IL6 (NM_000600) Human Untagged Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | IL6 |
| Synonyms | BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000600 |
| Insert Size | 639 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000600.1, NP_000591.1 |
| RefSeq Size | 1125 bp |
| RefSeq ORF | 639 bp |
| Locus ID | 3569 |
| UniProt ID | P05231 |
| Cytogenetics | 7p15.3 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway |
| Summary | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). provided by RefSeq, Aug 2020 Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000600" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202078 | IL6 (Myc-DDK-tagged)-Human interleukin 6 (interferon, beta 2) (IL6) | 10 ug |
$450.00
|
|
| RC202078L1 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202078L2 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), mGFP tagged | 10 ug |
$750.00
|
|
| RC202078L3 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202078L4 | Lenti ORF clone of Human interleukin 6 (interferon, beta 2) (IL6), mGFP tagged | 10 ug |
$750.00
|
|
| RG202078 | IL6 (tGFP-tagged) - Human interleukin 6 (interferon, beta 2) (IL6) | 10 ug |
$650.00
|
|
Citations
Interleukin-8 Expression is Associated with Prostate Cancer Aggressiveness and Androgen Receptor Loss in Primary and Metastatic Prostate Cancer
Maynard, Ertunc, Kulac et al
Mol. Cancer Res. (2019) 18 (1), 153-165 DOI: 10.1158/1541-7786.MCR-19-0595
Maynard, Ertunc, Kulac et al
Mol. Cancer Res. (2019) 18 (1), 153-165 DOI: 10.1158/1541-7786.MCR-19-0595
A Paracrine Role for IL6 in Prostate Cancer Patients: Lack of Production by Primary or Metastatic Tumor Cells
Yu, Zheng, Esopi et al
Cancer Immunol Res (2015) 3 (10), 1175-84 DOI: 10.1158/2326-6066.CIR-15-0013
Yu, Zheng, Esopi et al
Cancer Immunol Res (2015) 3 (10), 1175-84 DOI: 10.1158/2326-6066.CIR-15-0013
AI Summary: In this study, OriGene products—including the IL6 cDNA clone (OriGene, SC1196) and IL6 qPCR primer set (OriGene, HP208773)—were used to validate and quantify IL6 mRNA expression, helping to demonstrate that IL6 is produced by stromal, not tumor, cells in prostate cancer tissues.
DNA Methyl Transferase 1 Reduces Expression of SRD5A2 in the Aging Adult Prostate
Ge, Wang, Bechis et al
Am. J. Pathol. (2015) 185 (3), 870-82 DOI: 10.1016/j.ajpath.2014.11.020
Ge, Wang, Bechis et al
Am. J. Pathol. (2015) 185 (3), 870-82 DOI: 10.1016/j.ajpath.2014.11.020
Cited by 38
AI Summary: In this study, OriGene's IL-6 cDNA was used to investigate the role of inflammatory mediators in regulating DNA methyltransferase 1 expression and subsequent SRD5A2 promoter methylation in prostate cells.
Snippet: 6534). IL-6 cDNA was purchased from Origene (Rockville, MD);human DNMT1 cDNA was purchased from Genecopoeia (Rockville, MD);and pcDNA1-p65/ Rel A was a gift from Abdalla Jama (Karolinska Institute, Sweden). ... Cited by 1 Cite SaveSaving
Establishment of a novel cell-based assay for screening small molecule antagonists of human interleukin-6 receptor
He, Yan, Zhang et al
Acta Pharmacol. Sin. (2014) 35 (11), 1453-62 DOI: 10.1038/aps.2014.90
He, Yan, Zhang et al
Acta Pharmacol. Sin. (2014) 35 (11), 1453-62 DOI: 10.1038/aps.2014.90
Cited by 2
AI Summary: OriGene products contributed to this study by providing the human IL-6 and IL-6Rα genes, which were essential for constructing the recombinant plasmids used to establish the novel cell-based assay for screening IL-6R antagonists.
Snippet: Materials and methods. Materials and instrumentation. The human IL-6 and IL-6Rα genes were purchased from Origene Technologies. The HEK293A cells, 7TD1 cells and U937 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai ... Cite SaveSaving
Kaposi's sarcoma-associated herpesviral IL-6 and human IL-6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation
Kang, Majerciak, Uldrick et al
J. Pathol. (2011) 225 (3), 378-89 DOI: 10.1002/path.2962
Kang, Majerciak, Uldrick et al
J. Pathol. (2011) 225 (3), 378-89 DOI: 10.1002/path.2962
Cited by 72
AI Summary: OriGene's SC125236 plasmid containing human IL-6 (hIL-6, NM_000600) was used as a template for PCR amplification to study miRNA-mediated regulation of IL-6 expression in the research.
Snippet: Unlike hIL-6, vIL-6 binds to the signal transducer protein, gp130, without the need to bind to the hIL-6 receptor-α. ... NM_000 600) from the SC125236 plasmid (Origene, Rockville, MD, USA) was amplified by PCR with a primer pair of oJGK24 and oJGK25.
Showing 1-5 of 5 papers.
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