CDK1 (NM_001786) Human Tagged ORF Clone
- TrueORF®
SKU
RC200495
CDK1 (Myc-DDK-tagged)-Human cyclin-dependent kinase 1 (CDK1), transcript variant 1
$300.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDK1 |
| Synonyms | CDC2; CDC28A; P34CDC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001786 |
| ORF Size | 891 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001786.5 |
| RefSeq Size | 1923 bp |
| RefSeq ORF | 894 bp |
| Locus ID | 983 |
| UniProt ID | P06493, I6L9I5 |
| Cytogenetics | 10q21.2 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation |
| MW | 34.1 kDa |
| Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001786" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200495L1 | Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200495L2 | Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC200495L3 | Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200495L4 | Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG200495 | CDK1 (tGFP-tagged) - Human cyclin-dependent kinase 1 (CDK1), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC111605 | CDK1 (untagged)-Human cyclin-dependent kinase 1 (CDK1), transcript variant 1 | 10 ug |
$300.00
|
|
| SC323387 | CDK1 (untagged)-Kinase deficient mutant (K33M) of Human cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
The Photorhabdus Virulence Cassettes RRSP-Like Effector Interacts With Cyclin-Dependent Kinase 1 and Causes Mitotic Defects in Mammalian Cells
Wang, Shen, Jiang et al
Front Microbiol (2020) 11, 366 DOI: 10.3389/fmicb.2020.00366
Wang, Shen, Jiang et al
Front Microbiol (2020) 11, 366 DOI: 10.3389/fmicb.2020.00366
Cited by 12
AI Summary: In this study, OriGene’s Flag-tagged CDK1 plasmid was used for co-transfection and pull-down assays to investigate the interaction between the RRSPPa effector and CDK1 in mammalian cells.
Snippet: For CDK1 immunoblotting, HeLa cells transfected with pEGFP and pEGFP-RRSPPa, pEGFP-RRSPPa H485A plasmids were lyzed and incubated with anti-GFP magnetic beads as mentioned above. Then, the eluents were subjected to Western blot probing with primary antibodies against CDK1 and EGFP. For the in vivo CDK1-RRSPPa pull-down assay, HeLa cells were cotransfected with EGFP-fused RRSPPa variants and Flag-tagged CDK1 (Origene). … For the detection of the CDK1 phosphorylation, the primary antibodies were used as follows: anti-CDK1, anti-CDK1 (phospho T161), and anti-GAPDH. …
Potent Activity of Composite Cyclin Dependent Kinase Inhibition against Hepatocellular Carcinoma
Shao, Li, Hsu et al
Cancers (Basel) (2019) 11 (10) DOI: 10.3390/cancers11101433
Shao, Li, Hsu et al
Cancers (Basel) (2019) 11 (10) DOI: 10.3390/cancers11101433
AI Summary: OriGene products contributed to this study by providing the CDK1 and CDK9 overexpression vectors used to assess the impact of these kinases on the efficacy of dinaciclib in hepatocellular carcinoma cells.
Snippet: In total, 1.75 × 10 5 HuH7 cells were seeded in 6-well plates and transfected with the indicated siRNA (100 nM) using the DharmaFECT 4 Transfection Reagent (Dharmacon) for 24 h. We purchased vectors overexpressing CDKs 1 and 9 from OriGene (Rockville, MD Cached;In total, 1.75 × 10 5 HuH7 cells were seeded in 6-well plates and transfected with the indicated siRNA (100 nM) using the DharmaFEC...
The role of p27(Kip1) in dasatinib-enhanced paclitaxel cytotoxicity in human ovarian cancer cells
Le, Mao, He et al
J Natl Cancer Inst (2011) 103 (18), 1403-22 DOI: 10.1093/jnci/djr280
Le, Mao, He et al
J Natl Cancer Inst (2011) 103 (18), 1403-22 DOI: 10.1093/jnci/djr280
Cited by 28
AI Summary: OriGene products, specifically human p27(Kip1) siRNA and Bcl-2 and Cdk1 expression vectors, were used in this study to manipulate gene expression and investigate the molecular mechanisms by which dasatinib enhances paclitaxel cytotoxicity in ovarian cancer cells.
Showing 1-3 of 3 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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