CCN5 (NM_003881) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CCN5 |
| Synonyms | CT58; CTGF-L; WISP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003881 |
| ORF Size | 750 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003881.3 |
| RefSeq Size | 1433 bp |
| RefSeq ORF | 753 bp |
| Locus ID | 8839 |
| UniProt ID | O76076 |
| Cytogenetics | 20q13.12 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| MW | 26.8 kDa |
| Summary | This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003881" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204636L1 | Lenti ORF clone of Human WNT1 inducible signaling pathway protein 2 (WISP2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204636L2 | Lenti ORF clone of Human WNT1 inducible signaling pathway protein 2 (WISP2), mGFP tagged | 10 ug |
$750.00
|
|
| RC204636L3 | Lenti ORF clone of Human WNT1 inducible signaling pathway protein 2 (WISP2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204636L4 | Lenti ORF clone of Human WNT1 inducible signaling pathway protein 2 (WISP2), mGFP tagged | 10 ug |
$750.00
|
|
| RG204636 | WISP2 (tGFP-tagged) - Human WNT1 inducible signaling pathway protein 2 (WISP2) | 10 ug |
$650.00
|
|
| SC122664 | WISP2 (untagged)-Human WNT1 inducible signaling pathway protein 2 (WISP2) | 10 ug |
$450.00
|
|
Citations
Expression and biological function of the cellular communication network factor 5 (CCN5) in primary liver cells
Borkham-Kamphorst, Meurer, Weiskirchen
J Cell Commun Signal (2023)
Borkham-Kamphorst, Meurer, Weiskirchen
J Cell Commun Signal (2023)
Cited by 5
Deficiency of CCN5/WISP-2-Driven Program in breast cancer Promotes Cancer Epithelial cells to mesenchymal stem cells and Breast Cancer growth
Das, Dhar, Maity et al
Sci Rep (2017) 7 (1), 1220 DOI: 10.1038/s41598-017-00916-z
Das, Dhar, Maity et al
Sci Rep (2017) 7 (1), 1220 DOI: 10.1038/s41598-017-00916-z
AI Summary: OriGene's CCN5 expression vectors were used to overexpress CCN5 in breast cancer cell populations, enabling the study of CCN5's role in reversing EMT signaling and inhibiting tumor growth.
Snippet: Indianapolis, IN, USA). Transfection, Recombinant Protein Treatment and Western Blotting. Expression vectors containing CCN5 or expression vectors alone were obtained from OriGene Technologies, Inc. (Rockville, MD, USA ... All 3 versions Cite SaveSaving
Showing 1-2 of 2 papers.
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