MolBio Tools for COVID-19 Research (SARS-CoV-2 infection)

COVID19 is caused by a newly identified coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early studies have shown SARS-CoV-2 attaches to the human cells through an interaction between viral “Spike” protein and human ACE2 receptors; after binding to the cell surface, the “Spike” protein gets cleaved by human TMPRSS2 protease to facilitate the entry of virus.

To help researchers further dissect the molecular mechanism of this new virus, OriGene has developed various MolBio products covering all the SARS-CoV-2 virus genes and many human genes that play important roles in the pathogenesis of the virus.

Gene centric MolBio tools for COVID-19 research including clones, CRISPR kits, qPCR reagents, RNAi for SARS-CoV-2 & human genes Gene centric MolBio tools for COVID-19 research

Receptor & Activator


ACE2 TMPRSS2 CD147 (BSG) Furin MASP2

SARS-CoV-2 Virus Genes

SARS-CoV-2 is an RNA virus containing ~30,000 nucleotides, which encodes ~29 viral proteins. These proteins carry out a range of jobs from virus replication to the virus infection.

The first gene (ORF1ab) of SARS-CoV-2 encodes many non-structural proteins (nsps), which play important roles in the replication and transcription of the virus. Downstream of ORF1ab are the genes for the four major structural proteins (S, M, E, and N proteins) and some accessory proteins. The structural proteins build up the structure of the virus as their names state. The full SARS-CoV-2 genome and the proteins it encodes are shown below.

SARS-CoV-2 genome contains many protein coding genes including the major structural proteins (S, M, E and N), nsps and accessory proteins

OriGene offers the complete set of pre-made ORF clones for the SARS-CoV-2 virus genes. They are cloned in different expression vectors with different tags. Some ORF sequences are codon optimized sequences specifically for the human cell expression. Some contain native virus gene sequences. You can also request a custom viral ORF clone if you want to switch the ORF into a different vector, or want to have a different viral gene sequence (mutant or fragment).

SARS-CoV-2 Virus Expression ORF Clones
(codon optimized for human protein expression)
Protein Catalog# Product Description
N
nucleocapsid
phosphoprotein
VC102563 Codon optimized SARS-CoV-2 N gene with a C-terminal Myc-DDK tag
S
surface
glycoprotein
VC102557 Codon optimized SARS-CoV-2 S gene with a C-terminal Myc-DDK tag
E
Envelop Protein
VC102565 Codon optimized SARS-CoV-2 E gene with a C-terminal Myc-DDK tag
M
Membrane
Glycoprotein
VC102559 Codon optimized SARS-CoV-2 M gene with a C-terminal Myc-DDK tag
ORF3a VC102558 Codon optimized SARS-CoV-2 ORF3a gene with a C-terminal Myc-DDK tag
ORF6 VC102560 Codon optimized SARS-CoV-2 ORF6 gene with a C-terminal Myc-DDK tag
ORF7a VC102561 Codon optimized SARS-CoV-2 ORF7a gene with a C-terminal Myc-DDK tag
ORF8 VC102562 Codon optimized SARS-CoV-2 ORF8 gene with a C-terminal Myc-DDK tag
ORF10 VC102564 Codon optimized SARS-CoV-2 ORF10 gene with a C-terminal Myc-DDK tag
SARS-CoV-2 Virus Expression ORF Clones
(Native Virus Gene Sequence)
Protein Catalog# Product Description
N
nucleocapsid
phosphoprotein
VC102555 Native SARS-CoV-2 N gene with a C-terminal Myc-DDK tag
S
surface
glycoprotein
VC102556 Native SARS-CoV-2 S gene extracellular domain with a C-terminal Myc-DDK tag
VC102566 Native SARS-CoV-2 S gene with a C-terminal Myc-DDK tag
E
Envelop Protein
VC102573 Native SARS-CoV-2 E gene with a C-terminal Myc-DDK tag
M
Membrane
Glycoprotein
VC102568 Native SARS-CoV-2 M gene with a C-terminal Myc-DDK tag
VC102583 Native SARS-CoV-2 M gene with a C-terminal DDK-RFP tag
ORF3a VC102567 Native SARS-CoV-2 ORF3a gene with a C-terminal Myc-DDK tag
VC102582 Native SARS-CoV-2 ORF3a gene with a C-terminal DDK-RFP tag
ORF6 VC102569 Native SARS-CoV-2 ORF6 gene with a C-terminal Myc-DDK tag
ORF7a VC102570 Native SARS-CoV-2 ORF7a gene with a C-terminal Myc-DDK tag
ORF8 VC102571 Native SARS-CoV-2 ORF8 gene with a C-terminal Myc-DDK tag
VC102584 Native SARS-CoV-2 ORF9b gene with a C-terminal DDK-RFP tag
VC102585 Native SARS-CoV-2 ORF9c gene with a C-terminal DDK-RFP tag
ORF10 VC102572 Native SARS-CoV-2 ORF10 gene with a C-terminal Myc-DDK tag
VC102586 Native SARS-CoV-2 ORF10 gene with a C-terminal DDK RFP tag
Nsp4 VC102574 Native SARS-CoV-2 Nsp4 gene with a C-terminal DDK-RFP tag
Nsp5 VC102575 Native SARS-CoV-2 Nsp5 gene with a C-terminal DDK-RFP tag
Nsp6 VC102576 Native SARS-CoV-2 Nsp6 gene with a C-terminal DDK-RFP tag
Nsp7 VC102577 Native SARS-CoV-2 Nsp7 gene with a C-terminal DDK-RFP tag
Nsp8 VC102578 Native SARS-CoV-2 Nsp8 gene with a C-terminal DDK-RFP tag
Nsp9 VC102579 Native SARS-CoV-2 Nsp9 gene with a C-terminal DDK-RFP tag
Nsp10 VC102580 Native SARS-CoV-2 Nsp10 gene with a C-terminal DDK-RFP tag
Nsp14 VC102581 Native SARS-CoV-2 Nsp14 gene with a C-terminal DDK-RFP tag
SARS-CoV-2 Virus DNA Template In PUC Vector
Protein Catalog# Product Description
N VC202563 Contains native ORF sequence for SARS-CoV-2 Nucleocapsid Phosphoprotein gene
S VC202557 Contains native ORF sequence for SARS-CoV-2 surface glycoprotein gene
M VC202559 Contains native ORF sequence for SARS-CoV-2 Membrane Glycoprotein gene
E VC202565 Contains native ORF sequence for SARS-CoV-2 Envelop Protein gene
ORF3a VC202558 Contains native ORF sequence for SARS-CoV-2 ORF3a gene
ORF6 VC202560 Contains native ORF sequence for SARS-CoV-2 ORF6 gene
ORF7a VC202561 Contains native ORF sequence for SARS-CoV-2 ORF7a gene
ORF8 VC202562 Contains native ORF sequence for SARS-CoV-2 ORF8 gene
ORF10 VC202564 Contains native ORF sequence for SARS-CoV-2 ORF10 gene

qPCR is the gold standard for gene expression analysis. It is a critical part of OriGene’s “gene-centric” product strategy. Our qSTAR qPCR primer pairs are designed for SYBR Green-based real-time qPCR. The primers are meticulously designed using OriGene's proprietary primer design algorithm developed from > 10,000 qPCR experiments. We are now offering the pre-validated qPCR primers and template standards for SARS-CoV-2 RdRP, N and S genes (below).

Relative positions of amplicon targets on SARS-CoV-2 (previous Wuhan-CoV) genome for virus RdRp, N and S genes. Relative positions of amplicon targets on SARS-CoV-2 (previous Wuhan-CoV) genome. ORF: open reading frame; RdRp: RNA-dependent RNA polymerase; S: surface glycoprotein; N: nucleocapsid. Numbers below amplicon are genome locations corresponding to Wuhan-CoV genome, MN908947.

SARS-CoV-2 Virus qPCR Quantification
Protein Catalog# Product Description
N HP234774 qSTAR qPCR primer pairs against SARS-CoV-2 N protein gene
HK212669 qPCR primer pairs and template standards against SARS-CoV-2 N protein gene
S HP234775 qSTAR qPCR primer pairs against SARS-CoV-2 S protein gene
HK212670 qPCR primer pairs and template standards against SARS-CoV-2 S protein gene
RdRp HP234776 qSTAR qPCR primer pairs against SARS-CoV-2 RdRp protein gene
HK212671 qPCR primer pairs and template standards against SARS-CoV-2 RdRp protein gene


ACE2 Receptor

Angiotensin-converting enzyme 2 (ACE2) is a membrane protein that has been found to play an important role in the infection of SARS-CoV-2 virus. The protein contains 805 amino acids with a N-terminal signal peptide, a transmembrane domain and a cytoplasmic domain. It is expressed in many tissues of human body, which may address the reason of non-respiratory symptoms seen in some COVID-19 patients . To many researchers, ACE2 has become a hot target due to the recent outbreak of COVID-19.

Human ACE2 MolBio Toolset
Application Catalog# Product Description
Overexpress ACE2
proteinin Mammalian
cells
SC124099 Untagged cDNA clone encodes native ACE2 protein.
RC208442 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
RG208442 ORF clone encodes C-terminal tGFP tagged ACE2 protein
RC208442L1 Lenti ORF clone with C-terminal Myc-DDK tag
RC208442L2 Lenti ORF clone with C-terminal mGFP tag
RC208442L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RC208442L4 Lenti ORF clone with mGFP tag and puro selection marker
RC208442L1V Lentiviral particle made from RC208442L1
RC208442L2V Lentiviral particle made from RC208442L2
RC208442L3V Lentiviral particle made from RC208442L3
RC208442L4V Lentiviral particle made from RC208442L4
Knockdown
ACE2 expression at
mRNA level
TR306881 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL306881 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL306881V Lentiviral shRNA particles made from TL306881
SR311790 siRNA to knockdown the ACE2 expression
Genome Editing KN408442 CRISPR gene knockout kit for human ACE2
GA111814 CRISPR gene activation kit for human ACE2
qPCR Quantification HP214223 qPCR primer pair for quantification of human ACE2 cDNA level
HK212668 qPCR template standard for the calculation of exact copy number
Mouse ACE2 MolBio Toolset
Application Catalog# Product Description
Overexpress ACE2
proteinin Mammalian
cells
MC221763 Untagged cDNA clone encodes native ACE2 protein.
MR210755 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
MG210755 ORF clone encodes C-terminal tGFP tagged ACE2 protein
MR210755L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
MR210755L4 Lenti ORF clone with mGFP tag and puro selection marker
MR210755L3V Lentiviral particle made from MR210755L3
MR210755L4V Lentiviral particle made from MR210755L4
Knockdown
ACE2 expression at
mRNA level
TR504379 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL504379 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL504379V Lentiviral shRNA particles made from TL504379
SR420297 siRNA to knockdown the ACE2 expression
Genome Editing KN500711 CRISPR gene knockout kit for mouse ACE2
GA208393 CRISPR gene activation kit for mouse ACE2
qPCR Quantification MP220690 qPCR primer pair for quantification of mouse ACE2 cDNA level
Rat ACE2 MolBio Toolset
Application Catalog# Product Description
Overexpress ACE2
proteinin Mammalian
cells
RN214151 Untagged cDNA clone encodes native ACE2 protein.
RR214151 ORF clone encodes C-terminal Myc-DDK tagged ACE2 protein
RR214151L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RR214151L4 Lenti ORF clone with mGFP tag and puro selection marker
RR214151L3V Lentiviral particle made from RR214151L3
RR214151L4V Lentiviral particle made from RR214151L4
Knockdown
ACE2 expression at
mRNA level
TR701518 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL701518 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL701518V Lentiviral shRNA particles made from TL701518
SR513861 siRNA to knockdown the ACE2 expression

TMPRSS2 protease

Many coronaviruses including HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus) are found to use the same protease TMPRSS2 for the entry of the virus. TMPRSS2 is a serine protease that can cleave and activate the S-protein of the virus, which leads to the entry of the virus. Two spliced variants encoding different isoforms have been found for this gene.

Human TMPRSS2 Variant 1 MolBio Toolset
Application Catalog# Product Description
Overexpress TMPRSS2
isoform 1 protein (long
isoform) in Mammalian
cells
SC327067 Untagged cDNA clone encodes native TMPRSS2 isoform 1 protein
RC228432 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 1 protein
RG228432 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 1 protein
RC228432L1 Lenti ORF clone with C-terminal Myc-DDK tag
RC228432L2 Lenti ORF clone with C-terminal mGFP tag
RC228432L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RC228432L4 Lenti ORF clone with mGFP tag and puro selection marker
RC228432L1V Lentiviral particle made from RC228432L1
RC228432L2V Lentiviral particle made from RC228432L2
RC228432L3V Lentiviral particle made from RC228432L3
RC228432L4V Lentiviral particle made from RC228432L4
3’ UTR Clone
miRNA regulation
SC215536 3’UTR clone to study the miRNA regulation of TMPRSS2 expression
Knockdown
TMPRSS2 expression
at mRNA level (work for both isoforms)
TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL308745V Lentiviral shRNA particles made from TL308745
SR322062 siRNA to knockdown the TMPRSS2 expression
Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2
GA104914 CRISPR gene activation kit for human TMPRSS2
qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level
HK207474 qPCR template standard for the calculation of exact copy number
Human TMPRSS2 Variant 2 MolBio Toolset
Application Catalog# Product Description
Overexpress TMPRSS2
isoform 2 protein (short
isoform) in Mammalian
cells
SC323858 Untagged cDNA clone encodes native TMPRSS2 isoform 2 protein
RC208677 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 isoform 2 protein
RG208677 ORF clone encodes C-terminal tGFP tagged TMPRSS2 isoform 2 protein
RC208677L1 Lenti ORF clone with C-terminal Myc-DDK tag
RC208677L2 Lenti ORF clone with C-terminal mGFP tag
RC208677L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RC208677L4 Lenti ORF clone with mGFP tag and puro selection marker
RC208677L1V Lentiviral particle made from RC208677L1
RC208677L2V Lentiviral particle made from RC208677L2
RC208677L3V Lentiviral particle made from RC208677L3
RC208677L4V Lentiviral particle made from RC208677L4
3’ UTR Clone
miRNA regulation
SC215537 3’UTR clone to study the miRNA regulation of TMPRSS2 expression
Knockdown
TMPRSS2 expression
at mRNA level (work for both isoforms)
TR308745 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL308745 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL308745V Lentiviral shRNA particles made from TL308745
SR322062 siRNA to knockdown the TMPRSS2 expression
Genome Editing KN408677 CRISPR gene knockout kit for human TMPRSS2
GA104914 CRISPR gene activation kit for human TMPRSS2
qPCR Quantification HP208758 qPCR primer pair for quantification of human TMPRSS2 cDNA level
HK207474 qPCR template standard for the calculation of exact copy number
Mouse TMPRSS2 MolBio Toolset
Application Catalog# Product Description
Overexpress TMPRSS2
protein in Mammalian cells
MC204660 Untagged cDNA clone encodes native TMPRSS2 protein.
MR207852 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein
MG207852 ORF clone encodes C-terminal tGFP tagged TMPRSS2 protein
MR207852L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
MR207852L4 Lenti ORF clone with mGFP tag and puro selection marker
MR207852L3V Lentiviral particle made from MR207852L3
MR207852L4V Lentiviral particle made from MR207852L4
Knockdown
TMPRSS2 expression at
mRNA level
TR502817 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL502817 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL502817V Lentiviral shRNA particles made from TL502817
SR415788 siRNA to knockdown the TMPRSS2 expression
Genome Editing KN517940 CRISPR gene knockout kit for mouse TMPRSS2
qPCR Quantification MP217322 qPCR primer pair for quantification of mouse TMPRSS2 cDNA level
MK203882 qPCR template standard for the calculation of exact copy number
Rat TMPRSS2 MolBio Toolset
Application Catalog# Product Description
Overexpress TMPRSS2
protein in Mammalian cells
RN214298 Untagged cDNA clone encodes native TMPRSS2 protein.
RR214298 ORF clone encodes C-terminal Myc-DDK tagged TMPRSS2 protein
RR214298L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RR214298L4 Lenti ORF clone with mGFP tag and puro selection marker
RR214298L3V Lentiviral particle made from RR214298L3
RR214298L4V Lentiviral particle made from RR214298L4
Knockdown
TMPRSS2 expression at
mRNA level
TR712521 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL712521 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL712521V Lentiviral shRNA particles made from TL712521

CD147 (BSG) Receptor

CD147 , also known as Basigin or EMMPRIN, is a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. It has been identified as target for COVID-19 treatment. CD147 interacts with the Spike protein and provides a novel route for SARS-CoV-2 invasion. Many transcript variants have been found for this gene.

In human, BSG encodes four different variants through alternative splicing. Variant 1 is the longest variant with three Ig-like domains. Variant 2 is the most abundant and contains two Ig-like domains. Variant 3 and 4 each has a single Ig-like domain.

Human CD147 MolBio Toolset
Application Catalog# Product Description
Overexpress CD147
protein in Mammalian
cells
SC303059 Untagged cDNA clone with wild type CD147 (Variant 1)
SC126562 Untagged cDNA clone with wild type CD147 (Variant 2)
SC127184 Untagged cDNA clone with wild type CD147 (Variant 3)
SC312450 Untagged cDNA clone with wild type CD147 (Variant 4)
RC219464 ORF clone with C-terminal Myc-DDK tagged CD147 (Variant 1)
RC203894 ORF clone with C-terminal Myc-DDK tagged CD147 (Variant 2)
RC221374 ORF clone with C-terminal Myc-DDK tagged CD147 (Variant 3)
RC219418 ORF clone with C-terminal Myc-DDK tagged CD147 (Variant 4)
RG219464 ORF clone with C-terminal tGFP tagged CD147 (Variant 1)
RG203894 ORF clone with C-terminal tGFP tagged CD147 (Variant 2)
RG221374 ORF clone with C-terminal tGFP tagged CD147 (Variant 3)
RG219418 ORF clone with C-terminal tGFP tagged CD147 (Variant 4)
RC219464L1 / RC219464L1V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 1)
RC203894L1 / RC203894L1V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 2)
RC219464L2 / RC219464L2V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 1)
RC203894L2 / RC203894L2V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 2)
RC219464L3 / RC219464L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 1) and puro selection marker
RC203894L3 / RC203894L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 2) and puro selection marker
RC221374L3 / RC221374L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 3) and puro selection marker
RC219418L3 / RC219418L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged CD147 (Variant 4) and puro selection marker
RC219464L4 / RC219464L4V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 1) and puro selection marker
RC203894L4 / RC203894L4V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 2) and puro selection marker
RC221374L4 / RC221374L4V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 3) and puro selection marker
RC219418L4 / RC219418L4V Lenti ORF clone / particle with C-terminal mGFP tagged CD147 (Variant 4) and puro selection marker
3’UTR clone SC209708 3`UTR clone of CD147 Variant 1 for miRNA target validation
SC209707 3`UTR clone of CD147 Variant 2 for miRNA target validation
SC209705 3`UTR clone of CD147 Variant 4 for miRNA target validation
Knockdown
CD147 expression at
mRNA level
TR314429 Retroviral shRNA without reporter (4 shRNA & 1 scrambled control)
TG314429 Retroviral shRNA with GFP reporter (4 shRNA & 1 scrambled control)
TL314429 Lentivrial shRNA with GFP (4 shRNA & 1 scrambled control)
TL314429V Lentiviral shRNA particles made from TL314429
SR300476 siRNA to knockdown the CD147 expression
Genome Editing KN403894 CRISPR gene knockout kit for human CD147
GA100475 CRISPR gene activation kit for human CD147
qPCR Quantification HP205533 qPCR primer pair for quantification of human CD147 cDNA level
HK201144 qPCR template standard for the calculation of exact copy number of human CD147 cDNA

Mouse BSG has two variants. Variant 2 lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform 2 has a shorter N-terminus when compared to isoform 1.

Mouse CD147 MolBio Toolset
Application Catalog# Product Description
Overexpress CD147
protein in Mammalian
cells
MC200670 Untagged cDNA clone for mouse CD147
MR225905 ORF clone with C-terminal Myc-DDK tagged mouse CD147 (variant 1)
MR225906 ORF clone with C-terminal Myc-DDK tagged mouse CD147 (variant 2)
MR203655 ORF clone with C-terminal Myc-DDK tagged mouse CD147 from tumor sample
MG225905 ORF clone with C-terminal tGFP tagged mouse CD147 (variant 1)
MG225906 ORF clone with C-terminal tGFP tagged mouse CD147 (variant 2)
MG203655 ORF clone with C-terminal tGFP tagged mouse CD147 from tumor sample
MR225905L1 / MR225905L1V Lenti ORF clone / particle with C-terminal Myc-DDK tagged mouse CD147 (variant 1)
MR225905L2 / MR225905L2V Lenti ORF clone / particle with C-terminal mGFP tagged mouse CD147 (variant 1)
MR225905L3 / MR225905L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged mouse CD147 (variant 1) and Puro selection marker
MR225906L3 / MR225906L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged mouse CD147 (variant 2) and Puro selection marker
MR203655L3 / MR203655L3V Lenti ORF clone / particle with C-terminal Myc-DDK tagged mouse CD147 (from Tumor sample) and Puro selection marker
MR225905L4 / MR225905L4V Lenti ORF clone / particle with C-terminal mGFP tagged mouse CD147 (variant 1) and Puro selection marker
MR225906L4 / MR225906L4V Lenti ORF clone / particle with C-terminal mGFP tagged mouse CD147 (variant 2) and Puro selection marker
MR203655L4 / MR203655L4V Lenti ORF clone / particle with C-terminal mGFP tagged mouse CD147 (from Tumor sample) and Puro selection marker r
Knockdown
CD147 expression at
mRNA level
TR500225 Retroviral shRNA including 4 shRNA & 1 scrambled control
TG500225 Retroviral shRNA with GFP reporter including 4 shRNA & 1 scrambled control
TL500225 Lentivrial shRNA including 4 shRNA & 1 scrambled control
TL500225V Lentiviral shRNA particles made from TL500225
SR407847 siRNA to knockdown the CD147 expression
Genome Editing KN502283 CRISPR gene knockout kit for mouse CD147
GA200444 CRISPR gene activation kit for mouse CD147
qPCR Quantification MP201239 qPCR primer pair for quantification of mouse CD147 cDNA level
MK200434 qPCR primer pairs and template standards against Mus musculus gene CD147
Rat CD147 MolBio Toolset
Application Catalog# Product Description
Overexpress CD147
protein in Mammalian
cells
RN200443 Untagged cDNA clone with native CD147 ORF (variant 1)
RN200442 Untagged cDNA clone with native CD147 ORF (variant 2)
RR200443 ORF clone with C-terminal Myc-DDK tagged rat CD147 (variant 1)
RR200442 ORF clone with C-terminal Myc-DDK tagged rat CD147 (variant 2)
RR200443L3 / RR200443L3V Lenti ORF clone / particle with Myc-DDK tagged CD147 variant 1 and puro selection marker
RR200442L3 / RR200442L3V Lenti ORF clone / particle with Myc-DDK tagged CD147 variant 2 and puro selection marker
RR200443L4 / RR200443L4V Lenti ORF clone / particle with mGFP tagged CD147 variant 1 and puro selection marker
RR200442L4 / RR200442L4V Lenti ORF clone / particle with mGFP tagged CD147 variant 2 and puro selection marker
Knockdown
CD147 expression at
mRNA level
TR708145 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL708145 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL708145V Lentiviral shRNA particles made from TL708145
SR500213 siRNA to knockdown the CD147 expression

Furin Protease

Furin is a protease highly expressed in the lungs. SARS-CoV-2 Spike protein contains a potential Furin cleavage site, which doesn’t present in other beta coronaviruses including the most closely related SARS-CoV. Thus, Furin is an important target for COVID-19 research.

Human Furin MolBio Toolset
Application Catalog# Product Description
Overexpress Furin
protein in Mammalian
cells
SC118550 Untagged cDNA clone encodes native Furin protein.
RC204279 ORF clone encodes C-terminal Myc-DDK tagged Furin protein
RG204279 ORF clone encodes C-terminal tGFP tagged Furin protein
RC204279L1 Lenti ORF clone with C-terminal Myc-DDK tag
RC204279L2 Lenti ORF clone with C-terminal mGFP tag
RC204279L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RC204279L4 Lenti ORF clone with mGFP tag and puro selection marker
RC204279L1V Lentiviral particle made from RC204279L1
RC204279L2V Lentiviral particle made from RC204279L2
RC204279L3V Lentiviral particle made from RC204279L3
RC204279L4V Lentiviral particle made from RC204279L4
3’UTR clone SC215432 3`UTR clone of Furin for miRNA target validation
Knockdown
Furin expression at
mRNA level
TR312903 Retroviral shRNA without reporter (4 shRNA & 1 scrambled control)
TG312903 Retroviral shRNA with GFP reporter (4 shRNA & 1 scrambled control)
TL312903 Lentivrial shRNA with GFP (4 shRNA & 1 scrambled control)
TL312903V Lentiviral shRNA particles made from TL312903
SR321213 siRNA to knockdown the Furin expression
Genome Editing KN404279 CRISPR gene knockout kit for human Furin
GA103367 CRISPR gene activation kit for human Furin
qPCR Quantification HP206229 qPCR primer pair for quantification of human Furin cDNA level
HK203210 qPCR template standard for the calculation of exact copy number
Mouse Furin MolBio Toolset
Application Catalog# Product Description
Overexpress Furin
protein in Mammalian
cells
MC228946 Untagged cDNA clone for human Furin variant 1
MC202300 Untagged cDNA clone for human Furin variant 2
MR210693 ORF clone encodes C-terminal Myc-DDK tagged Furin protein
MG210693 ORF clone encodes C-terminal tGFP tagged Furin protein
MR210693L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
MR210693L4 Lenti ORF clone with mGFP tag and puro selection marker
MR210693L3V Lentiviral particle made from MR210693L3
MR210693L4V Lentiviral particle made from MR210693L4
Knockdown
Furin expression at
mRNA level
TR501591 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL501591 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL501591V Lentiviral shRNA particles made from TL501591
SR420195 siRNA to knockdown the Furin expression
Genome Editing KN506176 CRISPR gene knockout kit for mouse Furin
GA203126 CRISPR gene activation kit for mouse Furin
qPCR Quantification MP222020 qPCR primer pair for quantification of mouse Furin cDNA level
MK201832 qPCR primer pairs and template standards against Mus musculus gene Furin
Rat Furin MolBio Toolset
Application Catalog# Product Description
Overexpress Furin
protein in Mammalian
cells
RN211389 Untagged cDNA clone encodes native Furin protein.
RR211389 ORF clone encodes C-terminal Myc-DDK tagged Furin protein
RR211389L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RR211389L4 Lenti ORF clone with mGFP tag and puro selection marker
RR211389L3V Lentiviral particle made from RR211389L3
RR211389L4V Lentiviral particle made from RR211389L4
Knockdown
Furin expression at
mRNA level
TR710330 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL710330 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL710330V Lentiviral shRNA particles made from TL710330
SR511101 siRNA to knockdown the Furin expression

Mannose Associated Serine Protease 2 (MASP2)

MASP2 is a key protease involved in the activation of the complement system. Recent studies have shown MASP2 can interact with the coronavirus N protein, which might lead to an excessive immune response in the COVID-19 patients.

Human MASP2 has two different transcript variants. Variant 2, also called MAp19, differs in the 3' UTR and 3' coding region compared to variant 1, resulting in a frameshift and upstream stop codon. Isoform 2 has a unique C-terminus and lacks the serine protease catalytic domain, compared to isoform 1.

Human MASP2 MolBio Toolset
Application Catalog# Product Description
Overexpress MASP2
protein in Mammalian
cells
SC124235 Untagged cDNA clone with native MASP2 (Variant 1).
SC110196 Untagged cDNA clone with native MASP2 (Variant 2).
RC216665 ORF clone with C-terminal Myc-DDK tagged MASP2 (Variant 1)
RC218844 ORF clone with C-terminal Myc-DDK tagged MASP2 (Variant 2)
RG216665 ORF clone with C-terminal tGFP tagged MASP2 (Variant 1)
RG218844 ORF clone with C-terminal tGFP tagged MASP2 (Variant 2)
RC216665L1 / RC216665L1V Lenti ORF clone / particles with C-terminal Myc-DDK tagged MASP2 (Variant 1)
RC216665L2 / RC216665L2V Lenti ORF clone with C-terminal mGFP tagged MASP2 (Variant 1)
RC216665L3 / RC216665L3V Lenti ORF clone / particles with C-terminal Myc-DDK tagged MASP2 (Variant 1) and puro selection marker
RC218844L3 / RC218844L3V Lenti ORF clone / particles with C-terminal Myc-DDK tagged MASP2 (Variant 2) and puro selection marker
RC216665L4 / RC216665L4V Lenti ORF clone / particles with C-terminal mGFP tagged MASP2 (Variant 1) and puro selection marker
RC218844L4 / RC218844L4V Lenti ORF clone / particles with C-terminal mGFP tagged MASP2 (Variant 2) and puro selection marker
3’UTR clone SC205081 3`UTR clone of MASP2 (variant 1) for miRNA target validation
SC201553 3`UTR clone of MASP2 (variant 2) for miRNA target validation
Knockdown
MASP2 expression at
mRNA level
TR303337 Retroviral shRNA without reporter (4 shRNA & 1 scrambled control)
TL303337 Lentivrial shRNA with GFP (4 shRNA & 1 scrambled control)
TL303337V Lentiviral shRNA particles made from TL303337
SR307327 siRNA to knockdown the MASP2 expression
Genome Editing KN416665 CRISPR gene knockout kit for human MASP2
GA107365 CRISPR gene activation kit for human MASP2
qPCR Quantification HP209527 qPCR primer pair for quantification of human MASP2 cDNA level
HK211722 qPCR template standard for the calculation of exact copy number

Mouse MASP2 also has two variants. Variant 2 includes an alternate segment, compared to variant 1, that causes a frameshift and results in a shorter transcript. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1.

Mouse MASP2 MolBio Toolset
Application Catalog# Product Description
Overexpress MASP2
protein in Mammalian
cells
MC204276 Untagged cDNA clone for mouse MASP2 variant 1
MR210030 ORF clone with C-terminal Myc-DDK tagged MASP2 (Variant 1)
MR224675 ORF clone with C-terminal Myc-DDK tagged MASP2 (Variant 2)
MG210030 ORF clone with C-terminal tGFP tagged MASP2 (Variant 1)
MG224675 ORF clone with C-terminal tGFP tagged MASP2 (Variant 2)
MR210030L3 / MR210030L3V Lenti ORF clone / particles with C-terminal Myc-DDK tagged MASP2 (Variant 1) and puro selection marker
MR224675L3 / MR224675L3V Lenti ORF clone / particles with C-terminal Myc-DDK tagged MASP2 (Variant 2) and puro selection marker
MR210030L4 / MR210030L4V Lenti ORF clone / particles with C-terminal mGFP tagged MASP2 (Variant 1) and puro selection marker
MR224675L4 / MR224675L4V Lenti ORF clone / particles with C-terminal mGFP tagged MASP2 (Variant 2) and puro selection marker
Knockdown
MASP2 expression at
mRNA level
TR501305 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL501305 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL501305V Lentiviral shRNA particles made from TL501305
SR419124 siRNA to knockdown the MASP2 expression
Genome Editing KN509808 CRISPR gene knockout kit for mouse MASP2
GA202579 CRISPR gene activation kit for mouse MASP2
qPCR Quantification MP207796 CRISPR gene activation kit for mouse MASP2
MK203615 qPCR primer pairs and template standards against Mus musculus gene MASP2
Rat MASP2 MolBio Toolset
Application Catalog# Product Description
Overexpress MASP2
in Mammalian cells
RN213700 Untagged cDNA clone with native MASP2
RR213700 ORF clone with C-terminal Myc-DDK tagged MASP2
RR213700L3 Lenti ORF clone with Myc-DDK tag and puro selection marker
RR213700L4 Lenti ORF clone with mGFP tag and puro selection marker
RR213700L3V Lentiviral particle made from RR213700L3
RR213700L4V Lentiviral particle made from RR213700L4
Knockdown
MASP2 expression at
mRNA level
TR713285 Retroviral shRNA DNA including 4 shRNA & 1 scrambled control
TL713285 Lentivrial shRNA DNA including 4 shRNA & 1 scrambled control
TL713285V Lentiviral shRNA particles made from TL713285
SR513411 siRNA to knockdown the MASP2 expression