MASP2 (NM_139208) Human Untagged Clone

SKU SC110196

MASP2 (untagged)-Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MASP2
• Synonyms: MAP-2; MAP19; MASP-2; MASP1P1; sMAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139208, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGGCTGCTGACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCCTTGGGCCCGAAGTGGCCTG; AACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGGGAGTATGCCAATGACCAGGAGCGGCGCTG; GACCCTGACTGCACCCCCCGGCTACCGCCTGCGCCTCTACTTCACCCACTTCGACCTGGAGCTCTCCCAC; CTCTGCGAGTACGACTTCGTCAAGCTGAGCTCGGGGGCCAAGGTGCTGGCCACGCTGTGCGGGCAGGAGA; GCACAGACACGGAGCGGGCCCCTGGCAAGGACACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTT; CCGCTCCGACTACTCCAACGAGAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAGGACATTGAC; GAGTGCCAGGTGGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCACAACCACCTGGGCGGTTTCT; ACTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAGCGCACCTGCTCAGAGCAGAGCCTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139208 unedited; TTATGTAATCCGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACCAGGCCAGCTGGAC; GGGCACACCATGAGGCTGCTGACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCC; TTGGGCCCGAAGTGGCCTGAACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGG; GAGTATGCCAATGACCAGGAGCGGCGCTGGACCCTGACTGCACCCCCCGGCTACCGCCTG; CGCCTCTACTTCACCCACTTCGACCTGGAGCTCTCCCACCTCTGCGAGTACGACTTCGTC; AAGCTGAGCTCGGGGGCCAAGGTGCTGGCCACGCTGTGCGGGCAGGAGAGCACAGACACG; GAGCGGGCCCCTGGCAAGGACACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTTC; CGCTCCGACTACTCCAACGAGAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAG; GACATTGACGAGTGCCAGGTGGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCAC; AACCACCTGNGCGGTTTCTACTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAG; CGCACCTGCTCAGAGCAGAGCCTCTAGCCTCCCCTGGAGCTCCGGCCTGCCCAGCAGGTC; AGAAGCCAGAGCCAGCCTGCTGGCCTCAGCTCCGGGTTGGGCTGAGATGGCTGTGCCCCA; ACTTCCCATTCACCCACCATGGACCCATAATTAACCTGGCCCCACCCCANTAAAAAAAAA; ANAAAAAACCCGACTCTAGATTGGCGCCCGCGTCATAGCTGTTTCCTGAAAAATCCCGGG; TGGCATCCCTTGAACCCCTCCCAGAGCCTTTCCTGCCCTGNAAGTTGCCTCCCGTGCCAC; CACCCTGNCCTATAAATTAGGTGCATCATTGTCTGACAGGGGCCTCCTAAATACGTGCGG; GGGCGCGGGGCCCGCCCTCTACCCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139208 unedited; NNAAAGATNTTTACTCTGNACCGCGGNCCCGCATTCNANGATCGAGTTTTTTTTTTTTTT; TTTTTTTGGGGTGGGGCCGGTTTATATTGGGTCCTGGGGGGTGAATGGGAGTTGGGGCAC; AGCCATCTCAGCCCAACCCGGAGCTGAGGCCAGCAGGCTGGCTCTGGCTTCTGACCTGCT; GGGCAGGCCGGAGCTCCAGGGGAGGCTAGAGGCTCTGCTCTGAGCAGGTGCGCTTGTTAC; GGTGCAAGACGTAGCCTGCGCGGCAGGAGCAGTAGAAACCGCCCAGGTGGTTGTGGCAGT; GGTGGTCGCAAGTGGGCGCCTCTCCCGGGGCCACCTGGCACTCGTCAATGTCCTCGGCTG; CATAGAAGGCCTCGAACCCCGTGAACGGCTTCTCGTTGGAGTAGTCGGAGCGGAAGGTAA; TGTCCAGGCTGGAGCCCAGCGAGTAGAAAGTGTCCTTGCCAGGGGCCCGCTCCGTGTCTG; TGCTCTCCTGCCCGCACAGCGTGGCCAGCACCTTGGCCCCCGAGCTCAGCTTGACGAAGT; CGTACTCGCAGAGGTGGGAGAGCTCCAGGTCGAAGTGGGTGAAGTAAAGGCGCAGGCGGT; AGCCGGGGGGTGCAGTCAGGGTCCAGCGCCGCTCCTGGTCATTGGCATACTCCCCTGGAA; AGCCGGGGGATGCCAGGCGCCCGACACAGGTTCAGGCCACTTCGGGCCCAAGGGGGGTGG; CCACCGAGCCACACAGAAGGCCCNAGNAGGTCAGCAGCCTCATGGTGTGCCCGTCCAGCT; GGCCTGGTGCCGANATCCGGCCGCCTATAGTGAGTCGTATACAAATTCTGACGGTCACTA; ACGAGCTCTGCTATATAGACTNCCACGTAACGCTACCGCCATTGCGTACGGGGCGGGGTA; TACGACATTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_139208
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139208.1, NP_631947.1
• RefSeq Size: 738 bp
• RefSeq ORF: 558 bp
• Locus ID: 10747
• UniProt ID: O00187
• Cytogenetics: 1p36.22
• Domains: CUB, EGF, EGF_CA
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Summary: This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (2), also called MAp19, differs in the 3' UTR and 3' coding region compared to variant 1, resulting in a frameshift and upstream stop codon. Isoform 2 has a unique C-terminus and lacks the serine protease catalytic domain, compared to isoform 1.