Dendritic Cells

SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.

Flow cytometric analysis of living 293T cells transfected with BTLA overexpression plasmid (RC219458), Red)/empty vector (PS100001, Blue) using anti-BTLA antibody (TA812577). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Circular map for MR219259

Circular map for RG216428

Siva1 Mouse Gene Knockout Kit (CRISPR)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human CD163 plasmid (RC208557, lane 2), mouse CD163 plasmid (MR216798, lane 3) , rat CD163 plasmid (RR206586, lane 4) using anti-CD163 antibody TA506380 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Circular map for RC220974L4

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800091) (1:1200)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human DPP4 plasmid (RC209466, lane 2), mouse DPP4 plasmid (MR222698, lane 3) using anti-DPP4 antibody TA500733 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human IFIT1 plasmid (RC203320, lane 2), mouse IFIT1 plasmid (MR207393, lane 3) using anti-IFIT1 antibody TA500948 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human normal breast tissue and breast adenocarcinoma tissue using anti-MUC1 mouse monoclonal antibody. (UM800008; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:100)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTN3A2 (Cat# RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2(Cat# TA500730). Positive lysates LY402082 (100ug) and LC402082 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Human LAMP3 activation kit by CRISPRa

Coomassie blue staining of purified IFNA21 protein (Cat# TP310115). The protein was produced from HEK293T cells transfected with IFNA21 cDNA clone (Cat# RC210115) using MegaTran 2.0 (Cat# TT210002).

Western blot validation of overexpression lysate (Cat# LY403535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210450 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800091) (1:1200)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800090) (1:1200)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human IFIT1 plasmid (RC203320, lane 2), mouse IFIT1 plasmid (MR207393, lane 3) using anti-IFIT1 antibody TA500948 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BSG (Cat# RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG (Cat# TA501189). Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD80 (Cat# RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80(Cat# TA501576).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MRC2 (Cat# RC221360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRC2(Cat# TA811858). Positive lysates LY416912 (100ug) and LC416912 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800089) (1:200)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD274 (Cat# RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274(Cat# TA507087). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTLA (Cat# RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA(Cat# TA505688). Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDGFRB mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD274 (Cat# RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274(Cat# TA507087). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MUC1 (Cat# RC221390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MUC1(Cat# TA800790). Positive lysates LY422694 (100ug) and LC422694 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800139) (1:1200)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800090) (1:1200)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FGFR2 (Cat# RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2(Cat# TA502799). Positive lysates LY400050 (100ug) and LC400050 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) human tonsil tissue using anti-PD-L1 antibodies from 4 companies. (Heat-induced epitope retrieval by Retrieval Solution (20x)) – Data from Dr. Willa Shi, University Health Network.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-EPCAM mouse monoclonal antibody. (UM500097; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)