Metabolism

Western blot analysis of extracts (10ug) from Hela cell line by using anti-MGLL monoclonal antibody at 1:200 dilution.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2A6 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM4 (RC213480, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM4 (1:2000). Positive lysates LY422267 (100ug) and LC422267 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein NNMT from HELA lysates (0.5 mg/mL) using NNMT Mouse Monoclonal Antibody #TA502624. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ALOX15 plasmid (RC206621, lane 2), mouse ALOX15 plasmid (MR226258, lane 3) using anti-ALOX15 antibody TA504250 (1:500).

Simple Western™ analysis of endogenous protein PFKP from MCF-7 lysates (0.1 mg/mL) using PFKP Mouse Monoclonal Antibody #TA503982. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein BCAT1 from HepG2 lysates (0.5 mg/mL) using BCAT1 Mouse Monoclonal Antibody #TA504360. The virtual lane view (left) shows the target (as indicated) at 1:20 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:20 dilutios of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates LY400290 (100ug) and LC400290 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PANK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GBA mouse monoclonal antibody. (UM800064; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP2B6 (Cat# RC217236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2B6(Cat# TA504328). Positive lysates LY400259 (100ug) and LC400259 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein GLB1 from HELA lysates (0.2 mg/mL) using GLB1 Mouse Monoclonal Antibody #TA505544. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein AFP from HepG2 lysates (0.2 mg/mL) using AFP Mouse Monoclonal Antibody #TA501782. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CAT (Cat# RC210763, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAT. (1:5 (Cat# TA502496). Positive lysates LY419766 (100ug) and LC419766 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY QPRT (Cat# RC202960, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-QPRT (Cat# TA501524). Positive lysates LY402307 (100ug) and LC402307 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (RC219187, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS. Positive lysates LY424863 (100ug) and LC424863 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein GPI from HELA lysates (0.2 mg/mL) using GPI Mouse Monoclonal Antibody #TA501171. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IDS (Cat# RC219187, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS(Cat# TA504277). Positive lysates LY424863 (100ug) and LC424863 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein Adenylosuccinate Lyase (ADSL) from HELA lysates (0.1 mg/mL) using ADSL Mouse Monoclonal Antibody #TA501810. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein SMPD1 from HepG2 lysates (0.2 mg/mL) using SMPD1 Mouse Monoclonal Antibody #TA803659. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human EPHX2 plasmid (RC202489, hEPHX2), mouse EPHX2 plasmid (MR208783, mEPHX2) using anti-EPHX2 antibody TA501626(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAT (RC210763, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAT. (1:5. Positive lysates LY419766 (100ug) and LC419766 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded carcinoma of human kidney tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800070 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GALE mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ADH1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Simple Western™ analysis of endogenous protein LCMT1 from HELA lysates (0.2 mg/mL) using LCMT1 Mouse Monoclonal Antibody #TA503125. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein IDH1 from HepG2 lysates (0.2 mg/mL) using IDH1 Mouse Monoclonal Antibody #TA500610. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein CTH from HELA lysates (1 mg/mL) using CTH Mouse Monoclonal Antibody #TA502442. The virtual lane view (left) shows the target (as indicated) at a 1:30 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:30 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPAT (Cat# RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT(Cat# TA504769). Positive lysates LY400951 (100ug) and LC400951 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RDH11 plasmid (RC205003, hRDH11), mouse RDH11 plasmid (MR204536, mRDH11) using anti-RDH11 antibody TA501053 (1:20000@1mg/ml). Figure B, Western blot analysis of the same human RDH11 and mouse RDH11 samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GLUL plasmid (RC204161, hGLUL), mouse GLUL plasmid (MR205788, mGLUL) using anti-GLUL antibody TA500700 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SMS (Cat# RC200619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS(Cat# TA503099). Positive lysates LY417877 (100ug) and LC417877 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein DHFR from HELA lysates (0.2 mg/mL) using DHFR Mouse Monoclonal Antibody #TA500517. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TBXAS1 (Cat# RC208028, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBXAS1(Cat# TA501358). Positive lysates LY420741 (100ug) and LC420741 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GATM (Cat# RC200474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATM(Cat# TA503207). Positive lysates LY419923 (100ug) and LC419923 (20ug) can be purchased separately from OriGene.

Anti-ACLY mouse monoclonal antibody (TA501031) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY (RC200508).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYP17A1 (Cat# RC209042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1(Cat# TA503442). Positive lysates LY400030 (100ug) and LC400030 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GCK (Cat# RC200472, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK(Cat# TA500459). Positive lysates LY400059 (100ug) and LC400059 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NEU1 (Cat# RC200386, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU1(Cat# TA801727). Positive lysates LY424720 (100ug) and LC424720 (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1 (RC202440) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ALDH3A1 mouse monoclonal (TA501105), and then analyzed by flow cytometry.

Recombinant Hu LDHA protein (TP309378) 100 ng/mL was coated on plate and incubated at 4°C overnight. After blocking with 1% BSA, primary antibody TA500568 was added as a standard curve with a 2x serial dilution from 50 ng/mL, and 10,000x diluted HRP conjugated goat anti-mouse IgG was used as a detection antibody. S/B = 44.24, EC50 = 34.22 ng/mL

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PGM3 (Cat# RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3(Cat# TA504859). Positive lysates LY414446 (100ug) and LC414446 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DGKA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.