SARS-CoV-2 S Gene cDNA Clone (Native Sequence)
- TrueORF®
SKU
VC202557
Native cDNA clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390
$589.00
$1,109.00
In Stock*
Specifications
| Product Data | |
| Type | Virus Untagged ORF Clone |
|---|---|
| Target Symbol | S Protein |
| Vector | pUCminusMCS |
| E. coli Selection | Ampicillin |
| Mammalian Cell Selection | None |
| ACCN | NC_045512 |
| ORF Size | 7314 bp |
| OTI Annotation | This clone was engineered to express the complete ORF. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NC_045512.2, YP_009724390 |
| RefSeq ORF | 7314 bp |
| Locus ID | 43740568 |
| MW | 141.2 kDa |
Reviews
Documents
| Product Manuals |
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Resources
"NC_045512" in other vectors (41)
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|---|---|---|---|---|
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| VC102556 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein extracellular domain [Severe acute respiratory syndrome coronavirus 2], YP_009724390 | 10 ug |
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| VC102557 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390. Note: ORF is codon optimized | 10 ug |
$589.00
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| VC102558 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391. Note: ORF is codon optimized | 10 ug |
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| VC102559 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724393. Note: ORF is codon optimized | 10 ug |
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| VC102560 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724394 | 10 ug |
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| VC102561 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724395 | 10 ug |
$150.00
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| VC102562 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724396 | 10 ug |
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| VC102563 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724397 | 10 ug |
$289.00
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| VC102564 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009725255 | 10 ug |
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| VC102565 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724392. Note: ORF is codon optimized | 10 ug |
$289.00
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| VC102566 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390 | 10 ug |
$589.00
$1,108.00
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| VC102567 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391 | 10 ug |
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| VC102568 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393 | 10 ug |
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| VC102569 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394 | 10 ug |
$150.00
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| VC102570 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395 | 10 ug |
$150.00
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|
| VC102571 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396 | 10 ug |
$150.00
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| VC102572 | Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255 | 10 ug |
$289.00
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| VC102573 | Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392 | 10 ug |
$289.00
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| VC102574 | mRFP-tagged ORF clone for SARS-CoV-2 NSP4 [Severe acute respiratory syndrome coronavirus 2], YP_009725300.1 | 10 ug |
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| VC102575 | mRFP-tagged ORF clone for SARS-CoV-2 NSP5 [Severe acute respiratory syndrome coronavirus 2], YP_009725301.1 | 10 ug |
$289.00
$300.00
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| VC102576 | mRFP-tagged ORF clone for SARS-CoV-2 NSP6 [Severe acute respiratory syndrome coronavirus 2], YP_009725302.1 | 10 ug |
$289.00
$300.00
|
|
| VC102577 | mRFP-tagged ORF clone for SARS-CoV-2 NSP7 [Severe acute respiratory syndrome coronavirus 2], YP_009725303.1 | 10 ug |
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|
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| VC102578 | mRFP-tagged ORF clone for SARS-CoV-2 NSP8 [Severe acute respiratory syndrome coronavirus 2], YP_009725304.1 | 10 ug |
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$300.00
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| VC102579 | mRFP-tagged ORF clone for SARS-CoV-2 NSP9 [Severe acute respiratory syndrome coronavirus 2], YP_009725305.1 | 10 ug |
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| VC102580 | mRFP-tagged ORF clone for SARS-CoV-2 NSP10 [Severe acute respiratory syndrome coronavirus 2], YP_009725306.1 | 10 ug |
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| VC102581 | mRFP-tagged ORF clone for SARS-CoV-2 NSP14 [Severe acute respiratory syndrome coronavirus 2], YP_009725309.1 | 10 ug |
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| VC102582 | mRFP-tagged ORF clone for SARS-CoV-2 ORF3a [Severe acute respiratory syndrome coronavirus 2], YP_009724391.1 | 10 ug |
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| VC102583 | mRFP-tagged ORF clone for SARS-CoV-2 M protein [Severe acute respiratory syndrome coronavirus 2], YP_009724393.1 | 10 ug |
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| VC102584 | mRFP-tagged ORF clone for SARS-CoV-2 ORF9b [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2 | 10 ug |
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| VC102585 | mRFP-tagged ORF clone for SARS-CoV-2 ORF9c [Severe acute respiratory syndrome coronavirus 2], YP_009724397.2 | 10 ug |
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| VC102586 | mRFP-tagged ORF clone for SARS-CoV-2 ORF10 [Severe acute respiratory syndrome coronavirus 2], YP_009725255.1 | 10 ug |
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| VC102587 | Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein mutant (D614G) [Severe acute respiratory syndrome coronavirus 2], YP_009724390 | 10 ug |
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| VC202558 | Native cDNA clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391 | 10 ug |
$300.00
|
|
| VC202559 | Native cDNA clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724393 | 10 ug |
$289.00
$300.00
|
|
| VC202560 | Native cDNA clone for SARS-CoV-2 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724394 | 10 ug |
$150.00
|
|
| VC202561 | Native cDNA clone for SARS-CoV-2 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724395 | 10 ug |
$150.00
|
|
| VC202562 | Native cDNA clone for SARS-CoV-2 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], YP_009724396 | 10 ug |
$150.00
|
|
| VC202563 | Native cDNA clone for SARS-CoV-2 Nucleocapsid Phosphoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724397 | 10 ug |
$289.00
$457.00
|
|
| VC202564 | Native cDNA clone for SARS-CoV-2 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], YP_009725255 | 10 ug |
$150.00
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|
| VC202565 | Native cDNA clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], YP_009724392 | 10 ug |
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|
Citations
AURKB, in concert with REST, acts as an oxygen-sensitive epigenetic regulator of the hypoxic induction of MDM2
Kim, Choi, Yoo et al
BMB Rep (2022) 55 (6), 287-292 DOI: 10.5483/BMBRep.2022.55.6.017
Kim, Choi, Yoo et al
BMB Rep (2022) 55 (6), 287-292 DOI: 10.5483/BMBRep.2022.55.6.017
AI Summary: In this study, the REST cDNA clone from OriGene Technologies was used to investigate the role of REST in the epigenetic regulation of MDM2 under hypoxic conditions.
Snippet: tion is described in Supplementary Materials and Methods. Plasmids and siRNAs AURKB cDNA was cloned by RT-PCR and inserted into pcDNA vector (Promega, Madison, WI). REST cDNA clone was obtained from OriGene Technologies (Rockville, MD). HA-scHIF-1α was constructed as previously described (34). Site-directed mutage-nesis kit was used to create the mutant constructs (Stratagene, La Jolla, CA). All
Engineered niches support the development of human dendritic cells in humanized mice
Anselmi, Vaivode, Dutertre et al
Nat Commun (2020) 11 (1), 2054 DOI: 10.1038/s41467-020-15937-y
Anselmi, Vaivode, Dutertre et al
Nat Commun (2020) 11 (1), 2054 DOI: 10.1038/s41467-020-15937-y
Cited by 31
AI Summary: In this paper, OriGene products—specifically the pCMV6-Entry vector—were used for the genetic engineering of mesenchymal stromal cells to express key hematopoietic factors, facilitating the development and study of human dendritic cells in humanized mice.
Vitamin E δ-tocotrienol sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis through proteasome-mediated down-regulation of c-FLIPs
Francois, Zhang, Husain et al
Cancer Cell Int (2019) 19, 189 DOI: 10.1186/s12935-019-0876-0
Francois, Zhang, Husain et al
Cancer Cell Int (2019) 19, 189 DOI: 10.1186/s12935-019-0876-0
Cited by 22
AI Summary: In this study, OriGene’s c-FLIP expression plasmid (RC200176) was used to enforce c-FLIP expression in pancreatic cancer cells, helping to demonstrate that c-FLIP reduction is critical for vitamin E δ-tocotrienol (VEDT) and TRAIL-induced apoptosis.
An unbiased in vivo functional genomics screening approach in mice identifies novel tumor cell-based regulators of immune rejection
Shuptrine, Ajina, Fertig et al
Cancer Immunol. Immunother. (2017)
Shuptrine, Ajina, Fertig et al
Cancer Immunol. Immunother. (2017)
AI Summary: OriGene’s CD47 cDNA plasmids were used to overexpress CD47 in EO771 cells, enabling the authors to validate the gene’s role in regulating tumor cell sensitivity to immune rejection in their functional genomics screening approach.
Snippet: Overexpression For CD47 overexpression, EO771 cells were transfected at 50% confluency in a 12-well dish with 1 μg cDNA plas- mids (Origene) and Fugene 6 (Promega;E2691) to induce expression of the desired transcript. ... All 2 versions Cite SaveSaving
STING agonists induce an innate antiviral immune response against hepatitis B virus
Guo, Han, Zhao et al
Antimicrob. Agents Chemother. (2014)
Guo, Han, Zhao et al
Antimicrob. Agents Chemother. (2014)
AI Summary: OriGene products contributed to this study by providing the pRS 143 vector used for constructing the shRNA targeting murine STING and a control plasmid expressing scrambled shRNA from the pRS vector.
Snippet: targeting murine STING was constructed by inserting the following cDNA sequence into pRS 143 vector (Origene): tcaatcagctacataacaactcgagttgttatgtagctgattga. A control plasmid expressing 144 scrambled shRNA from pRS vector was purchased from Origene. ... Cite SaveSaving
Amino acids activate mTOR complex1 without changing Rag GTPase guanyl nucleotide charging
Oshiro, Rapley, Avruch
J. Biol. Chem. (2013)
Oshiro, Rapley, Avruch
J. Biol. Chem. (2013)
AI Summary: OriGene contributed RagA, RagB, RagC, and RagD cDNA clones, which were used in this study to investigate the mechanisms of mTORC1 activation by amino acids.
Snippet: DNA constructs. RagA, B, C and D cDNA clones were purchased from Origene and PCR cloned into pEBG and pCMV5-FLAG expression vectors using Taq polymerase according to the manufacturers instructions (Roche). Mutations ... Cite SaveSaving
Showing 1-6 of 7 papers.
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