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Rat IgM Rat Protein

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SKU
RA050
Rat IgM
Size:
1 mg
  $495.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Rat
Protein Source Serum
Concentration 1.1 mg/mL by UV absorbance at 280 nm
Purity Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (µ chain specific).   No reaction was observed against anti-Rat IgG F(c).  Some light chain cross-reactivity will occur with anti-Rat IgG.
Buffer 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preparation Liquid (sterile filtered) purified Ig fraction.
Recommended Dilution ELISA,Immunohistochemistry,Western Blot
Note Rat IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Storage Store vial at 4° C prior to opening.   Rat IgM whole molecule is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms Rat Immunoglobulin M
Summary Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Protein Families as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins., Suitable for use as antigen or ligand in immunochemical reactions
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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