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RYK (NM_001005861) Human Tagged ORF Clone

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SKU
RC215449
RYK (Myc-DDK-tagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1
ORF Plasmid:
DDK DDK-His tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK DDK
  $568.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RYK
Synonyms D3S3195; JTK5; JTK5A; RYK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215449 representing NM_001005861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC
CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC
CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG
GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT
ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC
CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT
TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG
ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA
TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT
AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAG
GGGTTTGTTGTGCAGTAATATTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAG
GATTGAACTGGATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG
ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGCTCCTTAGGTTATCCTACCT
TGCGGATAGAGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAAGGCAAGGTGAAGGATATAGC
AATATCCAGAGAGAGGATAACTCTAAAAGATGTACTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGG
ATTTTAATAGATGAAAAAGATCCAAATAAAGAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTT
CTGAAATTCAGGTGACAATGATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCT
TCCTATTACTCATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGG
AATCTTAAATTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATTTCTCAGCAAG
ACCTGGTACACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCCAGAAGGGAAGTCATCCACAA
AGACCTGGCTGCCAGGAACTGTGTCATTGATGACACACTTCAAGTTAAGATCACAGACAATGCCCTCTCC
AGAGACTTGTTCCCCATGGACTATCACTGTCTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTC
TTGAAAGTCTGGTTAATAACGAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGA
ACTCATGACTCTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGAT
GGTTACCGAATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGTTGCTGGGCCT
TAGATCCAGAGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACAGAGTTTCATGCAGCCCTGGG
GGCCTACGTC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215449 representing NM_001005861
Red=Cloning site Green=Tags(s)

MRGAARLGRPGRSCLPGARGLRAPPPPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSE
DEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNI
SVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNT
SRTIYDPVHAAPTTSTRVFYISVGVCCAVIFLVAIILAVLHLHSMKRIELDDSISASSSSQGLSQPSTQT
TQYLRADTPNNATPITSSLGYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHG
ILIDEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWG
NLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALS
RDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKD
GYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005861
ORF Size 1830 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005861.2, NP_001005861.1
RefSeq Size 2960 bp
RefSeq ORF 1833 bp
Locus ID 6259
UniProt ID P34925, Q8WTZ8, Q59FQ5
Cytogenetics 3q22.2
Protein Families Druggable Genome, Protein Kinase, Transmembrane
MW 67.9 kDa
Summary The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Feb 2012
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"NM_001005861" in other vectors (7)
SKU Description Size Price
RC215449L1 Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, Myc-DDK-tagged 10 ug
$868.00
RC215449L2 Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, mGFP tagged 10 ug
$868.00
RC215449L3 Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, Myc-DDK-tagged 10 ug
$868.00
RC215449L4 Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, mGFP tagged 10 ug
$868.00
RC600067 RYK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens receptor-like tyrosine kinase, transcript variant 1, Signal peptide (1-25) plus EC domain (26-224) 10 ug
$289.00 $300.00
RG215449 RYK (tGFP-tagged) - Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1 10 ug
$768.00
SC301044 RYK (untagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1 10 ug
$569.00

Plasmid Map for RC215449

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215449
Circular map for RC215449

file-alt Citations

The Wnt Receptor Ryk Reduces Neuronal and Cell Survival Capacity by Repressing FOXO Activity During the Early Phases of Mutant Huntingtin Pathogenicity
Tourette, Farina, Vazquez-Manrique et al
PLoS Biol. (2014) 12 (6), e1001895 DOI: 10.1371/journal.pbio.1001895
Cited by 44
AI Summary: In this study, the OriGene product pCMV6-Myc-Ryk plasmid was used as the source for generating Myc-Ryk expression constructs, enabling the investigation of Ryk's role in neuronal survival and FOXO activity in Huntington's disease models.
Snippet: Normal htt mouse striatal cells derived from HdhQ111 mice and used at low passage numbers (P9–P12) were seeded in 96-well plates, at a density of 25,000 cells/well, and were co-transfected using Amaxa technology with 0.5 µg of the plasmid(s) pcDNA3.1-Foxo3a-HA (a gift from Lisa Ellerby) together with pcDNA3.1-Myc-Ryk (see below), pcDNA3.1-Myc-Ryk ICD (see above), pcDNA3.1-Myc-uncleavable-Ryk (see above), 100 nM of β-catenin siRNAs (QIAGEN) or scramble RNAs (Operon), 0.5 µg of the luciferase reporter (FHRE-luc, Addgene) [55], which contains three canonical FOXO binding sites, and 50 ng of the Renilla luciferase construct (Promega). The pcDNA3.1-Myc-Ryk construct was generated as follows: the Myc-Ryk coding sequence was amplified from pCMV6-Myc-Ryk (Origene) by using the primers forward 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCGCCAC​CATGCGCGCGGGCCGGGGC and reverse 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTATCAGACG​TAGGCTCCCAGGGCAG and subcloned into pcDNA3.1, and the resulting plasmid was verified for sequence integrity. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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