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Streptavidin Protein

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SKU
RA021HRP
Streptavidin protein, 1 mg
Size:
1 mg
  $445.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Protein Source S. avidinii
Concentration lot specific
Purity Prepared from Chromatographically purified streptavidin and labelled with Horseradish Peroxidase (HRP).
Conjugation HRP
Buffer State: Lyophilized purifed protein.
Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Label: Horseradish Peroxidase (HRP)
Preservative: 0.01% (w/v) Gentamicin sulfate
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Presentation Label: HRP
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Preparation Lyophilized purifed protein.
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot, 1/10,000-1/40,000), ELISA (1/20,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-biotin-avidin based enzymatic assays.
Note Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

Caution: Do Not Add Sodium Azide
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
Synonyms StAv
Summary Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Horse Raddish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Protein Families 000), 000) as well as other peroxidase-biotin-avidin based enzymatic assays., 000-1/200, 000-1/40, 000-1/5, 1/10, ELISA (1/20, Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/1, Suitable for Immunoblotting (Western or Dot blot
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Documents
Resources

file-alt Citations

miR-30a targets STOX2 to increase cell proliferation and metastasis in hydatidiform moles via ERK, AKT, and P38 signaling pathways
Guo, Zhu, Wang et al
Cancer Cell Int (2022) 22 (1), 103 DOI: 10.1186/s12935-022-02503-3
Cited by 2
AI Summary: OriGene’s 3,3'-diaminobenzidine (DAB) staining reagent was used in this study to visualize tissue sections during immunohistochemistry, contributing to the detection of protein expression related to miR-30a and STOX2 in hydatidiform mole samples.
Snippet: , Elabscience). Next, the secondary antibody and horseradish peroxidase streptavidin were incubated for 30 min at 37 °C, respectively. The sections were stained using 3, 3'-diaminobenzidine (DAB) (OriGene Technologies, Beijing, China) and hematoxylin (KeyGEN BioTECH, Jiangsu, China). Tissues sections were imaged under a light microscope. Hematoxylin and eosin (HE) staining The Hydatidiform mol
FigureFigureFigure
Showing 1-1 of 1 papers.
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