Cytokines

Circular map for MR227486

Ccl26 Mouse Gene Knockout Kit (CRISPR)

CCR5 Human Gene Knockout Kit (CRISPR)

Circular map for RC220974L4

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Coomassie blue staining of purified IFNA21 protein (Cat# TP310115). The protein was produced from HEK293T cells transfected with IFNA21 cDNA clone (Cat# RC210115) using MegaTran 2.0 (Cat# TT210002).

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human IL1B plasmid (RC202079, hIL1B), mouse IL1B plasmid (MR226719, mIL1B), rat IL1B plasmid (RR211023, rIL1B) using anti-IL1B antibody TA506443 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Circular map for RC202078

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40. Positive lysates LY400500 (100ug) and LC400500 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-OX40 (TNFRSF4) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800166) (1:300)

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506170).

Circular map for RC216785

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800095) (1:200)

Coomassie blue staining of purified IFNG protein (Cat# TP309993). The protein was produced from HEK293T cells transfected with IFNG cDNA clone (Cat# RC209993) using MegaTran 2.0 (Cat# TT210002).

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

Circular map for RC209993

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL6R (Cat# RC221874, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL6R antibody (Cat# TA506859). Positive lysates LY400192 (100ug) and LC400192 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

Circular map for RC202079

Coomassie blue staining of purified TNFRSF4 protein (Cat# TP311253). The protein was produced from HEK293T cells transfected with TNFRSF4 cDNA clone (Cat# RC211253) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC200410

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IL10RA (Cat# RC207020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL10RA(Cat# TA506929). Positive lysates LY400596 (100ug) and LC400596 (20ug) can be purchased separately from OriGene.

Circular map for RC202716

Circular map for RC208851

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-CD40 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Circular map for RC201167

LEP Antibody (N-term) (Cat. #AP52465PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescent staining of HeLa cells using anti-IL1F9 mouse monoclonal antibody (TA505994).

Circular map for RC211253

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CCL19 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Circular map for RC203799

Circular map for RC219855

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-OX40 (TNFRSF4) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800167) (1:50)

Circular map for RC202069

Circular map for RC209995

Circular map for RC215123

Circular map for RC205882

Circular map for RC219514

Circular map for RC207020

Circular map for RC202180

Circular map for RC221091