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Biotin (AP conjugated)

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SKU
RA104AP
Biotin conjugated with Alkaline Phosphatase, 1 mg
Size:
1 mg
  $310.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Concentration lot specific
Purity This product was prepared from Electrophoretically pure Alkaline Phosphatase and Biotin.
Conjugation AP
Buffer State: Liquid (sterile filtered) Ig fraction
Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 0.05% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Label: Alkaline Phosphatase (Calf Intestine)
Presentation Label: AP
Preparation Liquid (sterile filtered) Ig fraction
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot, 1/500-1/2,500), ELISA (1/8,000-1/32,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.
Protein Description Biotin Alkaline Phosphatase Conjugated .
Storage Store vial at 2-8°C before opening.
DO NOT FREEZE!
This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
Synonyms Vitamin B7, Vitamin H
Summary Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Protein Families 000) and Immunohistochemistry (1/200-1/1, 000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin., 000-1/32, 1/500-1/2, 500), ELISA (1/8, Suitable for Immunoblotting (Western or Dot blot
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Documents
Resources

file-alt Citations

CXCL13 shapes immunoactive tumor microenvironment and enhances the efficacy of PD-1 checkpoint blockade in high-grade serous ovarian cancer
Yang, Lu, Zhang et al
J Immunother Cancer (2021) 9 (1) DOI: 10.1136/jitc-2020-001136
Cited by 242
AI Summary: OriGene's Biotin-streptavidin Horseradish Peroxidase (HRP) detection system was used in this study to visualize and validate the presence of CXCL13 and CXCR5 in tissue sections via immunohistochemistry, aiding in the analysis of tumor microenvironment components.
Snippet: Biotin-streptavidinHorseradish Peroxidase (HRP) detection systems (Origene) were applied for the following steps. Sections of lymph nodes that were previously shown to be positive for CXCL13 and CXCR5 were used as positive controls;- Biotin-streptavidinHorseradish Peroxidase (HRP) detection systems (Origene) were applied for the following steps. Sections of lymph nodes that were previously show...
FigureFigureFigure
Cilostazol Induces PGI2 Production via Activation of the Downstream Epac-1/Rap1 Signaling Cascade to Increase Intracellular Calcium by PLCε and to Activate p44/42 MAPK in Human Aortic Endothelial Cells
Hashimoto, Tanaka, Takeda et al
PLoS ONE (2015) 10 (7), e0132835 DOI: 10.1371/journal.pone.0132835
Cited by 27
AI Summary: OriGene Technologies contributed to the paper by providing human recombinant PI3Kγ protein, which was used in Biacore analysis to investigate the signaling mechanisms underlying cilostazol-induced PGI2 synthesis in human aortic endothelial cells.
Snippet: siRNA against Epac-1 (sc-41700), Rap-1 (sc-38554), and PLCε (sc-44024) were purchased from Santa Cruz Biotechnology, Inc. For Biacore analysis, human recombinant PI3Kγ protein was from OriGene Technologies, Inc. ... Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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