IL37 (NM_014439) Human Tagged ORF Clone
SKU
RC204638
IL37 (Myc-DDK-tagged)-Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IL37 |
| Synonyms | FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H; IL-1H4; IL-1RP1; IL-23; IL-37; IL1F7; IL1H4; IL1RP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014439 |
| ORF Size | 654 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014439.3, NP_055254.2 |
| RefSeq Size | 787 bp |
| RefSeq ORF | 657 bp |
| Locus ID | 27178 |
| UniProt ID | Q9NZH6 |
| Cytogenetics | 2q14.1 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 24.1 kDa |
| Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014439" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204638L1 | Lenti ORF clone of Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204638L2 | Lenti ORF clone of Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC204638L3 | Lenti ORF clone of Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204638L4 | Lenti ORF clone of Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG204638 | IL37 (tGFP-tagged) - Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1 | 10 ug |
$650.00
|
|
| SC122809 | IL37 (untagged)-Human interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
NLRP3 inflammasome triggers interleukin-37 release from human monocytes
Gritsenko, Díaz-Pino, López-Castejón
Eur J Immunol (2022) 52 (7), 1141-1157 DOI: 10.1002/eji.202149724
Gritsenko, Díaz-Pino, López-Castejón
Eur J Immunol (2022) 52 (7), 1141-1157 DOI: 10.1002/eji.202149724
Cited by 11
AI Summary: OriGene’s IL-37 Human Myc-tagged ORF clone (RC204638) was used to clone IL-37 isoform B, enabling the generation of IL-37b-cherry THP-1 cell lines that were central to investigating IL-37 release mechanisms in this study.
Snippet: … Generation of IL‐37b‐cherry THP‐1 cell lines IL‐37 isoform B was cloned from IL‐37 Human Myc‐tagged ORF clone (Origene, RC204638) which corresponds to the NM_014439 reference sequence and is referred to as variant 1 [43]. AttB overhangs were added using the following primers obtained from Sigma: Forward: 5‘;IL-37 isoform B was cloned from IL-37 Human Myc-tagged ORF clone (Origene, RC204638) which corresponds to the NM_014439 reference sequence and is referred to as variant 1 [43]. …
Interleukin-37 suppresses tumor growth through inhibition of angiogenesis in non-small cell lung cancer
Ge, Wang, Yang et al
J. Exp. Clin. Cancer Res. (2016) 35 (1), 13 DOI: 10.1186/s13046-016-0293-3
Ge, Wang, Yang et al
J. Exp. Clin. Cancer Res. (2016) 35 (1), 13 DOI: 10.1186/s13046-016-0293-3
Cited by 60
AI Summary: OriGene's Human IL-37 (NM_014439) cDNA open reading frame was used to generate IL-37-overexpressing cell lines, enabling the study of IL-37's effects on tumor growth and angiogenesis in non-small cell lung cancer.
Snippet: Human IL-37 (NM_014439) cDNA open reading frame (Origene Technologies, Beijing, China) were cloned into pcDNA3.1 vector. The IL-37 plasmids or pcDNA3.1 vector were transfected into cells using Lipofectamine 2000. ... Cite SaveSaving
IL-37 Ameliorates the Inflammatory Process in Psoriasis by Suppressing Proinflammatory Cytokine Production
Teng, Hu, Wei et al
J. Immunol. (2014)
Teng, Hu, Wei et al
J. Immunol. (2014)
Cited by 170
AI Summary: In this study, OriGene products pCMV6-entry vector (pNull) and human IL-37 cDNA cloned into pCMV6-entry vector (pIL-37) were used to transiently transfect HaCaT cells, enabling the researchers to assess the effects of IL-37 overexpression on inflammatory cytokine production in psoriasis models.
Snippet: The mock pCMV6- entry (pNull) vector tagged C-terminal Myc-DDK (Origene Technologies, Rockville, MD) and human IL-37 (NM 014439) cDNA open reading frame were cloned into pCMV6-entry vector (pIL-37;Origene Technologies) and were sequenced to exclude ... Cite SaveSaving
Showing 1-3 of 3 papers.
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