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Protein A (Sepharose) Staphylococcus aureus Protein

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SKU
RA100S5
Sepharose™ Protein A
Size:
5 ml
  $770.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Staphylococcus aureus
Concentration 6.0 mg/cc drained sepharose gel (~1 cc settled sepharose beads per 2 ml suspension)
Purity Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose™ Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Buffer 20% (v/v) Ethanol
Bioactivity Specific: ~35 mg Human IgG bound per cc of drained beads.
Preparation Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads).
Recommended Dilution Immunoprecipitation
Protein Description Native Protein A (Staphylococcus aureus) coupled to bead by cyanogen bromide activation method.
Note Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
Storage Store SEPHAROSE PROTEIN A at 4° C prior to opening. DO NOT FREEZE.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms ProA, Staphylococcus A protein, Sepharose Protein A
Summary Sepharose™ Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureus. When evenly dispersed approximately 10 mL of suspension is equal to 5.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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