Tmprss2 (NM_015775) Mouse Tagged ORF Clone

SKU MG207852

Tmprss2 (tGFP-tagged) - Mouse transmembrane protease, serine 2 (Tmprss2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Tmprss2
• Synonyms: D16Ertd61e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207852 representing NM_015775; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAACCACGGGTATCAGTCTGAGC; ACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTATCCAGCCCAGTACTACCCATC; TCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTGTCATCCACACACATCCCAAG; TCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGCCCTCGCCCTGGGCACTGTCC; TCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGCAACTGTTCTACGTCTGAGAT; GGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACGGGGTAGCACATTGTCCCAAC; GGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCTCCAGGTTTACTCATCTCAGA; GGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGGAGAGCAGCATGTAAAGACAT; GGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAG; CTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAGTGACTCATGTTCATCCCGCA; TGGTGGTTTCTTTGCGCTGTATAGAATGCGGGGTTCGCTCAGTGAAACGCCAGAGCAGGATTGTGGGTGG; ATTGAATGCCTCACCAGGAGACTGGCCCTGGCAGGTCAGCCTGCACGTCCAAGGCGTCCACGTCTGCGGA; GGCTCCATCATCACCCCCGAGTGGATTGTGACGGCCGCCCACTGTGTGGAAGAACCCCTCAGCAGCCCGA; GGTACTGGACGGCATTTGCGGGAATTCTGAGACAGTCTCTCATGTTCTATGGAAGTAGACACCAGGTAGA; AAAAGTAATTTCCCATCCAAATTACGACTCTAAGACCAAGAATAACGACATTGCTCTCATGAAGCTGCAG; ACACCTTTGGCTTTTAATGATCTAGTGAAGCCAGTGTGTCTGCCGAACCCAGGCATGATGCTAGACCTAG; ACCAGGAATGCTGGATTTCGGGGTGGGGGGCCACCTATGAGAAAGGGAAGACCTCGGACGTGTTGAATGC; TGCCATGGTACCCTTGATCGAGCCCTCCAAATGTAATAGTAAATACATATACAACAACCTAATCACACCA; GCCATGATCTGTGCCGGCTTCCTCCAGGGGTCTGTCGACTCTTGCCAGGGAGACAGTGGAGGGCCGCTGG; TTACTTTGAAGAATGGGATCTGGTGGCTGATTGGGGACACGAGCTGGGGCTCGGGCTGTGCCAAGGCACT; CAGACCTGGAGTATACGGGAACGTGACGGTATTTACAGATTGGATCTACCAGCAAATGAGGGCGAACAGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207852 representing NM_015775; Red=Cloning site Green=Tags(s); ; MALNSGSPPGIGPCYENHGYQSEHICPPRPPVAPNGYNLYPAQYYPSPVPQYAPRITTQASTSVIHTHPK; SSGALCTSKSKKSLCLALALGTVLTGAAVAAVLLWRFWDSNCSTSEMECGSSGTCISSSLWCDGVAHCPN; GEDENRCVRLYGQSFILQVYSSQRKAWYPVCQDDWSESYGRAACKDMGYKNNFYSSQGIPDQSGATSFMK; LNVSSGNVDLYKKLYHSDSCSSRMVVSLRCIECGVRSVKRQSRIVGGLNASPGDWPWQVSLHVQGVHVCG; GSIITPEWIVTAAHCVEEPLSSPRYWTAFAGILRQSLMFYGSRHQVEKVISHPNYDSKTKNNDIALMKLQ; TPLAFNDLVKPVCLPNPGMMLDLDQECWISGWGATYEKGKTSDVLNAAMVPLIEPSKCNSKYIYNNLITP; AMICAGFLQGSVDSCQGDSGGPLVTLKNGIWWLIGDTSWGSGCAKALRPGVYGNVTVFTDWIYQQMRANS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015775
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015775.2, NP_056590.2
• RefSeq Size: 3175 bp
• RefSeq ORF: 1473 bp
• Locus ID: 50528
• UniProt ID: Q9JIQ8
• Cytogenetics: 16 57.53 cM
• Summary: Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. The spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.[UniProtKB/Swiss-Prot Function]