LEF1 (NM_016269) Human Tagged ORF Clone
SKU
RC208663
LEF1 (Myc-DDK-tagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LEF1 |
| Synonyms | LEF-1; TCF1ALPHA; TCF7L3; TCF10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016269 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016269.5 |
| RefSeq Size | 3620 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 51176 |
| UniProt ID | Q9UJU2 |
| Cytogenetics | 4q25 |
| Domains | HMG |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway |
| MW | 44.2 kDa |
| Summary | This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016269" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208663L1 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC208663L2 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC208663L3 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC208663L4 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG208663 | LEF1 (tGFP-tagged) - Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1 | 10 ug |
$489.00
$886.00
|
|
| SC114370 | LEF1 (untagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1 | 10 ug |
$686.00
|
|
Citations
Colorectal Cancer-Associated Smad4 R361 Hotspot Mutations Boost Wnt/β-Catenin Signaling through Enhanced Smad4-LEF1 Binding
Lanauze, Sehgal, Hayer et al
Mol Cancer Res (2021) 19 (5), 823-833 DOI: 10.1158/1541-7786.MCR-20-0721
Lanauze, Sehgal, Hayer et al
Mol Cancer Res (2021) 19 (5), 823-833 DOI: 10.1158/1541-7786.MCR-20-0721
AI Summary: In this study, OriGene products—specifically SMAD4 Myc-DDK-tagged expression plasmids—were used to re-express wild-type and mutant Smad4 proteins in colorectal cancer cell lines, enabling the authors to investigate how R361 hotspot mutations affect Wnt/β-catenin signaling.
Transplantation of hMSCs Genome Edited with LEF1 Improves Cardio-Protective Effects in Myocardial Infarction
Cho, Lee, Shen et al
Mol Ther Nucleic Acids (2020) 19, 1186-1197 DOI: 10.1016/j.omtn.2020.01.007
Cho, Lee, Shen et al
Mol Ther Nucleic Acids (2020) 19, 1186-1197 DOI: 10.1016/j.omtn.2020.01.007
Cited by 45
AI Summary: In this study, OriGene products including Human LEF1 cDNA (RC201375) and Anti-LEF1 antibody (TA324324) were used to overexpress and detect LEF1 in human mesenchymal stem cells, enabling the researchers to assess the therapeutic effects of LEF1-engineered cells in a rat model of myocardial infarction.
Epstein-Barr virus stably confers an invasive phenotype to epithelial cells through reprogramming of the WNT pathway
Birdwell, Prasai, Dykes et al
Oncotarget (2018) 9 (12), 10417-10435 DOI: 10.18632/oncotarget.23824
Birdwell, Prasai, Dykes et al
Oncotarget (2018) 9 (12), 10417-10435 DOI: 10.18632/oncotarget.23824
Snippet: 1 μg of plasmid DNA (LEF1: Origene, WNT5A: Addgene Supplementary Table 2), 5 μL/mL Dharmafect 1, in 600 μL of KSFM media was allowed to incubate on ice for 15 minutes before being added to cells for 5 hours. On the third day, 350 μg/mL of G418 was added. Stable cell lines generated consisted of pools of 2 more clones and were maintained on continuous selection
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
Corbett, Mann, Mann
PLoS ONE (2015) 10 (11), e0142794 DOI: 10.1371/journal.pone.0142794
Corbett, Mann, Mann
PLoS ONE (2015) 10 (11), e0142794 DOI: 10.1371/journal.pone.0142794
Cited by 42
AI Summary: OriGene products—specifically Wnt3a, Wnt5a, Wnt10b, LEF1, and sFRP4 constructs—were used to investigate the roles of canonical and non-canonical Wnt signaling in hepatic stellate cell activation and their effects on cell survival and paracrine signaling.
Snippet: Active β-catenin construct (S37A) was provided by D.L.Johnson, as used in Orford et al[42]. Wnt3a, Wnt5a, Wnt10b, LEF1 and sFRP4 constructs were sourced from Origene
Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation
Kasaai, Gaumond, Moffatt
J. Biol. Chem. (2013) 288 (19), 13278-94 DOI: 10.1074/jbc.M113.457010
Kasaai, Gaumond, Moffatt
J. Biol. Chem. (2013) 288 (19), 13278-94 DOI: 10.1074/jbc.M113.457010
Cited by 2
Showing 1-5 of 5 papers.
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