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CDK1 (NM_001786) Human Over-expression Lysate

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SKU
LY400676
Transient overexpression lysate of cyclin-dependent kinase 1 (CDK1), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200495
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001786, NP_001777
Synonyms CDC2; CDC28A; P34CDC2
Predicted MW 34.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001777
Locus ID 983
Cytogenetics 10q21.2
Protein Families WB
Protein Pathways Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation
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Other "CDK1" proteins (4)
SKU Description Size Price
PH300495 CDK1 MS Standard C13 and N15-labeled recombinant protein (NP_001777) 10 ug
$3,360.00
LC400676 Lyophilized CDK1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300495 Recombinant protein of human cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1, 20 µg 20 ug
$737.00
TP762400 Purified recombinant protein of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, full length, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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