Mouse IgG (Fc specific) Mouse Protein
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Protein Source | Serum |
| Concentration | 1.027 mg/mL by UV absorbance at 280 nm |
| Purity | MOUSE IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c). No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain. |
| Presentation State | Purified |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preparation | Lyophilized purified protein. |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Mouse IgG F(c) fragment Purified. |
| Note | Mouse IgG F(c) Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | MOUSE IgG F(c) fragment, Mouse Fc, Immunoglobulin Fc |
| Summary | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins. |
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