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Furin (NM_001081454) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210693
Furin (Myc-DDK-tagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $726.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Furin
Synonyms 9130404I01Rik; Fu; Fur; PA; PACE; Pcs; Pcsk3; SP; SPC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210693 representing NM_001081454
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGAGATCCTGGTTGCTATGGGTGGTCGCAGCAGCAGGAGCCGTGGTCCTGCTGGCAGCTGATG
CTCAAGGCCAGAAGATCTTCACCAACACCTGGGCCGTGCACATTCCTGGAGGCCCAGCTGTGGCTGATAG
GGTGGCGCAGAAGCATGGCTTCCACAACCTGGGCCAGATCTTCGGTGACTATTACCACTTCTGGCACAGA
GCAGTGACAAAGCGGTCCCTGTCGCCTCACCGCCCGCGGCACAGCCGGCTACAGAGGGAGCCTCAAGTAA
AGTGGCTGGAGCAGCAGGTAGCCAAGCGAAGAGCCAAGAGGGACGTGTATCAGGAGCCCACGGACCCCAA
GTTCCCCCAGCAGTGGTACCTGTCTGGTGTCACTCAGCGAGACCTGAATGTGAAGGAGGCCTGGGCCCAG
GGCTTCACAGGCCATGGCATTGTGGTCTCCATCCTGGATGACGGCATTGAGAAGAATCATCCCGACCTAG
CAGGCAATTATGACCCTGGAGCCAGTTTTGACGTGAATGACCAGGACCCCGACCCACAGCCTCGGTACAC
ACAGATGAATGACAACAGGCATGGCACTCGCTGTGCCGGGGAAGTGGCAGCAGTGGCCAACAATGGTGTC
TGTGGCGTAGGTGTAGCTTACAATGCCCGAATTGGAGGGGTGCGGATGTTGGATGGCGAGGTGACTGATG
CAGTAGAGGCACGTTCGCTGGGCCTGAATCCCAACCACATCCACATCTACAGCGCCAGCTGGGGCCCTGA
GGACGACGGCAAGACCGTGGATGGACCAGCCCGGCTCGCTGAGGAGGCCTTCTTTCGGGGAGTTAGCCAG
GGCCGAGGAGGGCTGGGCTCCATCTTTGTCTGGGCCTCAGGGAATGGGGGCCGGGAACATGACAGCTGCA
ACTGTGACGGCTACACCAACAGCATCTATACACTGTCCATCAGCAGCGCCACACAGTTCGGCAATGTGCC
CTGGTACAGTGAGGCCTGCTCCTCCACACTGGCCACCACCTACAGCAGCGGCAACCAGAATGAGAAGCAG
ATCGTGACAACTGACCTGAGGCAGAAGTGCACAGAATCTCACACAGGCACCTCGGCTTCTGCCCCCTTGG
CAGCTGGTATCATTGCTCTCACCCTGGAGGCCAACAAGAACCTCACCTGGCGGGACATGCAGCACCTGGT
AGTGCAGACCTCAAAGCCAGCCCACCTCAACGCTGATGATTGGGCTACCAACGGCGTGGGCCGGAAAGTG
AGCCATTCGTATGGCTACGGGCTGTTGGATGCAGGTGCCATGGTGGCTCTGGCCCAGAACTGGACAACAG
TGGCCCCCCAGCGGAAGTGCATTGTTGAAATCCTGGTGGAACCCAAGGACATCGGCAAACGGCTAGAGGT
GCGCAAGGCGGTGACAGCATGCCTGGGTGAGCCCAACCACATCACCAGGCTGGAACACGTTCAGGCCCGG
CTCACCCTGTCTTACAATCGCCGCGGTGACCTGGCTATCCACCTCATCAGCCCCATGGGCACCCGCTCCA
CCCTGTTAGCTGCCAGACCACATGACTACTCTGCTGATGGGTTTAATGATTGGGCTTTCATGACAACCCA
TTCCTGGGATGAGGATCCGGCTGGCGAATGGGTCCTAGAGATTGAAAATACCAGTGAAGCCAACAACTAT
GGGACGCTGACCAAGTTCACTCTCGTTCTGTATGGCACAGCCCCCGAGGGGCTCTCTACACCTCCAGAAA
GCAGCGGCTGCAAGACCCTCACATCCAGCCAGGCCTGCGTGGTGTGTGAGGAAGGTTACTCTCTGCACCA
GAAAAGCTGTGTCCAGCACTGCCCACCAGGCTTCATCCCCCAAGTCCTTGATACACACTACAGCACTGAG
AATGATGTGGAGATCATCCGTGCCAGCGTCTGTACCCCCTGCCACGCCTCATGTGCCACTTGCCAGGGCC
CGGCCCCCACAGACTGCCTCAGCTGCCCCAGCCATGCCTCCCTGGACCCAGTGGAGCAGACCTGCTCCCG
GCAAAGCCAGAGCAGCCGGGAGTCTCGGCCTCAGCAGCAGCCTCCTGCACTGCGCCCTGAGGTGGAGATG
GAGCCCCGGCTGCAGGCTGGGTTGGCCTCTCACCTGCCCGAGGTCCTGGCCGGCCTCAGCTGCCTTATCA
TCGTGCTCATCTTTGGCATCGTCTTCCTGTTCCTGCATCGGTGTTCGGGCTTCAGCTTCCGGGGAGTGAA
AGTGTACACCATGGACCGCGGCCTCATCTCCTACAAGGGGCTGCCCCCTGAAGCCTGGCAGGAGGAGTGC
CCATCGGACTCAGAGGAGGACGAGGGCCGGGGCGAGAGGACCGCCTTCATCAAAGACCAGAGCGCCCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210693 representing NM_001081454
Red=Cloning site Green=Tags(s)

MELRSWLLWVVAAAGAVVLLAADAQGQKIFTNTWAVHIPGGPAVADRVAQKHGFHNLGQIFGDYYHFWHR
AVTKRSLSPHRPRHSRLQREPQVKWLEQQVAKRRAKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKEAWAQ
GFTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGV
CGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQ
GRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQ
IVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNADDWATNGVGRKV
SHSYGYGLLDAGAMVALAQNWTTVAPQRKCIVEILVEPKDIGKRLEVRKAVTACLGEPNHITRLEHVQAR
LTLSYNRRGDLAIHLISPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPAGEWVLEIENTSEANNY
GTLTKFTLVLYGTAPEGLSTPPESSGCKTLTSSQACVVCEEGYSLHQKSCVQHCPPGFIPQVLDTHYSTE
NDVEIIRASVCTPCHASCATCQGPAPTDCLSCPSHASLDPVEQTCSRQSQSSRESRPQQQPPALRPEVEM
EPRLQAGLASHLPEVLAGLSCLIIVLIFGIVFLFLHRCSGFSFRGVKVYTMDRGLISYKGLPPEAWQEEC
PSDSEEDEGRGERTAFIKDQSAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081454
ORF Size 2379 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081454.2
RefSeq Size 4166 bp
RefSeq ORF 2382 bp
Locus ID 18550
UniProt ID P23188
Cytogenetics 7 45.65 cM
MW 87.2 kDa
Summary This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2015
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Documents
Resources
"NM_001081454" in other vectors (4)
SKU Description Size Price
MC202300 Furin (untagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 2, (10ug) 10 ug
$727.00
MG210693 Furin (tGFP-tagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin) 10 ug
$926.00
MR210693L3 Lenti ORF clone of Furin (Myc-DDK-tagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 2 10 ug
$1,026.00
MR210693L4 Lenti ORF clone of Furin (mGFP-tagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 2 10 ug
$1,026.00

Plasmid Map for MR210693

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210693
Circular map for MR210693

file-alt Citations

Gelatinases Cleave Dentin Sialoprotein Intracellularly
Gou, Xue, Zheng et al
Front Physiol (2020) 11, 686 DOI: 10.3389/fphys.2020.00686
Cited by 10
AI Summary: In this paper, OriGene products—including the MMP9 (Cat# RC218307) and FURIN (Cat# RC217963) expression plasmids—were used to overexpress these proteins in HEK293E cells, enabling the authors to investigate the intracellular cleavage and activation mechanisms involved in dentin sialoprotein processing.
FigureFigureFigure
Gelatinases Cleave Dentin Sialoprotein Intracellularly
Gou, Xue, Zheng et al
Front. Physiol. (2020) 11, 686 DOI: 10.3389/fphys.2020.00686
Cited by 10
AI Summary: OriGene contributed Furin (Myc-DDK-tagged, MR210693) and Mmp3 (Myc-DDK-tagged, MR207673) plasmids, which were used to study the intracellular processing and interactions of proteins involved in dentin sialoprotein cleavage.
Snippet: The plasmid pCMV3-mMmp9-Flag (MG50560-CF) was purchased from Sino Biological (Beijing, China). The plasmids of Furin (Myc-DDK-tagged) (MR210693) and Mmp3 (Myc-DDK-tagged) (MR207673) were purchased from ORIGENE (Beijing, China) Cached;- The plasmid pCMV3-mMmp9-Flag (MG50560-CF) was purchased from Sino Biological (Beijing, China). The plasmids of Furin (Myc-DDK-tagged) (MR210693) and Mmp3 (Myc-DDK-tagged) (MR207673) were purchased from ORIGENE (Beijing, China) Cached
Selective inhibition of N-linked glycosylation impairs receptor tyrosine kinase processing
Klaver, Zhao, May et al
Dis Model Mech (2019) 12 (6) DOI: 10.1242/dmm.039602
AI Summary: OriGene’s murine Flag-tagged Furin (MR210693) and Flag-tagged PCSK5 (MR222003) cDNA constructs were used to transiently transfect cells, enabling the study of how altered glycosylation affects the processing and function of these convertases in receptor tyrosine kinase maturation.
Snippet: … Cells were transiently transfected with murine Flag-tagged Furin (Origene, MR210693) or Flag-tagged PCSK5 (Origene, MR222003) using Lipofectamine 2000 Transfection Reagent (Thermo Fisher, 11668030). … One-step SEEL l;Cells were transiently transfected with murine Flag-tagged Furin (Origene, MR210693) or Flag-tagged PCSK5 (Origene, MR222003) using Lipofectamine 2000 Transfection Reagent (Thermo Fisher, 11668030)
N-linked glycosylation dependent and independent mechanisms regulating CTRP12 cleavage, secretion, and stability
Stewart, Tan, Clark et al
Biochemistry (2018)
AI Summary: OriGene provided untagged human GnTI/MGAT1 (NM_002406) and C-terminal FLAG-tagged mouse furin/PCSK3 (NM_001081454) expression plasmids, which were used to study the effects of glycosylation and protease activity on CTRP12 cleavage, secretion, and stability.
Snippet: ADVERTISEMENT;sequencing. Untagged human GnTI/MGAT1 (NM_002406) and C-terminal FLAG-tagged mouse furin/PCSK3 (NM_001081454) expression plasmids were obtained from Origene and verified by sequencing. Cell culture and transfection
Showing 1-4 of 4 papers.
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