Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: OTI2H9]
SKU
TA500610
Anti-IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)
$447.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI2H9 |
|---|---|
| Application | IF, Simple Western, WB |
| Recommended Dilution | WB 1:2000, IHC 1:50, IF 1:100, Simple Western 1:20 - 1:50 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full-length protein expressed in 293T cell transfected with human IDH1 expression vector |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 46.7 kDa |
| Gene Name | isocitrate dehydrogenase (NADP(+)) 1 |
| Database Link | |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Reference Data | |
| Protein Categories | Cancer: Brain and CNS, Cancer: Haematopoietic and Lymphoid, Cardiovascular diseases, Intracellular Proteins |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2H9" antibodies (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF500610 | Carrier-free (BSA/glycerol-free) IDH1 mouse monoclonal antibody, clone OTI2H9 (formerly 2H9) | 100 ug |
$620.00
|
|
| TA400008 | Anti-IDH1 mouse mAb, clone OTI2H9, Biotinylated | 100 ul |
$495.00
|
|
| TA400009 | Anti-IDH1 mouse mAb, clone OTI2H9, HRP conjugated | 100 ul |
$495.00
|
|
| TA400028 | Anti-IDH1 mouse mAb, clone OTI2H9, DyLight488 conjugated | 100 ul |
$495.00
|
|
| TA500610S | Anti-IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, clone OTI2H9 (formerly 2H9) | 30 ul |
$200.00
|
|
| TA600198 | IDH1 mouse monoclonal capture antibody, validated for ELISA and Luminex assays | 100 ug |
$458.00
|
|
| TA700198 | IDH1 biotinylated mouse monoclonal detection antibody, validated for ELISA and Luminex assays | 50 ug |
$494.00
|
|
Citations
A High-Throughput Fluorimetric Assay for 2-hydroxyglutarate Identifies Zaprinast as a Glutaminase Inhibitor
Elhammali, Ippolito, Collins et al
Cancer Discov (2014)
Elhammali, Ippolito, Collins et al
Cancer Discov (2014)
HuR is a post-transcriptional regulator of core metabolic enzymes in pancreatic cancer
Burkhart, Pineda, Chand et al
RNA Biol (2013) 10 (8), 1312-23 DOI: 10.4161/rna.25274
Burkhart, Pineda, Chand et al
RNA Biol (2013) 10 (8), 1312-23 DOI: 10.4161/rna.25274
Cited by 66
AI Summary: In this study, OriGene’s HuR shRNA (Cat# TL311089) and HuR overexpression plasmid (Cat# SC119295) were used to manipulate HuR expression levels in pancreatic cancer cell lines, enabling the authors to demonstrate HuR’s critical role in regulating core metabolic enzymes and cell survival under glucose deprivation.
A histopathological diagnostic marker for human spinal astrocytoma: expression of glial fibrillary acidic protein-δ
Heo, Kim, Yang et al
J. Neurooncol. (2012) 108 (1), 45-52 DOI: 10.1007/s11060-012-0801-z
Heo, Kim, Yang et al
J. Neurooncol. (2012) 108 (1), 45-52 DOI: 10.1007/s11060-012-0801-z
Cited by 29
AI Summary: OriGene contributed to this study by providing the IDH1 antiserum (catalog no. not specified), which was used for immunohistochemical analysis of spinal cord astrocytoma samples.
Snippet: IDH1 antiserum was purchased from OriGene (catalog no. ... J 18:1397–1422 4. Garcs-Ambrossi GL, McGirt MJ, Mehta VA, Sciubba DM, Wi- tham TF, Bydon A, Wolinksy J, Jallo GI, Gokaslan ZL (2009) Factors associated with progression-free survival and long-term neurological
Showing 1-3 of 3 papers.
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