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pMirTarget 3' UTR Assay Vector

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SKU
PS100062
pMirTarget, the cloning vector for all 3' UTR clones for miRNA target validation, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Features

pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3' UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity.

  • High sensitivity of Luciferase assay due to IRES-driven luciferase cassette
  • RFP as a reporter for transfection monitoring and normalization
  • Neomycin selection marker for stable cell establishment
  • Compatible with Precision shuttling system
  • Can serve as a control for target validation experiments.
  • 3' UTR Clones for MicroRNA target validation
  • LUC_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

In vivo inhibition of miR-125b-5p modulates monocyte trafficking through the CCR7 receptor and reduces atherosclerosis
Mallén, Rotllan, Griñán et al
Am J Physiol Cell Physiol (2025)
Cited by 1
AI Summary: OriGene products, specifically the pMirTarget control vector (Cat.#PS100062) and the pCCR7wt reporter construct containing the CCR7 3’UTR (Cat.#SC211596), were used to assess the regulation of CCR7 expression by miR-125b-5p in this study.
Snippet: e then transfected with 100 ng of329the following reporter constructs: control pMirTarget (Origene Technologies,330Cat.#PS100062, Rockville, MD, USA), pCCR7wt which included the 3’UTR of the331CCR7 mRNA (Origene Technologies, Cat.#SC211596, Rockville, MD, USA) and332pCCR7Δ in which the miR-125b predicted binding site on CCR7 had been dele
In vivo inhibition of miR-125b modulates monocyte trafficking through the CCR7 receptor and attenuates atherosclerosis
Mallén, Varela, Rotllan et al
medRxiv (2024)
Snippet: pMirTarget reporter constructs (PS100062, Origene Technologies, Maryland, USA): the p_CCR7.WT plasmid with the wild type 3′UTR region of CCR7 gene (#SC211596;Origene pCCR7–3′UTR CCR7 (NM_001838) Human 3′UTR Clone) and p_CCR7.Mut with the mutated miR-125b predicted binding site from CCR7-3′UTR (#SC2211596_CW306881;Origene pCCR7–3′UTR CCR7 (NM_001838) alone as reference controls or together with 40 nM mirVana hsa-miR-125b-5p miRNa mimic (#4464066-MC10148;Thermofisher Scientifics, Massachusetts USA) using 0.3 μL TransIT-X2 (#6004 MirusBio, Wisconsin USA) according to the manufacturer’s protocol. …
ZFP36-mediated mRNA decay regulates metabolism
Cicchetto, Jacobson, Sunshine et al
Cell Rep (2023) 42 (5), 112411 DOI: 10.1016/j.celrep.2023.112411
Cited by 43
AI Summary: In this study, OriGene products PCMV6-Kan/Neo-Mouse Eno2 (Cat# MC201508) and pMirTarget vector (Cat# PS100062) were used to investigate how ZFP36-mediated mRNA decay regulates metabolic enzyme expression and activity.
Snippet: Cellular metabolism is tightly regulated by growth factor signaling, which promotes metabolic rewiring to support growth and proliferation. While growth factor-induced Save;PCMV6-Kan/Neo-Mouse Eno2 Origene Cat# MC201508 pMirTarget vector Origene Cat# PS100062 CMV-ZFP36 This paper N/A Renilla Luciferase William Lowry (University of California, Los Angeles) Cinkornpumin et al., 2017
miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met
Huang, Huang, Chen et al
Biomedicines (2021) 9 (10) DOI: 10.3390/biomedicines9101371
Cited by 12
AI Summary: OriGene products, specifically the pMirTarget vector and the mutant c-Met 3′UTR luciferase plasmid (cat. no. PS100062), were used to construct reporter assays that demonstrated c-Met as a direct target of miRNA-148a in this study.
Snippet: AAACACACGUGACU-3′) (cat. no. CW306397) was cloned into the pMirTarget vector to construct the wild-type (WT) c-Met 3′UTR plasmid or the mutant c-Met 3′UTR luciferase plasmid (cat. no. PS100062;OriGene Technologies, Rockville, MD, USA). Cells (1 × 105) were seeded into 24-well plates for 1 day and cultured until the cells reached 70–90% confluence. Subsequently, cells were transfected wit
FigureFigureFigure
Knockdown of lncRNA NUTM2A‑AS1 inhibits lung adenocarcinoma cell viability by regulating the miR‑590‑5p/METTL3 axis
Wang, Zha, Wang
Oncol Lett (2021) 22 (5), 798 DOI: 10.3892/ol.2021.13059
Cited by 30
AI Summary: OriGene's pMIR-REPORT luciferase vector (cat. no. PS100062) was used to generate wild-type and mutant NUTM2A-AS1 3'UTR reporter constructs, enabling the study to confirm the direct interaction between NUTM2A-AS1 and miR-590-5p in lung adenocarcinoma cells.
Snippet: …no. PS100062;OriGene Technologies, Inc.) to generate the WT‑3'‑UTR NUTM2A‑AS1 and MUT‑3'‑UTR NUTM2A‑AS1 constructs, respectively. …
Anti-miR-518d-5p overcomes liver tumor cell death resistance through mitochondrial activity
Fernández-Tussy, Rodríguez-Agudo, Fernández-Ramos et al
Cell Death Dis (2021) 12 (6), 555 DOI: 10.1038/s41419-021-03827-0
Cited by 28
AI Summary: OriGene products, specifically the human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and the empty vector pMiRTarget (cat. number PS100062), were used in luciferase reporter assays to investigate the regulatory effects of miR-518d-5p on c-Jun expression in liver tumor cells.
Snippet: A minimum of three replicates was included per experiment. Luciferase reporter assays. Human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and empty vector pMiRTarget (cat. number PS100062) were purchased from OriGene (USA);Human c-Jun 3′UTR clone in pMirTarget (cat. number SC213791) and empty vector pMiRTarget (cat. number PS100062) were purchased from OriGene (USA)
Showing 1-6 of 16 papers.
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