Integrin beta 1 (ITGB1) (NM_002211) Human Tagged ORF Clone
SKU
RC203818
ITGB1 (Myc-DDK-tagged)-Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A
$731.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Integrin beta 1 |
| Synonyms | CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002211 |
| ORF Size | 2394 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002211.4 |
| RefSeq Size | 3879 bp |
| RefSeq ORF | 2397 bp |
| Locus ID | 3688 |
| UniProt ID | P05556 |
| Cytogenetics | 10p11.22 |
| Domains | INB, PSI |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Axon guidance, Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer |
| MW | 88.4 kDa |
| Summary | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002211" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203818L1 | Lenti ORF clone of Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A, Myc-DDK-tagged | 10 ug |
$1,031.00
|
|
| RC203818L2 | Lenti ORF clone of Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A, mGFP tagged | 10 ug |
$1,031.00
|
|
| RC203818L3 | Lenti ORF clone of Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A, Myc-DDK-tagged | 10 ug |
$1,031.00
|
|
| RC203818L4 | Lenti ORF clone of Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A, mGFP tagged | 10 ug |
$1,031.00
|
|
| RG203818 | ITGB1 (tGFP-tagged) - Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A | 10 ug |
$789.00
$931.00
|
|
| SC111935 | ITGB1 (untagged)-Human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A | 10 ug |
$1,097.00
|
|
Citations
Biological Characteristics and Clinical Significance of ITGB1 and RHOC in Patients With Recurrent Colorectal Cancer
Ha, Tak, Kim et al
Anticancer Res. (2019) 39 (9), 4853-4864 DOI: 10.21873/anticanres.13671
Ha, Tak, Kim et al
Anticancer Res. (2019) 39 (9), 4853-4864 DOI: 10.21873/anticanres.13671
Cancer-associated fibroblast (CAF)-derived IL32 promotes breast cancer cell invasion and metastasis via integrin β3-p38 MAPK signalling
Wen, Hou, Fu et al
Cancer Lett. (2019) 442, 320-332 DOI: 10.1016/j.canlet.2018.10.015
Wen, Hou, Fu et al
Cancer Lett. (2019) 442, 320-332 DOI: 10.1016/j.canlet.2018.10.015
AI Summary: OriGene contributed integrin β3-, integrin β4-, and integrin β1-encoding vectors used in this study to investigate the role of integrin β3 in CAF-derived IL32-mediated breast cancer cell invasion and metastasis.
Snippet: The human integrin β3-, integrin β4-, integrin β1-encoding vectors were obtained from Addgene (www.addgene.org) or Origene (www.origene.com.cn). For all the short hairpin RNA (shRNA) oligonucleotides, synthetic DNA inserts were cloned into the pGLVH1/GFP vector (GenePharma, China). The target sequences of integrin β3 shRNAs were shRNA-1: 5'-GAATTGTACCTATAAGAAT-3', and shRNA-2: 5'-GAAAATCCGTTCTAAAG...
TGF-β triggers rapid fibrillogenesis via a novel TβRII dependent fibronectin trafficking mechanism
Varadaraj, Jenkins, Singh et al
Mol. Biol. Cell (2017)
Varadaraj, Jenkins, Singh et al
Mol. Biol. Cell (2017)
AI Summary: OriGene contributed commercially obtained human receptor clones used in this study to investigate the role of TGF-β and TβRII in fibronectin trafficking and fibrillogenesis.
Snippet: Page 17. 17 commercially obtained clones of the human receptors (OriGene);the coding regions were inserted N-terminally to mRFP (Ehrlich et al., 2004) or EGFP (Clontech) by PCR. TßRII knockdown: Two different Mission shRNA sequences from Sigma-Aldrich were ... All 2 versions Cite SaveSaving
PRL-3/PTP4A3 phosphatase regulates integrin β1 in adhesion structures during migration of human ocular melanoma cells
Foy, Anézo, Saule et al
Exp. Cell Res. (2017)
Foy, Anézo, Saule et al
Exp. Cell Res. (2017)
AI Summary: In this study, the pCMV6-Entry ITGB1A-FLAG plasmid from OriGene was used to investigate the role of integrin β1 in PRL-3-induced migration and adhesion of human ocular melanoma cells.
Snippet: IMCB Institute, Singapore). The plasmid pcDNA3.1-EGFPc1 was purchased from Clontech, psi-mU6 shRNA anti-ITGB1-CMV mCherryFP from GeneCopoeia, and pCMV6-Entry ITGB1A-FLAG from Origene. Mouse monoclonal ... All 2 versions Cite SaveSaving
Patient-Specific iPSC-Derived Endothelial Cells Uncover Pathways that Protect against Pulmonary Hypertension in BMPR2 Mutation Carriers
Gu, Shao, Sa et al
Cell Stem Cell (2016)
Gu, Shao, Sa et al
Cell Stem Cell (2016)
AI Summary: OriGene products, specifically the ITGB1 (Myc-DDK-tagged) plasmid (RC203818) and LRP1 (Myc-DDK-tagged) plasmid (RC218369), were used in this study to investigate the roles of integrin beta 1 and LRP1 in endothelial cell function and signaling pathways related to pulmonary hypertension.
Constitutive Association of Tie1 and Tie2 with Endothelial Integrins is Functionally Modulated by Angiopoietin-1 and Fibronectin
Dalton, Shlamkovitch, Papo et al
PLoS ONE (2016) 11 (10), e0163732 DOI: 10.1371/journal.pone.0163732
Dalton, Shlamkovitch, Papo et al
PLoS ONE (2016) 11 (10), e0163732 DOI: 10.1371/journal.pone.0163732
AI Summary: OriGene’s TrueORF gold cDNA constructs for human β1 (RC203818) and human β3 (RC201151) integrins were used to generate expression constructs essential for studying Tie-integrin interactions in endothelial cells.
Snippet: … Human ß1 (residues 1–728) (TrueORF gold cDNA construct RC203818 (Origene Technologies, inc.)) and human ß3 (residues 1–718) (TrueORF gold cDNA construct RC201151 (Origene Technologies, inc.)) were amplified and integrated into a pCDNA3.1(+)-Fc neo containing backbone by overlap PCR introducing a thrombin cleavage site at the C-terminus of the protein and before an Fc fragment
Showing 1-6 of 9 papers.
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