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TNF alpha (TNF) (NM_000594) Human Recombinant Protein

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SKU
TP306983
Recombinant protein of human tumor necrosis factor (TNF superfamily, member 2) (TNF), 20 µg
Size:
20 ug 1 mg 100 ug
  $737.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC206983 protein sequence
Red=Cloning site Green=Tags(s)

MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLI
SPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLF
KGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSA
EINRPDYLDFAESGQVYFGIIAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 25.5 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Cell treatment (PMID: 25406462)
Cell treatment (PMID: 28057743)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_000585
Locus ID 7124
UniProt ID P01375
Cytogenetics 6p21.33
RefSeq Size 1686
RefSeq ORF 699
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. provided by RefSeq, Aug 2020
Protein Categories ADC Targets, Allergies and autoimmune diseases, Chemokines, Cytokines, Growth Factors, Intracellular Proteins, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus
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Other "TNF alpha" proteins (11)
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TP700015 Recombinant protein of human soluble form of TNF-alpha with His and DDK tags, expressed in human cells 20 ug
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TP720007 Recombinant protein of human tumor necrosis factor (TNF superfamily, member 2) (TNF), the soluble form (Val77 -Leu233) 10 ug
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TP721024 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
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TP721025 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
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TP723708 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
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TP723983 Recombinant human TNFa protein with C-terminal 6×His tag 100 ug
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TP750007 Recombinant protein of human Tumor Necrosis Factor-a (TNFa) produced in E. coli. 100 ug
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TP750117 Purified recombinant protein of Human tumor necrosis factor (TNF) mutant, tag free, expressed in E.Coli, 50 ug 50 ug
$270.00

file-alt Citations

Effect of tumor necrosis factor-α on the expression of the ammonia transporter Rhcg in the brain in mice with acute liver failure
Wang, Lu, Lu et al
J Neuroinflammation (2018) 15 (1), 234 DOI: 10.1186/s12974-018-1264-8
AI Summary: In this study, OriGene's recombinant TNF-α protein was used to induce acute liver failure in mice, enabling the researchers to investigate the impact of TNF-α on Rhcg expression and ammonia transport in the brain.
Snippet: weight;Sigma) to induce ALF. A second ALF-induction group received intraperitoneal injections of D-GalN at 600 mg/kg body weight and TNF-α (10 μg/kg body weight, Origene, Rockville, MD, USA). Five control groups were Cached;
FigureFigureFigure
IL21R expressing CD14+CD16+ monocytes expand in multiple myeloma patients leading to increased osteoclasts
Bolzoni, Ronchetti, Storti et al
Haematologica (2017)
AI Summary: OriGene’s recombinant human TNF-α (rhTNF-α) was used to treat THP-1 cells, enabling the researchers to study how inflammatory signals affect gene expression related to osteoclast formation in multiple myeloma.
Snippet: Page 8. 7 THP-1 cells were treated with rhIL-6 (20 ng/ml) (Thermo Scientific;Rockford, IL) and/or rh Tumor necrosis factor (TNF)-α (10 ng/ml) (OriGene;Rockville, MD) for 48 h and then collected for mRNA analysis. Lentiviral infections.
Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential
Adini, Adini, Bazinet et al
FASEB J. (2014)
Cited by 30
AI Summary: OriGene contributed recombinant FMOD proteins to this study, which were used to investigate how FMOD regulates MCP-1 secretion and angiogenesis in relation to melanocyte pigmentation.
Snippet: were from Sigma-Aldrich (St. Louis, MO, USA). Recombinant TNF-a and FMOD proteins were purchased from OriGene (Rockville, MD, USA), R&D Systems, and Proteintech Group, Incorporated. Recombinant VEGF-A and MCP ... Cite SaveSaving
Showing 1-3 of 3 papers.
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