OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TNF alpha (TNF) (NM_000594) Human Over-expression Lysate

Be the first to review this product
SKU
LY424626
Transient overexpression lysate of tumor necrosis factor (TNF superfamily, member 2) (TNF) (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA506513 TNF mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "TNF alpha or NM_000594"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206983
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000594, NP_000585
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Predicted MW 25.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000585
Locus ID 7124
Cytogenetics 6p21.33
Protein Families WB
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "TNF alpha" proteins (11)
SKU Description Size Price
PH306983 TNF MS Standard C13 and N15-labeled recombinant protein (NP_000585) 10 ug
$3,360.00
LC424626 Lyophilized TNF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP306983 Recombinant protein of human tumor necrosis factor (TNF superfamily, member 2) (TNF), 20 µg 20 ug
$737.00
TP700015 Recombinant protein of human soluble form of TNF-alpha with His and DDK tags, expressed in human cells 20 ug
$900.00
TP720007 Recombinant protein of human tumor necrosis factor (TNF superfamily, member 2) (TNF), the soluble form (Val77 -Leu233) 10 ug
$205.00
TP721024 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
$180.00
TP721025 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
$340.00
TP723708 Purified recombinant protein of Human tumor necrosis factor (TNF) 10 ug
$345.00
TP723983 Recombinant human TNFa protein with C-terminal 6×His tag 100 ug
$855.00
TP750007 Recombinant protein of human Tumor Necrosis Factor-a (TNFa) produced in E. coli. 100 ug
$540.00
TP750117 Purified recombinant protein of Human tumor necrosis factor (TNF) mutant, tag free, expressed in E.Coli, 50 ug 50 ug
$270.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products