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Mouse IgM Mouse Protein

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SKU
RA048
Mouse IgM
Size:
1 mg
  $500.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Mouse
Protein Source Serum
Concentration 1.1 mg/ml by UV absorbance at 280 nm
Purity Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (µ chain specific).   No reaction was observed against anti-Mouse IgG F(c).  Some light chain cross-reactivity will occur with anti-Mouse IgG.
Buffer 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preparation Liquid (sterile filtered) Ig fraction.
Recommended Dilution ELISA,Western Blot
Protein Description Purified polyclonal Mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (µ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross reactivity will occur with anti-Mouse IgG.
Note Mouse IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Storage Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms Mouse Immunoglobulin M
Summary Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. IgM is predominantly produced in the spleen. Mouse IgM is formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites.
Protein Families as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins., Suitable for use as antigen or ligand in Immunochemical Reactions
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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