IL1 beta (IL1B) (NM_000576) Human Untagged Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | IL1 beta |
| Synonyms | IL-1; IL1-BETA; IL1beta; IL1F2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000576 |
| Insert Size | 1522 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000576.2, NP_000567.1 |
| RefSeq Size | 1498 bp |
| RefSeq ORF | 810 bp |
| Locus ID | 3553 |
| UniProt ID | P01584 |
| Cytogenetics | 2q14.1 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus |
| Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. provided by RefSeq, Jul 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000576" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202079 | IL1B (Myc-DDK-tagged)-Human interleukin 1, beta (IL1B) | 10 ug |
$450.00
|
|
| RC202079L1 | Lenti ORF clone of Human interleukin 1, beta (IL1B), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202079L2 | Lenti ORF clone of Human interleukin 1, beta (IL1B), mGFP tagged | 10 ug |
$750.00
|
|
| RC202079L3 | Lenti ORF clone of Human interleukin 1, beta (IL1B), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202079L4 | Lenti ORF clone of Human interleukin 1, beta (IL1B), mGFP tagged | 10 ug |
$750.00
|
|
| RG202079 | IL1B (tGFP-tagged) - Human interleukin 1, beta (IL1B) | 10 ug |
$650.00
|
|
Citations
The Homeoprotein DLX4 Stimulates NF-κB Activation and CD44-Mediated Tumor-Mesothelial Cell Interactions in Ovarian Cancer
Haria, Trinh, Ko et al
Am. J. Pathol. (2015)
Haria, Trinh, Ko et al
Am. J. Pathol. (2015)
Cited by 34
Caspase-1 and IL-1β processing in a teleost fish
Reis, do Vale, Pereira et al
PLoS ONE (2012) 7 (11), e50450 DOI: 10.1371/journal.pone.0050450
Reis, do Vale, Pereira et al
PLoS ONE (2012) 7 (11), e50450 DOI: 10.1371/journal.pone.0050450
Cited by 89
AI Summary: OriGene contributed to this study by providing the HSpCMV-XL5proIL1β (IL-1β Human cDNA clone, NM_000576.2, in pCMV-XL5 plasmid, #SC122566), which was used for site-directed mutagenesis experiments to investigate IL-1β processing.
Snippet: was mutated to alanine by site-directed mutagenesis using HSpCMV-XL5proIL1β (IL-1β Human cDNA clone (NM_000576.2) in pCMV-XL5 plasmid from Origene (#SC122566)) as ... 4A). Fragment 3 was a short fragment from the N-terminus (GSSHHHHHHM 1 ADK;band 3 in Fig
Intracellular interleukin-1alpha mediates interleukin-8 production induced by Chlamydia trachomatis infection via a mechanism independent of type I interleukin-1 receptor
Cheng, Shivshankar, Zhong et al
Infect Immun (2008) 76 (3), 942-51 DOI: 10.1128/IAI.01313-07
Cheng, Shivshankar, Zhong et al
Infect Immun (2008) 76 (3), 942-51 DOI: 10.1128/IAI.01313-07
AI Summary: OriGene products contributed to this study by providing IL-1alpha-specific small interfering RNA (siRNA) used to knock down IL-1alpha expression, enabling the authors to demonstrate the essential role of intracellular IL-1alpha in Chlamydia trachomatis-induced IL-8 production.
Showing 1-3 of 3 papers.
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