TMPRSS2 (NM_005656) Human Tagged ORF Clone
SKU
RC208677
TMPRSS2 (Myc-DDK-tagged)-Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TMPRSS2 |
| Synonyms | PRSS10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005656 |
| ORF Size | 1476 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005656.4 |
| RefSeq Size | 3212 bp |
| RefSeq ORF | 1479 bp |
| Locus ID | 7113 |
| UniProt ID | O15393 |
| Cytogenetics | 21q22.3 |
| Domains | ldl_recept_a, SR, Tryp_SPc |
| Protein Families | Druggable Genome, Protease, Secreted Protein, Transmembrane |
| MW | 53.9 kDa |
| Summary | This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005656" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208677L1 | Lenti ORF clone of Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC208677L2 | Lenti ORF clone of Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RC208677L3 | Lenti ORF clone of Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC208677L4 | Lenti ORF clone of Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RG208677 | TMPRSS2 (tGFP-tagged) - Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2 | 10 ug |
$489.00
$657.00
|
|
| SC116619 | TMPRSS2 (untagged)-Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2 | 10 ug |
$686.00
|
|
| SC323858 | TMPRSS2 (untagged)-Human transmembrane protease, serine 2 (TMPRSS2), transcript variant 2 | 10 ug |
$686.00
|
|
Citations
Host susceptibilities and entry processes of SARS-CoV-2 Omicron variants using pseudotyped viruses carrying spike protein
Zabiegala, Kim, Chang
BMC Vet Res (2025) 21 (1), 377 DOI: 10.1186/s12917-025-04822-9
Zabiegala, Kim, Chang
BMC Vet Res (2025) 21 (1), 377 DOI: 10.1186/s12917-025-04822-9
Cited by 1
AI Summary: OriGene products pCMV6-hDPP4 (#RC209466) and pCMV6-hTMPRSS2 (#RC208677) were used to express DPP4 and TMPRSS2 in HEK293T cells, enabling the study of how these proteins affect the entry of SARS-CoV-2 Omicron variant pseudoviruses.
Snippet: ned by transducing each in HEK293T cells expressing ACE2 or DPP4 with or without TMPRSS2. Briefly, one-day old HEK293T cells were transfected with pIRES-Neo-hACE2-FLAG [30] or pCMV6-hDPP4 (#RC209466, Origene, Rockville, MD) with or without pCMV6-hTMPRSS2 (#RC208677, Origene). The plasmid expressing Renilla luciferase, pRL-CMV (#E2231, Promega), was included as an expression control. The firefly lu
IFITM3 Inhibits SARS-CoV-2 Infection and Is Associated with COVID-19 Susceptibility
Xu, Wang, Zhao et al
Viruses (2022) 14 (11) DOI: 10.3390/v14112553
Xu, Wang, Zhao et al
Viruses (2022) 14 (11) DOI: 10.3390/v14112553
Cited by 40
AI Summary: OriGene’s TMPRSS2 expression plasmid (Cat. No. RC208677) was used in this study to facilitate experiments investigating the role of IFITM3 in inhibiting SARS-CoV-2 infection.
Snippet: based mutagenesis method as described previously [24,28]. The pCAGGS-SARS-CoV-2-S expression plasmid was a gift from He Huang (IPB, Beijing, China). The TMPRSS2 expression plasmid was purchased from Origene (Cat. No. RC208677). Transfection of plasmid DNA into cells was performed with Lipofectamine 2000 (Thermo Fisher Scientific, MA, USA) in accordance with the manufacturer’s instructions. 2.2
IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function
Casciola-Rosen, Thiemann, Andrade et al
JCI Insight (2022) 7 (9) DOI: 10.1172/jci.insight.158362
Casciola-Rosen, Thiemann, Andrade et al
JCI Insight (2022) 7 (9) DOI: 10.1172/jci.insight.158362
Cited by 91
AI Summary: In this study, OriGene’s full-length FLAG-tagged CD9 (RC202000) and TMPRSS2 (RC208677) cDNA constructs were used to transiently transfect HEK293T cells for immunoblot assays to detect antibodies against these proteins, aiding in the investigation of autoantibody responses in severe COVID-19.
Snippet: … Immunoblots of transfected lysates to detect antibodies against CD9, CD81, and TMPRSS2. Complementary DNAs encoding full-length FLAG-tagged CD9 and TMPRSS2 (OriGene RC202000 and RC208677, respectively) and CD81 (GenScript OHu19408) were sequence verified. These DNAs were transiently transfected into HEK293T cells (purchased from ATCC) with ExpiFectamin_29;Complementary DNAs encoding full-length FLAG-tagged CD9 and TMPRSS2 (OriGene RC202000 and RC208677, respectively) and CD81 (GenScript OHu19408) were sequence verified. …
Identification of Plitidepsin as Potent Inhibitor of SARS-CoV-2-Induced Cytopathic Effect After a Drug Repurposing Screen
Rodon, Muñoz-Basagoiti, Perez-Zsolt et al
Front Pharmacol (2021) 12, 646676 DOI: 10.3389/fphar.2021.646676
Rodon, Muñoz-Basagoiti, Perez-Zsolt et al
Front Pharmacol (2021) 12, 646676 DOI: 10.3389/fphar.2021.646676
Cited by 58
AI Summary: In this study, the TMPRSS2 human plasmid from OriGene was used to transfect HEK-293T cells, enabling the researchers to assess the impact of candidate antiviral drugs on SARS-CoV-2 entry and replication in cells engineered to express relevant human proteins.
Snippet: 100 μg/ml streptomycin, and 1 μg/ml of puromycin (all from Invitrogen). TMPRSS2 human plasmid (Origene) was transfected using X-tremeGENE HP Transfection Reagent (Merck) on HEK- 293T overexpressing the human ACE2
Deep mutational scanning of hemagglutinin helps predict evolutionary fates of human H3N2 influenza variants
Lee, Huddleston, Doud et al
Proc Natl Acad Sci U S A (2018) 115 (35), E8276-E8285 DOI: 10.1073/pnas.1806133115
Lee, Huddleston, Doud et al
Proc Natl Acad Sci U S A (2018) 115 (35), E8276-E8285 DOI: 10.1073/pnas.1806133115
Cited by 245
AI Summary: In this study, OriGene's pCMV6-Entry-HA plasmid was used to express hemagglutinin (HA) for deep mutational scanning experiments, enabling the systematic analysis of how individual HA mutations affect influenza virus growth and evolutionary potential.
Showing 1-5 of 5 papers.
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