PD-L1 (CD274) Mouse Monoclonal Antibody [Clone ID: OTI9E12]

SKU CF808771

Carrier-free (BSA/glycerol-free) CD274 mouse monoclonal antibody, clone OTI9E12 (formerly 9E12)

Product Data

• Clone Name: OTI9E12
• Application: ELISA, FC, IF, LMNX, WB
• Recommended Dilution: WB 1:500
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-239 of human PD-L1/CD274(NP_054862) produced in HEK293 cells.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 31 kDa
• Gene Name: CD274 molecule
• Database Link:
  NP_054862
  Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)