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MASP2 (NM_139208) Human Untagged Clone

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SKU
SC110196
MASP2 (untagged)-Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MASP2
Synonyms MAP-2; MAP19; MASP-2; MASP1P1; sMAP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_139208, the custom clone sequence may differ by one or more nucleotides


ATGAGGCTGCTGACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCCTTGGGCCCGAAGTGGCCTG
AACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGGGAGTATGCCAATGACCAGGAGCGGCGCTG
GACCCTGACTGCACCCCCCGGCTACCGCCTGCGCCTCTACTTCACCCACTTCGACCTGGAGCTCTCCCAC
CTCTGCGAGTACGACTTCGTCAAGCTGAGCTCGGGGGCCAAGGTGCTGGCCACGCTGTGCGGGCAGGAGA
GCACAGACACGGAGCGGGCCCCTGGCAAGGACACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTT
CCGCTCCGACTACTCCAACGAGAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAGGACATTGAC
GAGTGCCAGGTGGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCACAACCACCTGGGCGGTTTCT
ACTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAGCGCACCTGCTCAGAGCAGAGCCTCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_139208 unedited
TTATGTAATCCGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACCAGGCCAGCTGGAC
GGGCACACCATGAGGCTGCTGACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCC
TTGGGCCCGAAGTGGCCTGAACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGG
GAGTATGCCAATGACCAGGAGCGGCGCTGGACCCTGACTGCACCCCCCGGCTACCGCCTG
CGCCTCTACTTCACCCACTTCGACCTGGAGCTCTCCCACCTCTGCGAGTACGACTTCGTC
AAGCTGAGCTCGGGGGCCAAGGTGCTGGCCACGCTGTGCGGGCAGGAGAGCACAGACACG
GAGCGGGCCCCTGGCAAGGACACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTTC
CGCTCCGACTACTCCAACGAGAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAG
GACATTGACGAGTGCCAGGTGGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCAC
AACCACCTGNGCGGTTTCTACTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAG
CGCACCTGCTCAGAGCAGAGCCTCTAGCCTCCCCTGGAGCTCCGGCCTGCCCAGCAGGTC
AGAAGCCAGAGCCAGCCTGCTGGCCTCAGCTCCGGGTTGGGCTGAGATGGCTGTGCCCCA
ACTTCCCATTCACCCACCATGGACCCATAATTAACCTGGCCCCACCCCANTAAAAAAAAA
ANAAAAAACCCGACTCTAGATTGGCGCCCGCGTCATAGCTGTTTCCTGAAAAATCCCGGG
TGGCATCCCTTGAACCCCTCCCAGAGCCTTTCCTGCCCTGNAAGTTGCCTCCCGTGCCAC
CACCCTGNCCTATAAATTAGGTGCATCATTGTCTGACAGGGGCCTCCTAAATACGTGCGG
GGGCGCGGGGCCCGCCCTCTACCCCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_139208 unedited
NNAAAGATNTTTACTCTGNACCGCGGNCCCGCATTCNANGATCGAGTTTTTTTTTTTTTT
TTTTTTTGGGGTGGGGCCGGTTTATATTGGGTCCTGGGGGGTGAATGGGAGTTGGGGCAC
AGCCATCTCAGCCCAACCCGGAGCTGAGGCCAGCAGGCTGGCTCTGGCTTCTGACCTGCT
GGGCAGGCCGGAGCTCCAGGGGAGGCTAGAGGCTCTGCTCTGAGCAGGTGCGCTTGTTAC
GGTGCAAGACGTAGCCTGCGCGGCAGGAGCAGTAGAAACCGCCCAGGTGGTTGTGGCAGT
GGTGGTCGCAAGTGGGCGCCTCTCCCGGGGCCACCTGGCACTCGTCAATGTCCTCGGCTG
CATAGAAGGCCTCGAACCCCGTGAACGGCTTCTCGTTGGAGTAGTCGGAGCGGAAGGTAA
TGTCCAGGCTGGAGCCCAGCGAGTAGAAAGTGTCCTTGCCAGGGGCCCGCTCCGTGTCTG
TGCTCTCCTGCCCGCACAGCGTGGCCAGCACCTTGGCCCCCGAGCTCAGCTTGACGAAGT
CGTACTCGCAGAGGTGGGAGAGCTCCAGGTCGAAGTGGGTGAAGTAAAGGCGCAGGCGGT
AGCCGGGGGGTGCAGTCAGGGTCCAGCGCCGCTCCTGGTCATTGGCATACTCCCCTGGAA
AGCCGGGGGATGCCAGGCGCCCGACACAGGTTCAGGCCACTTCGGGCCCAAGGGGGGTGG
CCACCGAGCCACACAGAAGGCCCNAGNAGGTCAGCAGCCTCATGGTGTGCCCGTCCAGCT
GGCCTGGTGCCGANATCCGGCCGCCTATAGTGAGTCGTATACAAATTCTGACGGTCACTA
ACGAGCTCTGCTATATAGACTNCCACGTAACGCTACCGCCATTGCGTACGGGGCGGGGTA
TACGACATTG
Restriction Sites NotI-NotI |
ACCN NM_139208
Insert Size 780 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139208.1, NP_631947.1
RefSeq Size 738 bp
RefSeq ORF 558 bp
Locus ID 10747
UniProt ID O00187
Cytogenetics 1p36.22
Domains CUB, EGF, EGF_CA
Protein Families Druggable Genome, Protease, Secreted Protein
Protein Pathways Complement and coagulation cascades
Summary This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. provided by RefSeq, Feb 2016
Transcript Variant: This variant (2), also called MAp19, differs in the 3' UTR and 3' coding region compared to variant 1, resulting in a frameshift and upstream stop codon. Isoform 2 has a unique C-terminus and lacks the serine protease catalytic domain, compared to isoform 1.
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Documents
Resources
"NM_139208" in other vectors (4)
SKU Description Size Price
RC218844 MASP2 (Myc-DDK-tagged)-Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2 10 ug
$300.00
RC218844L3 Lenti ORF clone of Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC218844L4 Lenti ORF clone of Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2, mGFP tagged 10 ug
$600.00
RG218844 MASP2 (tGFP-tagged) - Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 2 10 ug
$489.00 $500.00

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