Native Proteins

Streptavidin Peroxidase Conjugated

Fourier-transform infrared (FTIR) spectroscopy. Mean FTIR spectra of the five collagen types. Maximal intensity. Fig. 2. PMID: 19685340.

Streptavidin

Glycated collagen 1 regulates adipocyte insulin signaling and Rho and AGE scavenger receptor expression. Preadipocytes from human VAT were differentiated into mature adipocytes in 2D culture +/− unglycated or glycated recombinant human collagen 1 (UC1, GC1), then studied with glucose uptake assay or RT-qPCR. (a) Glucose uptake in non-stimulated (basal) or insulin-stimulated conditions. Ordinate: mean glucose uptake measured by 3H-2D-glucose in cell lysates (cpm) normalized to cell lysate protein concentration (mg/ml); bars with different letters indicate P 

TRAIL-mediated apoptosis of human plasma cells. Top, Control CESS cells (blast) or IL-6-differentiated plasma cells were cultured for 24 h in the presence or absence of 1.0 μg/ml TRAIL, DR5-Fc, or the Fc portion of human IgG. Cell viability was determined by trypan blue exclusion, and the percent of plasma cell death was calculated by dividing the number of dead plasma cells by the total number of plasma cells in the culture. Bottom, ELISA of IgG secretion in the corresponding culture medium. Data represent the relative IgG titer required to obtain a single OD value that lies within the linear range. Data represent three independent cell populations from one experiment. Three independent experiments were performed with similar results. Fig 3. PMID: 12421926.

Avidin Glucose Oxidase Conjugated

Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA. **P

IgM localizes within the conduit system of reactive LNs. (A) Confocal imaging of reactive and steady state auricular LNs from WT (C57BL/6J) or B cell–deficient (JHT) mice, 10 d after intradermal CFA immunization in the ears. Sections were stained for CD3, B220, collagen IV (col.IV), and IgM. Insets display high-magnification views of collagen IV+ conduits in the T cell zone. Bars, 100 µm (left panels); 10 µm (right panels). Data are representative of three experiments (two mice per condition and experiment). (B) Confocal imaging of auricular dLNs and contralateral ndLNs from Ccl19-Cre:RosatdT mice, 10 d after intradermal CFA injection in one ear. Sections were stained for collagen IV and IgM. tdT, tdTomato. Bars, 5 µm. Pictures are representative of three experiments (two mice per experiment). (C) Representative EM pictures of FRCs and conduits in steady state and auricular reactive LNs of WT and JHT mice, 10 d after intradermal CFA injection in one ear. Ultrathin sections were stained with 6-nm gold nanobeads specific for mouse IgM (highlighted with arrowheads), and the average number of beads located in the conduit lumen was evaluated for each condition. Bars, 2 µm (upper panels); 200 nm (lower panels). Data are representative of two experiments (one individual per condition and experiment). Results are expressed as mean ± SEM. *, P < 0.05; ****, P < 0.0001. Figure 1. PMID: 30429248.

SEPHAROSE™ Protein A

Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureus.

Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureus.

Biotin Alkaline Phosphatase Conjugated

Aggf1 interacts with and modulates SMAD7 activity. (D) Primary HSCs were infected with Ad-V or Ad-AGGF1 and then transfected with either siRNA targeting SMAD7 or scrambled siRNA (SCR). Expression of fibrogenic genes was examined by Western and qPCR (not shown). Figure 4. PMID: 26850475.

Biotin Peroxidase Conjugated

Streptavidin Rhodamine Conjugated

SDS-PAGE results of Mouse IgG Control Low Endotoxin. Lane 1: reduced Mouse IgG Control Low Endotoxin. Lane 2: opal prestained molecular weight ladder (p/n MB-210-0500). Lane 3: non-reduced Mouse IgG Control Low Endotoxin. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.

Streptavidin Phycoerythrin Conjugated

Avidin

SDS-PAGE of Bovine Collagen Type V. Lane 1: Bovine Collagen Type V Reduced (5.0µg). Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Bovine Collagen Type V Non-Reduced (5.0µg). Predicted MW: >95kDa. Observed MW: α1 and α2 bands. 4-20% Gel Coomassie Stained.

Published customer image: Purified Human CD66e (AR24002PU-N) used for the preparation of CD66e (CEA) standard solutions for the development of a CD66e functionalized nanoparticle based CD66e MLI. (magnetically labeled immunoassay). Image caption:Spiked-CEA-concentration-in-serum, фCEA, and (a) IMR signal and (b) the CEA concentration, фCEA-IMR, derived using the immunomagnetic reduction method.From: Yang CC, Yang SY, Ho CS, Chang JF, Liu BH, Huang KW. Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use.J Nanobiotechnology. 2014 Nov 26;12(1):44.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5µL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.

SDS-Page of Rat IgM Whole Molecule. Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 78 and 25 kDa.

Published customer image: Purified Rat IgE kappa (SA022) used for the generation of an IgE concentration standard curve by ELISA.Image caption:(A) IgE standard curve. (B) Assessment of the intra/inter-assay variability by reproducing the IgE immunoassay on various plates on the same day or on two separate days (1 week apart) with the selected IgE antibody (CV, coefficient of variation). mAbs414nm, absorbance unit at 414 nm. From: Tulinská J, Adel-Patient K, Bernard H, Líšková A, Kuricová M, Ilavská S, Horváthová M, Kebis A, Rollerová E, Babincová J, Alá&ccaron;ová R, Wal JM, Schmidt K, Schmidtke J, Schmidt P, Kohl C, Wilhelm R, Schiemann J, Steinberg P. Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days.(EU 7th Framework Programme project GRACE).Arch Toxicol. 2018 Jul;92(7):2385-99.doi: 10.1007/s00204-018-2230-z.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Streptavidin Fluorescein Conjugated

Sandwich ELISA analysis of human IgA binding using Mouse anti Human IgA (MCA476G) as a capture reagent and biotinylated Mouse anti Human IgA (5104-3104) as a detection reagent with purified human IgA (BA325) as antigen. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum sample is displayed at 25000 (Blue) and 50000 (Red) fold dilutions.