WNT Pathway

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1. Positive lysates LY403455 (100ug) and LC403455 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PTK7 plasmid (RC223416, hPTK7), mouse PTK7 plasmid (MR211564, mPTK7) using anti-PTK7 antibody TA503920 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANAPC2 (Cat# RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2(Cat# TA503411). Positive lysates LY402247 (100ug) and LC402247 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PTK7 plasmid (RC223416, hPTK7), mouse PTK7 plasmid (MR211564, mPTK7) using anti-PTK7 antibody TA503920 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WNT3 (Cat# RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3(Cat# TA801867). Positive lysates LY403082 (100ug) and LC403082 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein ROR2 from HELA lysates (0.5 mg/mL) using ROR2 Mouse Monoclonal Antibody #TA810020. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Serum concentrations (ng/ml) of anti-ROR1 autoantibodies in chronic lymphocytic leukemia (CLL) patients and controls, as measured by ELISA against the full-length ROR1 protein (OriGene TP314967). Solid circles: non-progressive CLL; crosses: progressive CLL; blank circles: control donors. Dotted line represents mean+2SD of controls. *** p < 0.0001. Figure cited from PLoS ONE, PMID: 26562161

Circular map for RC218918

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY JNK1 (Cat# RC218407, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1(Cat# TA500081). Positive lysates LY400970 (100ug) and LC400970 (20ug) can be purchased separately from OriGene.

Circular map for RC224345

Circular map for RC208663

Circular map for RC218407

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CSNK1E (Cat# RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1E(Cat# TA803799). Positive lysates LY403455 (100ug) and LC403455 (20ug) can be purchased separately from OriGene.

Circular map for RC202926

Circular map for RC214967

Circular map for RC215640

Circular map for RC209206

Coomassie blue staining of purified WNT5A protein (Cat# TP309206). The protein was produced from HEK293T cells transfected with WNT5A cDNA clone (Cat# RC209206) using MegaTran 2.0 (Cat# TT210002).

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ROR1 plasmid (RC214967, lane 2), mouse ROR1 plasmid (MR224803, lane 3), rat ROR1 plasmid (RR214709, lane 4), using anti-ROR1 antibody TA806826 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Circular map for RC204636

Circular map for RC213639

Coomassie blue staining of purified LRP6 protein (Cat# TP318918). The protein was produced from HEK293T cells transfected with LRP6 cDNA clone (Cat# RC218918) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified PTK7 protein (Cat# TP323416). The protein was produced from HEK293T cells transfected with PTK7 cDNA clone (Cat# RC223416) using MegaTran 2.0 (Cat# TT210002).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY JNK1 (Cat# RC218407, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1(Cat# TA500081). Positive lysates LY400970 (100ug) and LC400970 (20ug) can be purchased separately from OriGene.

Coomassie blue staining of purified LRP5 protein (Cat# TP318864). The protein was produced from HEK293T cells transfected with LRP5 cDNA clone (Cat# RC218864) using MegaTran 2.0 (Cat# TT210002).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK9 (Cat# RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9 (Cat# TA505911). Positive lysates LY408411 (100ug) and LC408411 (20ug) can be purchased separately from OriGene.

Circular map for RC217286

Circular map for RC203451

Circular map for RC206570

Circular map for RC217764

Circular map for RC225565

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANAPC2 (Cat# RC207539, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2(Cat# TA503411). Positive lysates LY402247 (100ug) and LC402247 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANAPC11 (Cat# RC200097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC11(Cat# TA506325). Positive lysates LY424181 (100ug) and LC424181 (20ug) can be purchased separately from OriGene.

Coomassie blue staining of purified ANAPC2 protein (Cat# TP307539). The protein was produced from HEK293T cells transfected with ANAPC2 cDNA clone (Cat# RC207539) using MegaTran 2.0 (Cat# TT210002).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LEF1 (Cat# RC208663, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEF1 (Cat# TA812988)(1:500). Positive lysates LY402529 (100ug) and LC402529 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7.

Circular map for RC215449

Circular map for RC202436

Circular map for RC217936

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3. Positive lysates LY403082 (100ug) and LC403082 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROR2 (RC215640, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROR2 (1:2000). Positive lysates LY417906 (100ug) and LC417906 (20ug) can be purchased separately from OriGene.

Coomassie blue staining of purified RORA protein (Cat# TP302926). The protein was produced from HEK293T cells transfected with RORA cDNA clone (Cat# RC202926) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC207328

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CSNK1G1 (Cat# RC224794, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1G1(Cat# TA806335). Positive lysates LY411816 (100ug) and LC411816 (20ug) can be purchased separately from OriGene.

Circular map for RC210245

Circular map for RC210394

Circular map for RC210402

Circular map for RC222925

Coomassie blue staining of purified TCF4 protein (Cat# TP324345). The protein was produced from HEK293T cells transfected with TCF4 cDNA clone (Cat# RC224345) using MegaTran 2.0 (Cat# TT210002).