MASP2 (NM_006610) Human 3' UTR Clone

SKU SC205081

3' UTR clone of mannan-binding lectin serine peptidase 2 (MASP2) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: MASP2
• Synonyms: MAP-2; MAP19; MASP-2; MASP1P1; sMAP
• ACCN: NM_006610
• Insert Size: 392 bp
• Sequence Data:
  Insert Sequence

  >SC205081 3’UTR clone of NM_006610 ; The sequence shown below is from the reference sequence of NM_006610. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TGGATCGAGAACATAATTAGTGATTTTTAACTTGCGTGTCTGCAGTCAAGGATTCTTCATTTTTAGAAA; TGCCTGTGAAGACCTTGGCAGCGACGTGGCTCGAGAAGCATTCATCATTACTGTGGACATGGCAGTTGT; TGCTCCACCCAAAAAAACAGACTCCAGGTGAGGCTGCTGTCATTTCTCCACTTGCCAGTTTAATTCCAG; CCTTACCCATTGACTCAAGGGGACATAAACCACGAGAGTGACAGTCATCTTTGCCCACCCAGTGTAATG; TCACTGCTCAAATTACATTTCATTACCTTAAAAAGCCAGTCTCTTTTCATACTGGCTGTTGGCATTTCT; GTAAACTGCCTGTCCATGCTCTTTGTTTTTAAACTTGTTCTTATTGA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006610.4
• Locus ID: 10747
• Summary: This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]