Collagen type VI Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Protein Source | Placenta |
| Concentration | 1.13 mg/mL by nanodrop at 205 nm |
| Purity | Human Collagen type VI has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with Rockland’s anti-Collagen I, II, III, IV or V is negligible. |
| Buffer | 0.5 M Acetic Acid |
| Preparation | Liquid (sterile filtered) fraction. |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Collagen type VI, prepared from human placenta, is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA). |
| Note | Human Collagen type VI purified protein standard has been tested in SDS-PAGE and is used as a control in ELISA and Western blot. Collagen type VI is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure. |
| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is six (6) months from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_001839 |
| Locus ID | 1291 |
| Cytogenetics | 21q22.3 |
| Synonyms | Type VI collagen, collagen 6, human collagen, collagen VI |
| Summary | The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. provided by RefSeq, Jul 2008 |
| Protein Categories | Growth Factors, Intracellular Proteins, Secreated Proteins |
| Protein Families | for immunoprecipitation and for Wwestern blotting., For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
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