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HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DDX58 (Cat# RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58(Cat# TA506141). Positive lysates LY415370 (100ug) and LC415370 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

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Coomassie blue staining of purified ASL protein (Cat# TP301568). The protein was produced from HEK293T cells transfected with ASL cDNA clone (Cat# RC201568) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified MICA protein (Cat# TP329900). The protein was produced from HEK293T cells transfected with MICA cDNA clone (Cat# RC229900) using MegaTran 2.0 (Cat# TT210002).

SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-cJUN clone UMAB67 mouse monoclonal antibody. HIER ACCEL buffer (B22C-125) (pH8.7) at 110C for 10 min, UM800011 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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Coomassie blue staining of purified PGPEP1 protein (Cat# TP314837). The protein was produced from HEK293T cells transfected with PGPEP1 cDNA clone (Cat# RC214837) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified ADD2 protein (Cat# TP312102). The protein was produced from HEK293T cells transfected with ADD2 cDNA clone (Cat# RC212102) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified NDUFAF3 protein (Cat# TP317632). The protein was produced from HEK293T cells transfected with NDUFAF3 cDNA clone (Cat# RC217632) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified C1S protein (Cat# TP318963). The protein was produced from HEK293T cells transfected with C1S cDNA clone (Cat# RC218963) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified ARSE protein (Cat# TP324620). The protein was produced from HEK293T cells transfected with ARSE cDNA clone (Cat# RC224620) using MegaTran 2.0 (Cat# TT210002).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (RC223612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP. Positive lysates LY401576 (100ug) and LC401576 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. Positive lysates LY418732 (100ug) and LC418732 (20ug) can be purchased separately from OriGene.

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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYNN (RC207080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYNN. Positive lysates LY412964 (100ug) and LC412964 (20ug) can be purchased separately from OriGene.

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Coomassie blue staining of purified DNTTIP1 protein (Cat# TP306867). The protein was produced from HEK293T cells transfected with DNTTIP1 cDNA clone (Cat# RC206867) using MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified EVPLL protein (Cat# TP326700). The protein was produced from HEK293T cells transfected with EVPLL cDNA clone (Cat# RC226700) using MegaTran 2.0 (Cat# TT210002).

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DNA-binding activity of Androgen Receptor (AR) was measured in OriGene over-expression lysate LY423281 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing AR-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed AR is biologically active in the lysate. Overexpression cell lysates are prepared from HEK293T cells transfected with RC215709 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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