Streptavidin Protein

SKU RA021F

Streptavidin protein, 1 mg

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: : Prepared from Chromatographically pure Streptavidin.
• Conjugation: FITC
• Buffer: State: Lyophilized purifed; Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.; Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 Da).; Absorption/Emission Wavelength: 495 nm/528 nm; Fluorochrome/Protein Ratio: 2.2 moles FITC per mole of Streptavidin.; Presentation Label: FITC
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purifed
• Recommended Dilution: Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays.; Recommended Dilutions:; FLISA: 1/10,000-1/50,000.; IF Microscopy: 1/1,000-1/5,000.; Flow Cytometry: 1/500-1/2,500.
• Protein Description: Fluorescein Conjugated Streptavidin
• Note: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.
• Storage: Store vial at 2-8°C prior to restoration.; For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.; Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• Synonyms: StAv
• Summary: Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
• Protein Families: 000-1/5, 000-1/50, 000.<br />Flow Cytometry: 1/500-1/2, 000.<br />IF Microscopy: 1/1, 500., Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays.<br /><u>Recommended Dilutions:</u><br />FLISA: 1/10