IL6 (NM_000600) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IL6 |
| Synonyms | BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000600 |
| ORF Size | 636 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000600.5 |
| RefSeq Size | 1201 bp |
| RefSeq ORF | 639 bp |
| Locus ID | 3569 |
| UniProt ID | P05231, Q75MH2, B4DVM1 |
| Cytogenetics | 7p15.3 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway |
| MW | 23.7 kDa |
| Summary | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). provided by RefSeq, Aug 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000600" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202078L1 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202078L2 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), mGFP tagged | 10 ug |
$750.00
|
|
| RC202078L3 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202078L4 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), mGFP tagged | 10 ug |
$750.00
|
|
| RG202078 | IL6 (tGFP-tagged) - Human interleukin 6 (interferon, beta 2) (IL6) | 10 ug |
$650.00
|
|
| SC125236 | IL6 (untagged)-Human interleukin 6 (interferon, beta 2) (IL6) | 10 ug |
$300.00
|
|
Citations
The p62-CD63 complex mediates amphisome biogenesis for secretory autophagy under oxidative stress
Ji, Kim, Lee et al
Research Square (2025)
Ji, Kim, Lee et al
Research Square (2025)
Interleukin-6 Response of C2C12 Myotubes Stimulated with Lipopolysaccharide and Lipoic Acid
Wu, Chen
J. Interferon Cytokine Res. (2020)
Wu, Chen
J. Interferon Cytokine Res. (2020)
AI Summary: OriGene contributed to this study by providing the pCMV6-IL-6 cDNA plasmid, which was used to overexpress IL-6 in C2C12 myotubes for investigating IL-6 signaling mechanisms.
Snippet: environment with 5% CO 2 . Transfection and treatment of lipoic acid, lipopolysaccharide, anti-IL-6Rα, and LY294002. pCMV6-IL-6 cDNA plasmid was purchased from Origene Technologies, Inc. (Rockville, MD). The C 2 C 12
Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation
Li, Zhao, Xu et al
Protein Cell (2018)
Li, Zhao, Xu et al
Protein Cell (2018)
Serine hydroxymethyl transferase 1 stimulates pro-oncogenic cytokine expression through sialic acid to promote ovarian cancer tumor growth and progression
Gupta, Yang, Dogra et al
Oncogene (2017)
Gupta, Yang, Dogra et al
Oncogene (2017)
AI Summary: OriGene products contributed to this paper by providing the SHMT1, IL-6, and IL-8 cDNAs, which were used for gene overexpression studies to investigate their roles in ovarian cancer growth and progression.
Snippet: All the cell lines were mycoplasma free and were authenticated by STR profiling. SHMT1, IL-6 and IL-8 cDNAs were purchased from Origene (Rockville, MD, USA). Lentiviral shRNA plasmids were obtained from Open Biosystems. ... All 3 versions Cite SaveSaving
DNA Methyl Transferase 1 Reduces Expression of SRD5A2 in the Aging Adult Prostate
Ge, Wang, Bechis et al
Am. J. Pathol. (2015) 185 (3), 870-82 DOI: 10.1016/j.ajpath.2014.11.020
Ge, Wang, Bechis et al
Am. J. Pathol. (2015) 185 (3), 870-82 DOI: 10.1016/j.ajpath.2014.11.020
Cited by 38
AI Summary: In this study, OriGene's IL-6 cDNA was used to investigate the role of inflammatory mediators in regulating DNA methyltransferase 1 expression and subsequent SRD5A2 promoter methylation in prostate cells.
Snippet: 6534). IL-6 cDNA was purchased from Origene (Rockville, MD);human DNMT1 cDNA was purchased from Genecopoeia (Rockville, MD);and pcDNA1-p65/ Rel A was a gift from Abdalla Jama (Karolinska Institute, Sweden). ... Cited by 1 Cite SaveSaving
Kaposi's sarcoma-associated herpesviral IL-6 and human IL-6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation
Kang, Majerciak, Uldrick et al
J. Pathol. (2011) 225 (3), 378-89 DOI: 10.1002/path.2962
Kang, Majerciak, Uldrick et al
J. Pathol. (2011) 225 (3), 378-89 DOI: 10.1002/path.2962
Cited by 72
AI Summary: OriGene's SC125236 plasmid containing human IL-6 (hIL-6, NM_000600) was used as a template for PCR amplification to study miRNA-mediated regulation of IL-6 expression in the research.
Snippet: Unlike hIL-6, vIL-6 binds to the signal transducer protein, gp130, without the need to bind to the hIL-6 receptor-α. ... NM_000 600) from the SC125236 plasmid (Origene, Rockville, MD, USA) was amplified by PCR with a primer pair of oJGK24 and oJGK25.
Showing 1-6 of 7 papers.
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