.00
|
|
| RG202079 | IL1B (tGFP-tagged) - Human interleukin 1, beta (IL1B) | 10 ug |
$650.00
|
|
| SC122566 | IL1B (untagged)-Human interleukin 1, beta (IL1B) | 10 ug |
$450.00
|
|
Citations
Blocking SphK/S1P/S1PR1 axis signaling pathway alleviates remifentanil-induced hyperalgesia in rats
Li, Wang, Gao et al
Neurosci Lett (2023) 801, 137131 DOI: 10.1016/j.neulet.2023.137131
Li, Wang, Gao et al
Neurosci Lett (2023) 801, 137131 DOI: 10.1016/j.neulet.2023.137131
Cited by 9
AI Summary: OriGene products contributed to this study by providing the rabbit anti-S1PR1 antibody (cat. no. TA306649) and rabbit anti-IL-1β antibody (cat. no. RC202079), which were used to detect the expression of S1PR1 and IL-1β in rat spinal cord tissues to investigate the mechanisms underlying remifentanil-induced hyperalgesia.
Amino acid starvation sensing dampens IL-1β production by activating riboclustering and autophagy
Battu, Afroz, Giddaluru et al
PLoS Biol. (2018) 16 (4), e2005317 DOI: 10.1371/journal.pbio.2005317
Battu, Afroz, Giddaluru et al
PLoS Biol. (2018) 16 (4), e2005317 DOI: 10.1371/journal.pbio.2005317
AI Summary: OriGene’s pCMV6 IL-1β plasmid was used in this study to investigate the regulation of IL-1β production under amino acid starvation conditions.
Snippet: ml. Rapamycin (Sigma-Aldrich) was used at a concentration of 200 nM. Lipofectamine 3000 was from Life Technologies (L3000015) and Sepharose G beads from Sigma-Aldrich. pCMV6 IL-1β was purchased from Origene. Generation of murine BMDMs Bone marrow from femurs and tibiae of C57BL/6J mice were flushed with ice-cold PBS/RPMI and cultured at a density of 5 × 106 to 7 × 106 cells in 10-cm Petri dish
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
