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Streptavidin Protein

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SKU
RA021F
Streptavidin protein, 1 mg
Size:
1 mg
  $400.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Protein Source S. avidinii
Concentration lot specific
Purity : Prepared from Chromatographically pure Streptavidin.
Conjugation FITC
Buffer State: Lyophilized purifed
Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 Da).
Absorption/Emission Wavelength: 495 nm/528 nm
Fluorochrome/Protein Ratio: 2.2 moles FITC per mole of Streptavidin.
Presentation Label: FITC
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Preparation Lyophilized purifed
Recommended Dilution Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Protein Description Fluorescein Conjugated Streptavidin
Note Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.
Storage Store vial at 2-8°C prior to restoration.
For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
Synonyms StAv
Summary Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Protein Families 000-1/5, 000-1/50, 000.
Flow Cytometry: 1/500-1/2, 000.
IF Microscopy: 1/1, 500., Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays.
Recommended Dilutions:
FLISA: 1/10
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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