LEF1 (NM_016269) Human Untagged Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | LEF1 |
| Synonyms | LEF-1; TCF1ALPHA; TCF7L3; TCF10 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_016269 |
| Insert Size | 2660 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016269.2, NP_057353.1 |
| RefSeq Size | 3084 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 51176 |
| UniProt ID | Q9UJU2 |
| Cytogenetics | 4q25 |
| Domains | HMG |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway |
| Summary | This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2009 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016269" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208663 | LEF1 (Myc-DDK-tagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1 | 10 ug |
$686.00
|
|
| RC208663L1 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC208663L2 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC208663L3 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC208663L4 | Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG208663 | LEF1 (tGFP-tagged) - Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1 | 10 ug |
$489.00
$886.00
|
|
Citations
Chondroitin sulfates-mediated N-cadherin/β-catenin signaling is associated with basal-like breast cancer cell invasion
Nadanaka, Kinouchi, Kitagawa
J. Biol. Chem. (2017)
Nadanaka, Kinouchi, Kitagawa
J. Biol. Chem. (2017)
AI Summary: OriGene’s LEF expression vector (Cat. No. SC114370) was used in this study to investigate β-catenin signaling by co-transfection with a reporter vector in BT-549 breast cancer cells, enabling analysis of chondroitin sulfate-mediated effects on cell invasion.
Snippet: The pTCF7wt-luc reporter vector was co-transfected with the LEF expression vector (Cat. No. SC114370, ORIGENE) into BT-549 cells and the cells were treated with or without CS-E as indicated
LEF1 in androgen-independent prostate cancer: regulation of androgen receptor expression, prostate cancer growth, and invasion
Li, Wang, Zhang et al
Cancer Res (2009) 69 (8), 3332-8 DOI: 10.1158/0008-5472.CAN-08-3380
Li, Wang, Zhang et al
Cancer Res (2009) 69 (8), 3332-8 DOI: 10.1158/0008-5472.CAN-08-3380
AI Summary: OriGene products contributed to this study by providing LEF1 cDNA (cat# SC119785) for overexpression experiments and LEF1 shRNA (cat# TF311155) for gene knockdown, enabling the authors to investigate how LEF1 regulates androgen receptor expression and influences prostate cancer cell growth and invasion.
Showing 1-2 of 2 papers.
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