FURIN (NM_002569) Human 3' UTR Clone

SKU SC215432

3' UTR clone of furin (paired basic amino acid cleaving enzyme) (FURIN) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: FURIN
• Synonyms: FUR; PACE; PCSK3; SPC1
• ACCN: NM_002569
• Insert Size: 1609 bp
• Sequence Data:
  Insert Sequence

  >SC215432 3’UTR clone of NM_002569 ; The sequence shown below is from the reference sequence of NM_002569. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GCCTTTATCAAAGACCAGAGCGCCCTCTGATGAGCCCACTGCCCACCCCCTCAAGCCAATCCCCTCCTT; GGGCACTTTTTAATTCACCAAAGTATTTTTTTATCTTGGGACTGGGTTTGGACCCCAGCTGGGAGGCAA; GAGGGGTGGAGACTGCTTCCCATCCTACCCTCGGGCCCACCTGGCCACCTGAGGTGGGCCCAGGACCAG; CTGGGGCGTGGGGAGGGCCGTACCCCACCCTCAGCACCCCTTCCATGTGGAGAAAGGAGTGAAACCTTT; AGGGCAGCTTGCCCCGGCCCCGGCCCCAGCCAGAGTTCCTGCGGAGTGAAGAGGGGCAGCCCTTGCTTG; TTGGGATTCCTGACCCAGGCCGCAGCTCTTGCCCTTCCCTGTCCCTCTAAAGCAATAATGGTCCCATCC; AGGCAGTCGGGGGCTGGCCTAGGAGATATCTGAGGGAGGAGGCCACCTCTCCAAGGGCTTCTGCACCCT; CCACCCTGTCCCCCAGCTCTGGTGAGTCTTGGCGGCAGCAGCCATCATAGGAAGGGACCAAGGCAAGGC; AGGTGCCTCCAGGTGTGCACGTGGCATGTGGCCTGTGGCCTGTGTCCCATGACCCACCCCTGTGCTCCG; TGCCTCCACCACCACTGGCCACCAGGCTGGCGCAGCCAAGGCCGAAGCTCTGGCTGAACCCTGTGCTGG; TGTCCTGACCACCCTCCCCTCTCTTGCACCCGCCTCTCCCGTCAGGGCCCAAGTCCCTGTTTTCTGAGC; CCGGGCTGCCTGGGCTGTTGGCACTCACAGACCTGGAGCCCCTGGGTGGGTGGTGGGGAGGGGCGCTGG; CCCAGCCGGCCTCTCTGGCCTCCCACCCGATGCTGCTTTCCCCTGTGGGGATCTCAGGGGCTGTTTGAG; GATATATTTTCACTTTGTGATTATTTCACTTTAGATGCTGATGATTTGTTTTTGTATTTTTAATGGGGG; TAGCAGCTGGACTACCCACGTTCTCACACCCACCGTCCGCCCTGCTCCTCCCTGGCTGCCCTGGCCCTG; AGGTGTGGGGGCTGCAGCATGTTGCTGAGGAGTGAGGAATAGTTGAGCCCCAAGTCCTGAAGAGGCGGG; CCAGCCAGGCGGGCTCAAGGAAAGGGGGTCCCAGTGGGAGGGGCAGGCTGACATCTGTGTTTCAAGTGG; GGCTCGCCATGCCGGGGGTTCATAGGTCACTGGCTCTCCAAGTGCCAGAGGTGGGCAGGTGGTGGCACT; GAGCCCCCCCAACACTGTGCCCTGGTGGAGAAAGCACTGACCTGTCATGCCCCCCTCAAACCTCCTCTT; CTGACGTGCCTTTTGCACCCCTCCCATTAGGACAATCAGTCCCCTCCCATCTGGGAGTCCCCTTTTCTT; TTCTACCCTAGCCATTCCTGGTACCCAGCCATCTGCCCAGGGGTGCCCCCTCCTCTCCCATCCCCCTGC; CCTCGTGGCCAGCCCGGCTGGTTTTGTAAGATGCTGGGTTGGTGCACAGTGATTTTTTTCTTGTAATTT; AAACAGGCCCAGCATTGCTGGTTCTATTTAATGGACATGAGATAATGTTAGAGGTTTTAAAGTGATTAA; ACGTGCAGACTATGCAAACCAG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002569.4
• Locus ID: 5045
• Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]