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Streptavidin Protein

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SKU
RA021PE
Streptavidin protein, 1 ml
Size:
1 ml
  $580.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Protein Source S. avidinii
Concentration lot specific
Purity Prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatographies.
Conjugation PE
Buffer State: Lyophilized purified Ig fraction
Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml BSA as a stabilizer and 0.01% Sodium Azide as a preservative
Label: R-Phycoerythrin (R-PE) (MW 240, 000 daltons).
Absorption/Emission Wavelength: 488 nm/575 nm
OD566 nm/OD280 nm: 2.5
Presentation Label: PE
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Preparation Lyophilized purified Ig fraction
Recommended Dilution This reagent has been specifically designed for the detection of biotinylated biological probes such as antibodies or cytokines in Flow Cytometry or Immunhistochemisty.
The maximum amount of reagent to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of Streptavdin RPE. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100-1/250 should be suitable for most applications.
Recommended Dilutions:
Flow Cytometry: 1/100-1/250.
IF Microscopy: 1/100-1/250
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
Synonyms StAv
Summary Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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