Estrogen Receptor beta (ESR2) (NM_001437) Human Tagged ORF Clone
$740.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Estrogen Receptor beta |
| Synonyms | ER-BETA; Erb; ESR-BETA; ESRB; ESTRB; NR3A2; ODG8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001437 |
| ORF Size | 1590 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001437.2 |
| RefSeq Size | 2011 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 2100 |
| UniProt ID | Q92731 |
| Cytogenetics | 14q23.2-q23.3 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| MW | 59 kDa |
| Summary | This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001437" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218519L1 | Lenti ORF clone of Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a, Myc-DDK-tagged | 10 ug |
$1,040.00
|
|
| RC218519L2 | Lenti ORF clone of Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a, mGFP tagged | 10 ug |
$1,040.00
|
|
| RC218519L3 | Lenti ORF clone of Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a, Myc-DDK-tagged | 10 ug |
$1,040.00
|
|
| RC218519L4 | Lenti ORF clone of Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a, mGFP tagged | 10 ug |
$1,040.00
|
|
| RG218519 | ESR2 (tGFP-tagged) - Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a | 10 ug |
$940.00
|
|
| SC119216 | ESR2 (untagged)-Human estrogen receptor 2 (ER beta) (ESR2), transcript variant a | 10 ug |
$741.00
|
|
Citations
17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression
Huang, Zhang, Liao et al
J. Exp. Clin. Cancer Res. (2018) 37 (1), 133 DOI: 10.1186/s13046-018-0804-5
Huang, Zhang, Liao et al
J. Exp. Clin. Cancer Res. (2018) 37 (1), 133 DOI: 10.1186/s13046-018-0804-5
AI Summary: OriGene Technologies contributed to this study by providing the ERβ-expressing plasmid and its corresponding empty plasmid, which were used to investigate the role of ERβ in regulating IL6 expression and lung adenocarcinoma progression.
Snippet: 2 and 95% air. The expression vectors and Small interfering RNAs for ERβ were constructed as previously described [25]. Briefly, The ERβ-expressing plasmid and its empty plasmid were obtained from Origene Technologies Inc. (Rockville, USA). Small interfering RNAs targeting ERβ (ERβ-siRNA Stealth siRNAs HSS103378, Life Technologies, Carlsbad, CA) and control siRNAs (ctrl-siRNA, Life Technologi
Polyfluorinated iodine alkanes regulated distinct breast cancer cell progression through binding with estrogen receptor alpha or beta isoforms
Song, Liu, Sun et al
Environ. Pollut. (2018) 239, 300-307 DOI: 10.1016/j.envpol.2018.04.037
Song, Liu, Sun et al
Environ. Pollut. (2018) 239, 300-307 DOI: 10.1016/j.envpol.2018.04.037
AI Summary: OriGene contributed purified recombinant human ERα and ERβ proteins, which were used to study the binding affinities of polyfluorinated iodine alkanes to estrogen receptor isoforms in breast cancer cell models.
Snippet: The series of working solutions were freshly diluted with sterile cell exposure medium, and the final DMSO concentration was less than 0.5%. The purified recombinant human ERα and ERβ proteins were obtained from OriGene Technologies Inc
The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading
Tarallo, Giurato, Bruno et al
Genome Biol. (2017) 18 (1), 189 DOI: 10.1186/s13059-017-1321-0
Tarallo, Giurato, Bruno et al
Genome Biol. (2017) 18 (1), 189 DOI: 10.1186/s13059-017-1321-0
AI Summary: OriGene’s pCMV6-ESR2 (RC218519) human full-length cDNA clone was used to generate ERβ-tagged inducible clones, enabling the study of ERβ’s interactions and regulatory roles in gene transcription, RNA splicing, and RISC loading described in the paper.
Snippet: Skip to main content. Advertisement. We are working on a new version of this page and we'd like your opinion. See an early preview or learn more. Biomed Central ... Cached;For generation of ERβ tagged inducible clones, the human full-length cDNA clone pCMV6-ESR2 (RC218519) encoding human ESR2 was purchased from Origene
Synthesis and structure-activity relationships of 1-benzylindane derivatives as selective agonists for estrogen receptor beta
Yonekubo, Fushimi, Miyagi et al
Bioorg. Med. Chem. (2016)
Yonekubo, Fushimi, Miyagi et al
Bioorg. Med. Chem. (2016)
Cited by 17
AI Summary: OriGene products contributed to this study by providing the cDNA plasmids for human ERα and ERβ, which were used to construct expression vectors for evaluating the activity of synthesized 1-benzylindane derivatives.
Snippet: The expression vectors for human ERα and human ERβ were constructed by inserting the cDNA of ERs from plasmid (OriGene, Rockville, MD) into pcDNA3.1 vectors
The Phytoestrogen Genistein Affects Breast Cancer Cells Treatment Depending on the ERα/ERβ Ratio
Pons, Nadal-Serrano, Torrens-Mas et al
J. Cell. Biochem. (2015)
Pons, Nadal-Serrano, Torrens-Mas et al
J. Cell. Biochem. (2015)
Cited by 63
AI Summary: OriGene contributed to this study by providing the ERβ cDNA clone, which was used to stably transfect breast cancer cells and investigate the effects of genistein treatment in relation to the ERα/ERβ ratio.
Snippet: cells), and the combination of GEN with each cytotoxic agent for 48 hours. Estrogen receptor β stable transfection ERβ cDNA clone was purchased from Origene (Rockville, MD, USA). First of all, ERβ cDNA clone was amplified in ... Cite SaveSaving
Δ(9)-Tetrahydrocannabinol Disrupts Estrogen-Signaling through Up-Regulation of Estrogen Receptor β (ERβ)
Takeda, Yoshida, Nishimura et al
Chem. Res. Toxicol. (2013)
Takeda, Yoshida, Nishimura et al
Chem. Res. Toxicol. (2013)
Cited by 64
Showing 1-6 of 6 papers.
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