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Biotin (HRP conjugated)

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SKU
RA104HRP
Biotin conjugated with HRP, 5 mg
Size:
5 mg
  $340.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Concentration lot specific
Purity This product was prepared from chromatographically purified biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.
Conjugation HRP
Buffer State: Lyophilized Biotin conjugated to Horderadish peroxidase (HRP)
Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA, IgG and Protease free as stabilizer and 0.01% (w/v) gentamicin sulfate.
Presentation Label: HRP
Reconstitution Method Restore with 5.0 ml of deionized water (or equivalent).
Do not add sodium azide!
Preparation Lyophilized Biotin conjugated to Horderadish peroxidase (HRP)
Recommended Dilution Suitable for immunoblotting (Western: 1/10,000-1/40,000 or dot blot), ELISA (1/20,000-1/200,000), immunoperoxidase electron microscopy and immunochemistry (1/1,000-1/5,000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays.
Storage Store vial at 4°C prior to restoration.
Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Reference Data
Synonyms Vitamin B7, Vitamin H
Summary Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Protein Families 000 or dot blot), 000), 000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays., 000-1/200, 000-1/40, 000-1/5, ELISA (1/20, immunoperoxidase electron microscopy and immunochemistry (1/1, Suitable for immunoblotting (Western: 1/10
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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